Appraisal of fluorimetric assay of aryl hydrocarbon (benzo(α)pyrene) hydroxylase in cultured human lymphocytes

Trieff, Norman M.; Forti, G. Cantelli; Smart, Vera B.; Kempen, Rene R.; Kilian, D.J.

doi:10.1038/bjc.1978.207

Cited by 4 publications

(1 citation statement)

References 16 publications

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“…The dose of BP was calculated according to the recommended in vivo dosage of 2.5 mg/kg [38] . The purification of (G-3H)-BP and of unlabeled BP was carried out according to our previous description [39] and the acetone solution of BP + (G-3 H)-BP was prepared and stored at -I 5°C until use. vals (between 1and 8-minute intervals as the perfusion progressed, with more frequent collection initially) and bile collected every 15 minutes.…”

Section: Animalsmentioning

confidence: 99%

Kinetics of uptake and biliary excretion of benzo(α)pyrene and mutagenic metabolites in isolated perfused rat liver

Forti

Trieff

1981

Teratog Carcinog Mutagen

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An isolated liver perfusion system was used as a simplifying tool to study the metabolism and excretion of benzo(alpha)pyrene (BP) as a prototype carcinogen/mutagen. Phenobarbital (PB) was used to induce liver microsomal enzymes in Sprague-Dawley male rats prior to isolated liver perfusion. Control livers were run simultaneously using generally tritiated (G-3H)BP/BP as substrate in the perfusion medium. Both biliary excretion and liver weight were increased in the induced compared to control liver, but biliary flow when corrected for liver weight is statistically the same for both control and PB-induced livers. The excretion rat of radioactivity in the bile is always higher for PB-induced than for control liver (maximum radioactive excretion at 1 hr). There is a more rapid radioactivity removal in the liver perfusion medium for PB-induced than for control livers. Data are explained by increased metabolism of BP in induced liver leading to the presence of more polar metabolites undergoing preferential biliary excretion than in the control liver. Results support in vivo experimental data. Extracts from liver and bile were tested for microbial mutagenicity by the Ames test (TA 100) after TLC separation. The control liver shows virtually no mutagenicity in bile, only in TLC fractions from the liver. The PB-induced liver shows significant mutagenicity in several TLC fractions in both bile and liver. The net effect of induction is to produce more mutagenic metabolites of BP, excreted in the bile, and presenting a significant exposure of carcinogens/mutagens, and consequent hazard to man.

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Section: Animalsmentioning

confidence: 99%

Kinetics of uptake and biliary excretion of benzo(α)pyrene and mutagenic metabolites in isolated perfused rat liver

Forti

Trieff

1981

Teratog Carcinog Mutagen

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Sister Chromatid Exchanges

Latt

Schreck

Loveday

et al. 1980

Advances in Human Genetics 10

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The metabolism of 7-ethoxycoumarin in primary maintenance cultures of adult rat hepatocytes

Fry

Bridges

1980

Naunyn-Schmiedeberg's Arch. Pharmacol.

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7-Ethoxycoumarin is metabolized to 7-hydroxycoumarin in short-term (1-4 days) maintenance cultures of adult rat hepatocytes. The 7-hydroxycoumarin is predominantly found as the sulphate and glucuronic acid conjugates. This pattern of metabolism is very similar to that observed with freshly-isolated rat hepatocytes and suggests that the culture system may be of value in studying the metabolism of novel chemicals designed for human therapeutic use. The specific activity of microsomal monooxygenase activity falls by 50-60% during 4 days in culture. This is not reflected by the sulphate and glucuronic acid conjugation pathways which are retained at normal levels throughout the entire 4-day culture period.

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Appraisal of fluorimetric assay of aryl hydrocarbon (benzo(α)pyrene) hydroxylase in cultured human lymphocytes

Cited by 4 publications

References 16 publications

Kinetics of uptake and biliary excretion of benzo(α)pyrene and mutagenic metabolites in isolated perfused rat liver

Kinetics of uptake and biliary excretion of benzo(α)pyrene and mutagenic metabolites in isolated perfused rat liver

Sister Chromatid Exchanges

The metabolism of 7-ethoxycoumarin in primary maintenance cultures of adult rat hepatocytes

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