Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of FolkloricLannea coromandelicaUsingIn VitroandIn VivoAssays

Kumar, T. Kranthi; Jain, Vishal

doi:10.1155/2015/203679

Cited by 36 publications

(27 citation statements)

References 62 publications

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“…Chromatographic techniques include gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), high-pressure liquid chromatography (HPLC), and liquid chromatography-mass spectrometry (LC-MS) [14]. Various spectroscopic techniques used to determine TPC are Fourier Transform Infrared (FT-IR) spectroscopy, Raman spectroscopy, and Folin-Ciocalteu (F-C) assay using UV-VIS spectroscopy [15] [16]. TPC is also indirectly measured in terms of antioxidant activity (AOA) of an extract as results have shown that there is a direct correlation of AOA and TPC content [17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Standards and Interfering Compounds in the Determination of Phenolics by Folin-Ciocalteu Assay Method for Effective Bioprocessing of Biomass

Bastola¹,

Guragain²,

Bhadriraju³

et al. 2017

AJAC

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Folin Ciocalteu (F-C) assay is the most widely used and convenient method to determine the total phenolics content in foods, herbs, and other plant extracts. Different phenolics standards such as gallic acid, ferulic acid, chlorogenic acid, catechol, and vanillic acid have been used for calibration curves in this assay method. Comparison of these standards, in single or combination of two or more, for more accurate determination of phenolics has not been reported so far. This study tested five single phenolics and seven combinations of mixed phenolic standards to evaluate the optimal standards for F-C method. The different standards were tested to calculate the phenolic content in three known test solutions. We also evaluated interference effect of various compounds in phenolics estimation by F-C method that is usually present in the lignocellulosic biomass-derived sugar solution, and in food products along with phenolics. Finally, the optimal standards with five phenolics were used for the determination of phenolics in alkali pretreated biomass extract. The results indicated that gallic acid was the best standard among the single phenolic compounds and five phenolic compounds solution was the best standard among the mixed phenolic solutions. The presence of glucose, HMF, furfural, and vitamin-B12 did not interfere in phenolic determination; whereas ascorbic acid, tyrosine, formic acid, and acetic acid strongly interfered the results. The results also showed that biomass pelleting process did not affect the generation of phenolics in alkali pretreatment extract.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Standards and Interfering Compounds in the Determination of Phenolics by Folin-Ciocalteu Assay Method for Effective Bioprocessing of Biomass

Bastola¹,

Guragain²,

Bhadriraju³

et al. 2017

AJAC

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“…The absorbance for extract was 1.003 and 1.593 for the standard. From the results obtained in this study, it is suggested that the ethanolic extract potentially donates the electron to reactive free radicals to convert them into more stable nonreactive form and terminates the free radical chain reaction [34].…”

Section: Biswas and Senmentioning

confidence: 78%

Pharmacognostical Evaluation, in Vitro Antioxidant Effects of Syzygium Cumini Linn. Seed Extract, and the Potential Role of This Extract as Hypoglycemic Agent in Alloxan–induced Diabetic Rats

Biswas¹,

Sen²

2018

Asian J Pharm Clin Res

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Objective: Diabetes, the “third killer” of mankind, is now a global burden affecting nearly 10% of the population. The present study aimed at pharmacognostical evaluation of Syzygium cumini seed powder, phytochemical profiling, total phenolic, and flavonoid content determination and to ascertain its antioxidant activity and hypoglycemic potential in alloxan-induced diabetic rats.Methods: Pharmacognostical and physicochemical parameters of the seed powder were determined by using standard methods as per Ayurvedic Pharmacopoeia of India (API). In vitro antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Ethanolic extract of Syzygium cumini seed at a dose of 200mg/kg and 400mg/kg body weight (bw) were given orally in alloxan-induced (150mg/kg, i.p) diabetic rats daily for three weeks.Results: physicochemical parameters complied with the API standards. Phytochemical profiling revealed that the extract contains phenolic compounds, flavonoids, glycosides, alkaloids, tannins, and saponins. The extract is rich in phenolic compounds which were 177.33 mg gallic acid equivalent/g (GAE/g dry weight) and showed significant percentage inhibition compared to standard ascorbic acid. The extract reduced the fasting blood sugar (FBS) level by 46.67–52.67% which is very close to the standard drug glibenclamide (reduced FBS by 65.58%). It also improved the bw by 18.20–20.41% after extract treatment where the standard drug increased the bw by 22.95% of the diabetic rats.Conclusion: In conclusion, Syzygium cumini seed extract is rich in phenolic compounds. Results suggested that the extract possesses significant radical (DPPH) scavenging activity and FBS lowering potential in alloxan-induced diabetic rats.

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“…It was found that the TPs significantly decreased hydroxyl radical concentration in a dose-dependent manner at the test concentrations. Superoxide radicals cause oxidative damage to many biomolecules and are the most common free radicals produced in vivo by several oxidative enzymes [23] . The ability of the TPs to scavenge superoxide radicals was determined by the reduction of NBT.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Total Polyphenols Extraction from Vigna angularis and Their Antioxidant Activities

Zhang¹,

Wang²

2016

IJPS

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Zhang and Wang: Total Polyphenols from Vigna angularis and their EffectsVigna angularis, also called adzuki bean, a member of the family Fabaceae, has long been cultivated in Asian countries. This legume has received considerable attention owing to the presence of polyphenols. The optimal extraction conditions for total polyphenols from V. angularis and their antioxidant activities were investigated in this study. Ultrasound-assisted extraction was used to optimize the extraction of total polyphenols from V. angularis. Factors that influenced the extraction efficiency, such as ethanol concentration, liquid-solid ratio, and ultrasonic time were investigated by Box-Behnken design. Folin-Ciocalteu colorimetry was adopted to determine the total polyphenol content by using gallic acid as a reference. Antioxidant activities of the total polyphenols were evaluated by 1,1-diphenyl-2-picrylhydrazyl, hydroxyl and superoxide radical-scavenging activity assays. The optimized extraction conditions were as follows: ethanol concentration of 44.3%; liquidsolid ratio of 21.6:1 and ultrasonic extraction time of 30 min. Under these optimal conditions, the model's predicted extraction efficiency was 4.19 mg/g. The mathematical model had a high correlation (P<0.05) and could be employed to extract total polyphenols from V. angularis. In vitro antioxidant activity results showed that total polyphenols had strong antioxidant capacity and could be used as potential antioxidant agents. This study provided a reference for the exploration of new natural antioxidants. Key words: Vigna angularis (Adzuki bean), total polyphenols, extraction, Box-Behnken design, response surface methodology, antioxidant activityThe extraction of phytochemicals from food plants using solvents is an important step in separation and bioactivity research. Thus, efficient extraction techniques are required to harvest the natural products. Ultrasound-assisted extraction (UAE) is an efficient extraction technique that can help solutes more rapidly diffuse from the solid material into the solvent [1] . UAE has been widely used owing to its high efficiency, shorter processing time and reduced solvent consumption.Vigna angularis, also called adzuki beans, a member of the family Fabaceae, is an important food crop and used as folk medicines in Asian countries [2] . V. angularis have relatively small beans (about 5 mm). Most of the adzuki beans cultivated in Eastern Asian countries are red in color. This legume is rich in dietary fibers, proteins, minerals, vitamin A, vitamin B9 and folate [3] . Due to its biochemical components, V. angularis has been evaluated as a new treatment option. In addition, it has received considerable attention owing to the presence of polyphenols, such as proanthocyanidins and quercetin [4] . Numerous studies have demonstrated that polyphenols possess antioxidant [5,6] , anticarcinogenic [7,8] , antiglycation [9] and neuroprotective [10] properties. However, there are no data relating to optimized extraction conditions of total polyphenols (T...

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Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of FolkloricLannea coromandelicaUsingIn VitroandIn VivoAssays

Cited by 36 publications

References 62 publications

Evaluation of Standards and Interfering Compounds in the Determination of Phenolics by Folin-Ciocalteu Assay Method for Effective Bioprocessing of Biomass

Evaluation of Standards and Interfering Compounds in the Determination of Phenolics by Folin-Ciocalteu Assay Method for Effective Bioprocessing of Biomass

Pharmacognostical Evaluation, in Vitro Antioxidant Effects of Syzygium Cumini Linn. Seed Extract, and the Potential Role of This Extract as Hypoglycemic Agent in Alloxan–induced Diabetic Rats

Optimization of Total Polyphenols Extraction from Vigna angularis and Their Antioxidant Activities

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