Appraisal of disease-modifying potential of amlodipine as an anti-arthritic agent: new indication for an old drug

Qasim, Sumera; Alamgeer, -; Kalsoom, Saima; Shahzad, Muhammad; Irfan, Hafiz Muhammad; Zafar, Muhammad Shoaib; Bukhari, Ishfaq A.; Vohra, Fahim; Afzal, Sibtain

doi:10.1007/s10787-020-00692-9

Cited by 18 publications

(15 citation statements)

References 42 publications

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“…After 28 days, ankle joints of all rats included in different treatment groups were dissected for radiographic analysis, and afterward, histological analysis was performed for these ankle joints. 39 Inflammatory cell infiltration, bone erosion, and pannus formation were analyzed by a blinded histopathologist. Scores from 0 to 4 were given according to the following criterion: 0 for normal, 1 for minimal, 2 for mild, 3 for moderate, and 4 for severe changes.…”

Section: Methodsmentioning

confidence: 99%

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Rosuvastatin Attenuates Rheumatoid Arthritis-Associated Manifestations via Modulation of the Pro- and Anti-inflammatory Cytokine Network: A Combination of In Vitro and In Vivo Studies

et al. 2021

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The current investigation employed rosuvastatin for evaluation as an antiarthritic agent by in vitro and in vivo studies. In vitro studies comprised egg albumin and bovine serum albumin protein denaturation assays along with membrane stabilization assays, while in vivo studies comprised formaldehyde and complete Freund’s adjuvant (CFA)-provoked arthritis. The antioxidant potential was estimated via DPPH free radical scavenging and ferric reducing assays. Rosuvastatin significantly inhibited heat-provoked protein denaturation of egg albumin and bovine serum in a concentration-dependent way with the highest inhibition of 1225 ± 9.83 and 82.80 ± 4.03 at 6400 μg/mL. The percentage protection of the RBC membrane from hypotonicity-prompted lysis was found to be 80.67 ± 2.7. Rosuvastatin promisingly subdued formaldehyde-provoked arthritis, with maximum reduction (65.47%) of the paw volume being observed at a dose of 40 mg/kg. Rosuvastatin also significantly (p < 0.001) attenuated arthritis induced by CFA injection by reducing the paw volume and arthritic index. The reduction in the body weight due to CFA injection was also preserved by rosuvastatin treatment. Hematological and biochemical changes due to arthritis induction by CFA injection were also maintained near normal values by rosuvastatin. The histopathological and radiographic investigation also revealed the protective effect of rosuvastatin on preventing structural changes. Gene expression of IL-1β, TNF-α, and IL-6 was reduced, while IL-4 and IL-10 levels were elevated by rosuvastatin in comparison to those for the disease control group. Concentration-dependent antioxidant potential was shown by rosuvastatin. Thus, rosuvastatin possesses a notable antiarthritic potential as evidenced via in vitro and in vivo studies.

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Section: Methodsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Rosuvastatin Attenuates Rheumatoid Arthritis-Associated Manifestations via Modulation of the Pro- and Anti-inflammatory Cytokine Network: A Combination of In Vitro and In Vivo Studies

et al. 2021

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“…Each paw was observed visually, and a score was given according to the criteria mentioned in Table . The maximum score for the arthritic index was 16/animal …”

Section: Methodsmentioning

confidence: 99%

“…Paw volume was appraised on a weekly basis with a digital plethysmometer to elucidate the extent of paw edema provoked by CFA injection and suppressed by different treatments. Percentage inhibition of CFA-provoked arthritis was estimated according to the following formula: where V c is the paw volume of the control group and V t is the paw volume of the treatment-provided groups.…”

Section: Methodsmentioning

confidence: 99%

“…Rats ankle joints excised after the completion of the study were taken for radiographic investigation and later for histological assessment. , Radiographic examination was done with a computerized radiographic system (Toshiba 630 MA, DS-TA-5A). For histopathological assessment, ankle joints were placed in 10% formalin for fixation; later on, after five days, they were decalcified using a decalcification solution.…”

Section: Methodsmentioning

confidence: 99%

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Appraisal of the Antiarthritic Potential of Prazosin via Inhibition of Proinflammatory Cytokine TNF-α: A Key Player in Rheumatoid Arthritis

et al. 2021

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Prazosin, a selective α1 adrenergic receptor antagonist, with documented anti-inflammatory potential, was evaluated for its antiarthritic efficacy by targeting specifically TNF-α. The antiarthritic attribute of prazosin validated through in vitro screening comprised thermally provoked denaturation of bovine serum albumin (BSA) and egg albumin along with membrane stabilization evaluation at a concentration of 100–6400 μg/mL, while in vivo screening comprised formaldehyde-instigated arthritis at the doses of 5, 10, and 20 mg/kg and complete Freund’s adjuvant (CFA)-induced arthritis at 20 mg/kg dose. Paw swelling, body weight, arthritic score, hematological parameters, and histological and radiographic examination of ankle joints were assessed for a period of 28 days after CFA immunization. Moreover, the proinflammatory cytokine TNF-α level was also assessed through quantitative real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Prazosin revealed significant antiarthritic effect evident through protein denaturation inhibition in the egg albumin and the BSA model, stabilization of red blood cell membrane in the membrane stabilizing assay, and reduction in paw volume in formaldehyde-induced arthritis. Likewise, prazosin exhibited propitious antiarthritic effects in the CFA-provoked arthritis model manifested by paw volume and arthritic score alleviation, substantial weight loss prevention, and preservation of the normal hematological and biochemical profile. Histological and X-ray investigation unveiled no substantive structural alterations in treated rat’s ankle joints. The TNF-α expression level was also reduced. Thus, the current study is suggestive that prazosin exhibits a strong antiarthritic potential possibly through inhibition of TNF-α.

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Pharmacological evaluation of anti-arthritic potential of terpinen-4-ol using in vitro and in vivo assays

et al. 2022

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Appraisal of disease-modifying potential of amlodipine as an anti-arthritic agent: new indication for an old drug

Cited by 18 publications

References 42 publications

Rosuvastatin Attenuates Rheumatoid Arthritis-Associated Manifestations via Modulation of the Pro- and Anti-inflammatory Cytokine Network: A Combination of In Vitro and In Vivo Studies

Rosuvastatin Attenuates Rheumatoid Arthritis-Associated Manifestations via Modulation of the Pro- and Anti-inflammatory Cytokine Network: A Combination of In Vitro and In Vivo Studies

Appraisal of the Antiarthritic Potential of Prazosin via Inhibition of Proinflammatory Cytokine TNF-α: A Key Player in Rheumatoid Arthritis

Pharmacological evaluation of anti-arthritic potential of terpinen-4-ol using in vitro and in vivo assays

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