Approaches for the inhibition and elimination of microbial biofilms using macromolecular agents

Blackman, Lewis D.; Qu, Yue; Cass, Peter; Locock, Katherine E. S.

doi:10.1039/d0cs00986e

Cited by 108 publications

(91 citation statements)

References 208 publications

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“…Biofilm formation is a complex cooperative group process, which occurs in step-by-step processes and involves chemical communication within and between cells. Biofilm formation proceeds in four stages: adhesion, microcolony formation, maturation, and dispersion [4].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms

Bisso

Kuaté

Dzoyem

2021

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. Thymol and piperine are two naturally occurring bioactive compounds with several pharmacological activities. In this study, their antibiofilm potential either alone or in combination with three aminoglycoside antibiotics was evaluated against a biofilm of Klebsiella pneumoniae. Methods. Determination of antimicrobial susceptibility was performed using the broth microdilution method. Biofilm formation was evaluated by the microtiter plate method. Antibiofilm activity was determined using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium-bromide (MTT) assay. The combination studies were performed by the checkerboard microdilution method. Results. The minimum biofilm inhibitory concentration (MBIC) of streptomycin was reduced by 16- to 64-fold when used in combination with thymol, while the MBIC of kanamycin was reduced by 4-fold when combined with piperine. The minimum biofilm eradication concentration (MBEC) values of streptomycin, amikacin, and kanamycin were, respectively, 16- to 128-fold, 4- to 128-fold, and 8- to 256-fold higher than the planktonic minimum inhibitory concentration (MIC). Thymol combined with streptomycin or kanamycin showed synergic effects against the preformed biofilm with 16- to 64-fold reduction in the minimum biofilm eradication concentration values of each antibiotic in combination. Piperine acted also synergically with kanamycin with an 8- to 16-fold reduction in the minimum biofilm eradication concentration values of kanamycin in combination. Conclusion. The association of thymol with antibiotics showed a strong synergistic effect both in the inhibition of biofilm formation and the destruction of the preformed biofilm of K. pneumoniae. This study suggests that a combination of thymol with streptomycin, amikacin, or kanamycin could be a promising alternative therapy to overcome the problem of K. pneumoniae biofilm-associated infections.

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Section: Introductionmentioning

confidence: 99%

Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms

Bisso

Kuaté

Dzoyem

2021

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Synthetic NPs have the advantages of increased drug loading, special release properties, and diverse engineering functions; therefore, they are widely studied and used in biomedicine ( Swain et al, 2016 ). However, there remain limitations such as immunogenicity in applying NPs ( Blackman et al, 2021 ). NPs are initially coated with a variety of polymer molecules, including natural substances such as polysaccharides or semi-synthetic substances such as polymers, which are used to make the NPs as biofriendly and immune system evasive as possible.…”

Section: Introductionmentioning

confidence: 99%

Research Progress on Cell Membrane-Coated Biomimetic Delivery Systems

Guo

Xia

et al. 2021

Front. Bioeng. Biotechnol.

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Cell membrane-coated biomimetic nanoplatforms have many inherent properties, such as bio-interfacing abilities, self-identification, and signal transduction, which enable the biomimetic delivery system to escape immune clearance and opsonization. This can also maximize the drug delivery efficiency of synthetic nanoparticles (NPs) and functional cell membranes. As a new type of delivery system, cell membrane-coated biomimetic delivery systems have broadened the prospects for biomedical applications. In this review, we summarize research progress on cell membrane biomimetic technology from three aspects, including sources of membrane, modifications, and applications, then analyze their limitations and propose future research directions.

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“…[ 20,27 ] The presence of cationic charge helps AMPs to interact with the EPS matrix of the biofilm to eradicate it. [ 28,29 ] In the recent past, AMP mimicking antimicrobial polymer development has appeared as one of the prominent applicants to control the growing antimicrobial resistance owing to its simple and scalable preparation. [ 30–33 ] Due to the distribution of hydrophilic cationic charge and lipophilic long chain over the polymer backbone, many of these polymers can self‐assemble in appropriate solvent to produce the antimicrobial polymeric nanoaggregates.…”

Section: Introductionmentioning

confidence: 99%

Macromolecular Nanotherapeutics and Antibiotic Adjuvants to Tackle Bacterial and Fungal Infections

Dey

Mukherjee

Barman

et al. 2021

Macromolecular Bioscience

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The escalating rise in the population of multidrug‐resistant (MDR) pathogens coupled with their biofilm forming ability has struck the global health as nightmare. Alongwith the threat of aforementioned menace, the sluggish development of new antibiotics and the continuous deterioration of the antibiotic pipeline has stimulated the scientific community toward the search of smart and innovative alternatives. In near future, membrane targeting antimicrobial polymers, inspired from antimicrobial peptides, can stand out significantly to combat against the MDR superbugs. Many of these amphiphilic polymers can form nanoaggregates through self‐assembly with superior and selective antimicrobial efficacy. Additionally, these macromolecular nanoaggregrates can be utilized to engineer smart antibiotic‐delivery system for on‐demand drug‐release, exploiting the infection site's micoenvironment. This strategy substantially increases the local concentration of antibiotics and reduces the associated off‐target toxicity. Furthermore, amphiphilc macromolecules can be utilized to rejuvinate obsolete antibiotics to tackle the drug‐resistant infections. This review article highlights the recent developments in macromolecular architecture to design numerous nanostructures with broad‐spectrum antimicrobial activity, their application in fabricating smart drug delivery systems and their efficacy as antibiotic adjuvants to circumvent antimicrobial resistance. Finally, the current challenges and future prospects are briefly discussed for further exploration and their practical application in clinical settings.

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Approaches for the inhibition and elimination of microbial biofilms using macromolecular agents

Abstract: Macromolecular entities, such as polymers, peptides, proteins, and hybrid materials thereof, are herein reviewed as promising antibiofilm agents.

Cited by 108 publications

References 208 publications

Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms

Synergistic Antibiofilm Efficacy of Thymol and Piperine in Combination with Three Aminoglycoside Antibiotics against Klebsiella pneumoniae Biofilms

Research Progress on Cell Membrane-Coated Biomimetic Delivery Systems

Macromolecular Nanotherapeutics and Antibiotic Adjuvants to Tackle Bacterial and Fungal Infections

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