Approaches to Minimize Variation of Transgene Expression in Plants

Butaye, Katleen M.J.; Cammue, Bruno P. A.; Delauré, Stijn L.; Bolle, Miguel F.C. De

doi:10.1007/s11032-005-4929-9

Cited by 176 publications

(126 citation statements)

References 127 publications

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“…There are many factors that could be implicated for the incomplete penetrance of parthenogenesis within the original T 0 lines and T 1 offspring of lines g3f and g52. Variation of transgene expression from independent lines produced using the same transgene construct has been widely reported and summarized (15). Factors affecting transgene expression levels include transgene copy number, the complexity of integration patterns, and transgene integration sites.…”

Section: Discussionmentioning

confidence: 99%

A parthenogenesis gene of apomict origin elicits embryo formation from unfertilized eggs in a sexual plant

Conner

Mookkan

Huo

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

168

144

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Apomixis is a naturally occurring mode of asexual reproduction in flowering plants that results in seed formation without the involvement of meiosis or fertilization of the egg. Seeds formed on an apomictic plant contain offspring genetically identical to the maternal plant. Apomixis has significant potential for preserving hybrid vigor from one generation to the next in highly productive crop plant genotypes. Apomictic Pennisetum/Cenchrus species, members of the Poaceae (grass) family, reproduce by apospory. Apospory is characterized by apomeiosis, the formation of unreduced embryo sacs derived from nucellar cells of the ovary and, by parthenogenesis, the development of the unreduced egg into an embryo without fertilization. In Pennisetum squamulatum (L.) R.Br., apospory segregates as a single dominant locus, the aposporyspecific genomic region (ASGR). In this study, we demonstrate that the PsASGR-BABY BOOM-like (PsASGR-BBML) gene is expressed in egg cells before fertilization and can induce parthenogenesis and the production of haploid offspring in transgenic sexual pearl millet. A reduction of PsASGR-BBML expression in apomictic F 1 RNAi transgenic plants results in fewer visible parthenogenetic embryos and a reduction of embryo cell number compared with controls. Our results endorse a key role for PsASGR-BBML in parthenogenesis and a newly discovered role for a member of the BBM-like clade of APETALA 2 transcription factors. Induction of parthenogenesis by PsASGR-BBML will be valuable for installing parthenogenesis to synthesize apomixis in crops and will have further application for haploid induction to rapidly obtain homozygous lines for breeding.apomixis | parthenogenesis | AP2 transcription factor | BABY BOOM | Pennisetum

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Section: Discussionmentioning

confidence: 99%

A parthenogenesis gene of apomict origin elicits embryo formation from unfertilized eggs in a sexual plant

Conner

Mookkan

Huo

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

168

144

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“…In that case, it is difficult to obtain homogenic expression data. It has been described in different studies that lines with a desired level of transgene expression have lost the transgenic trait in subsequent generations (Butaye et al 2005). Similar problems could be expected for later stages of tree growth and development.…”

mentioning

confidence: 78%

“…Thereby the TGS could be mediated by surrounding heterochromatin, by endogenous repetitive sequences, by transgene-genomic junctions, by (trans)gene repeats, by aberrant promoter transcripts and by DNA viruses (summarized by Fagard and Vaucheret, 2000). Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism of eukaryotes which defends host cells against viruses, protects the genome from transposons and regulates gene expression (Butaye et al 2005). PTGS was often found in lines with methylation of the transcribed region of the transgene.…”

mentioning

confidence: 99%

Evaluation of the uniformity and stability of T-DNA integration and gene expression in transgenic apple plants

Flachowsky¹,

Riedel²,

Reim³

et al. 2008

Electron. J. Biotechnol.

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The generation of transgenic apple plants relies on the molecular analysis of transgene integration and expression based on polymerase chain reaction (PCR) analysis, blotting techniques and enzymatic assays on vitro leaves of putative transgenic regenerates. In order to assess the uniformity and the stability of transfer DNA (T-DNA) integration and gene expression, we studied 26 transgenic apple lines carrying the attacin E

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“…Leaf tissues from two transgenic plants containing GCC3X promoter were not induced by either SA or MeJ. It could be due to position effect of the transgene (GCC3X+SgfpS65T+Nos T) or various transcriptional/post-transcription gene silencing mechanisms (Bhat and Srinivasan, 2002;Butaye et al, 2005). Homology-dependent gene silencing (HDGS) is of great concern in plant genetic engineering strategies and is thought to be caused by multiple copies of homologous transgene and promoter sequences (Matzke et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Synthetic Promoters Containing Pathogen-Responsive <i>cis</i>-Elements

Yeri¹,

Bhat²,

Kuruvinashetti³

2013

mpb

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An attempt was made to study four synthetic promoters containing pathogen-responsive cis-elements with -46 region minimal promoter of CaMV 35S for their inducibility in tobacco upon exposure to salicylic acid (SA) and methyl jasmonate (MeJ). On 15 th day of establishment, none of the untreated plants, except those carrying CaMV 35S-driven SgfpS65T showed reporter expression. Plants sprayed with 5 mM SA or MeJ showed SgfpS65T expression after 5 min, 1 h, 2 h, 3 h and 7 h. GCC2X and S2X were induced by both SA and MeJ, W2X was induced by SA, but not by MeJ. However, GCC3X promoter did not show any induction. With SA, the strength of induction of W2X and GCC2X promoters as measured by ImageJ software were comparable and relatively higher than S2X. Similarly, GCC2X and S2X had similar magnitude of induction with MeJ. In general, the synthetic promoters were more strongly induced by SA compared to MeJ, however their strength was less compared to that of CaMV 35S promoter

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Approaches to Minimize Variation of Transgene Expression in Plants

Cited by 176 publications

References 127 publications

A parthenogenesis gene of apomict origin elicits embryo formation from unfertilized eggs in a sexual plant

A parthenogenesis gene of apomict origin elicits embryo formation from unfertilized eggs in a sexual plant

Evaluation of the uniformity and stability of T-DNA integration and gene expression in transgenic apple plants

Functional Analysis of Synthetic Promoters Containing Pathogen-Responsive <i>cis</i>-Elements

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