Appropriate Interferon-alpha Therapy for Chronic Hepatitis C: An Assessment by Quantitative Changes in Serum Hepatitis C Virus-RNA.

Watanabe, Shozo; Kobayashi, Yoshinao; Konishi, Masayoshi; Yokoi, Masato; Kakehashi, Ryuichi; Kaito, Masahiko; Suzuki, Shiro

doi:10.2169/internalmedicine.32.523

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…An initial step at 50°C for 30’ was carried out to activate Superscript, followed by 2’ at 94°C and 35 cycles of PCR (15”at 94°C, 30” at 50°C, 30” at 72°C), with a final extension step at 72°C for 7’. Ten μl of the produced amplicons were subjected to a nested PCR in a volume of 50 μl containing 10X PCR Gold Buffer, 25mM MgCl 2 , dNTP 10mM, 1.25 U Taq Gold (Roche Molecular System Inc, Branchburg, New Jersey, USA), and 0.2 μM of primers HCV3 ( ) and HCV4 ( ) [ 69 ].…”

Section: Methodsmentioning

confidence: 99%

HCV replication in gastrointestinal mucosa: Potential extra-hepatic viral reservoir and possible role in HCV infection recurrence after liver transplantation

et al. 2017

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PurposeHepatitis C virus (HCV) predominantly infects hepatocytes, although it is known that receptors for viral entry are distributed on a wide array of target cells. Chronic HCV infection is indeed characterized by multiple non-liver manifestations, suggesting a more complex HCV tropism extended to extrahepatic tissues and remains to be fully elucidated. In this study, we investigated the gastrointestinal mucosa (GIM) as a potential extrahepatic viral replication site and its contribution to HCV recurrence.MethodsWe analyzed GIM biopsies from a cohort of 76 patients, 11 of which were HCV-negative and 65 HCV-positive. Of these, 54 biopsies were from liver-transplanted patients. In 29 cases, we were able to investigate gastrointestinal biopsies from the same patient before and after transplant. To evaluate the presence of HCV, we looked for viral antigens and genome RNA, whilst to assess viral replicative activity, we searched for the replicative intermediate minus-strand RNA. We studied the genetic diversity and the phylogenetic relationship of HCV quasispecies from plasma, liver and gastrointestinal mucosa of HCV-liver-transplanted patients in order to assess HCV compartmentalization and possible contribution of gastrointestinal variants to liver re-infection after transplantation.ResultsHere we show that HCV infects and replicates in the cells of the GIM and that the favorite hosts were mostly enteroendocrine cells. Interestingly, we observed compartmentalization of the HCV quasispecies present in the gastrointestinal mucosa compared to other tissues of the same patient. Moreover, the phylogenetic analysis revealed a high similarity between HCV variants detected in gastrointestinal mucosa and those present in the re-infected graft.ConclusionsOur results demonstrated that the gastrointestinal mucosa might be considered as an extrahepatic reservoir of HCV and that could contribute to viral recurrence. Moreover, the finding that HCV infects and replicates in neuroendocrine cells opens new perspectives on the role of these cells in the natural history of HCV infection.

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Section: Methodsmentioning

confidence: 99%

HCV replication in gastrointestinal mucosa: Potential extra-hepatic viral reservoir and possible role in HCV infection recurrence after liver transplantation

et al. 2017

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“…The response to interferon also correlates with viral load [22]. There are two commer cial tests for quantitative HCV RNA determination.…”

Section: Molecular Biology> Tools To Detect Hepatitis C Viremiamentioning

confidence: 99%

Hepatitis C Virus Infection: Diagnosis, Natural Course and Therapy

Tillmann

Manns²

1996

Kidney Blood Press Res

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The hepatitis C virus is a 9.4-kb single-stranded positive RNA virus. After infection, more than 80% of patients develop a chronic carrier state. The diagnosis is based on the detection of hepatitis C virus antibodies and of HCV RNA. Recently, methods have been established to quantify HCV RNA levels and determine HCV genotypes. Interferon treatment gives long-term response in 10-20% of individuals. Low transaminase levels, low levels of viremia and low histological activity are positive predictive parameters for treatment response. In contrast, cirrhosis, high ferritin levels and associated markers of autoimmunity are negative predictors of treatment response. At present, the combination of interferon with the nucleoside analogue ribavirin is being evaluated. Endstages of hepatitis-C-induced cirrhosis are treated with liver transplantation. Reinfection of the graft occurs regularly, the clinical implications of which are not yet clear. Our knowledge of the spontaneous course and treatment response of hepatitis C infection in hemodialysis patients is limited.

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Chronic hepatitis C and interferon alpha: conventional and cumulative meta-analyses of randomized controlled trials

Cammà¹,

Pinzello

Morabito³

et al. 1999

The American Journal of Gastroenterology

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Appropriate Interferon-alpha Therapy for Chronic Hepatitis C: An Assessment by Quantitative Changes in Serum Hepatitis C Virus-RNA.

Cited by 8 publications

References 12 publications

HCV replication in gastrointestinal mucosa: Potential extra-hepatic viral reservoir and possible role in HCV infection recurrence after liver transplantation

HCV replication in gastrointestinal mucosa: Potential extra-hepatic viral reservoir and possible role in HCV infection recurrence after liver transplantation

Hepatitis C Virus Infection: Diagnosis, Natural Course and Therapy

Chronic hepatitis C and interferon alpha: conventional and cumulative meta-analyses of randomized controlled trials

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