Approaches for the Development of Rapid Serological Assays for Surveillance and Diagnosis of Infections Caused by Zoonotic Flaviviruses of the Japanese Encephalitis Virus Serocomplex

Hobson‐Peters, Jody

doi:10.1155/2012/379738

Cited by 32 publications

(24 citation statements)

References 170 publications

(192 reference statements)

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“…This test is a highly valuable tool because IgM antibodies are hard to detect using VNTs or HITs [133,135] and exhibit less cross-reactivity than IgG antibodies [136,137]. …”

Section: Serological Cross-reactions Between West Nile and Relatedmentioning

confidence: 99%

Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and Control of West Nile Disease

Beck

Jiménez‐Clavero²,

Leblond

et al. 2013

IJERPH

153

150

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In Europe, many flaviviruses are endemic (West Nile, Usutu, tick-borne encephalitis viruses) or occasionally imported (dengue, yellow fever viruses). Due to the temporal and geographical co-circulation of flaviviruses in Europe, flavivirus differentiation by diagnostic tests is crucial in the adaptation of surveillance and control efforts. Serological diagnosis of flavivirus infections is complicated by the antigenic similarities among the Flavivirus genus. Indeed, most flavivirus antibodies are directed against the highly immunogenic envelope protein, which contains both flavivirus cross-reactive and virus-specific epitopes. Serological assay results should thus be interpreted with care and confirmed by comparative neutralization tests using a panel of viruses known to circulate in Europe. However, antibody cross-reactivity could be advantageous in efforts to control emerging flaviviruses because it ensures partial cross-protection. In contrast, it might also facilitate subsequent diseases, through a phenomenon called antibody-dependent enhancement mainly described for dengue virus infections. Here, we review the serological methods commonly used in WNV diagnosis and surveillance in Europe. By examining past and current epidemiological situations in different European countries, we present the challenges involved in interpreting flavivirus serological tests and setting up appropriate surveillance programs; we also address the consequences of flavivirus circulation and vaccination for host immunity.

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“…This test is a highly valuable tool because IgM antibodies are hard to detect using VNTs or HITs [133,135] and exhibit less cross-reactivity than IgG antibodies [136,137]. …”

Section: Serological Cross-reactions Between West Nile and Relatedmentioning

confidence: 99%

Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and Control of West Nile Disease

Beck

Jiménez‐Clavero²,

Leblond

et al. 2013

IJERPH

153

150

View full text Add to dashboard Cite

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“…The symptoms of infection in humans ranged from a simple febrile headache to meningoencephalitis, and the severity of the symptoms increases with the patient age (1)(2)(3)(4)(5). Cross-reactivity in diagnostic assays between SLEV and other flaviviruses complicates differential diagnosis (6). This is particularly challenging in Argentina where there are dengue virus (DENV) outbreaks frequently, history of jungle yellow fever, and Zika virus (ZIKV) activity was demonstrated and YFV vaccination is common in some regions (7)(8)(9)(10) Regarding the specific antiviral response pattern against SLEV, IgM antibodies persistence has been detected in some patients showing that the presence of IgM might not be indicative of acute infection (11,12).…”

Section: Manuscriptmentioning

confidence: 99%

Specific diagnostic method for St. Louis Encephalitis Virus using a non-structural protein as antigen

Simari

Goñi

Luppo

et al. 2019

Preprint

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St. Louis encephalitis virus (SLEV) is a mosquito-borne reemerging flavivirus in Argentina. It is currently necessary to develop specific serological tests that can efficiently discriminate the flaviviruses that circulate in our country. The immunoassays to diagnose SLEV lack specificity because they are based on the detection of structural viral proteins and the human immunoglobulins produced during infection against these proteins cross-react with other flaviviruses. Here, we describe an enzyme-immunoassay designed to detect human IgG antibodies specific to the viral nonstructural protein NS5. The results indicate that NS5 is a promising antigen useful to discriminate SLEV from other circulating flaviviruses.

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“…This alteration contributes for the formation and activation of E protein homodimers. Researchers suggested that the extremely conserved N glycosylation motif N 15 -X 16 -T 17 in JEV PrM and its N-glycan substituents are essential for several stages of JEV biology: PrM biogenesis, virus release and pathogenesis. The E protein is the largest structural protein, comprising of approximately 500 aminoacids, with up to two potential glycosylation sites.…”

Section: Viral Replication and Morphogenesismentioning

confidence: 99%

Japanese Encephalitis: A Neglected Viral Disease and Its Impact on Global Health

Saxena¹,

Agrawal²,

Nair³

2014

Trends in Infectious Diseases

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Approaches for the Development of Rapid Serological Assays for Surveillance and Diagnosis of Infections Caused by Zoonotic Flaviviruses of the Japanese Encephalitis Virus Serocomplex

Cited by 32 publications

References 170 publications

Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and Control of West Nile Disease

Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and Control of West Nile Disease

Specific diagnostic method for St. Louis Encephalitis Virus using a non-structural protein as antigen

Japanese Encephalitis: A Neglected Viral Disease and Its Impact on Global Health

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