Appropriate nonwoven filters effectively capture human peripheral blood cells and mesenchymal stem cells, which show enhanced production of growth factors

Abstract

Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2-22 lm fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2-1.8 lm captured over 70 % of leukocytes or 90 % of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor b1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.

A novel cell-containing device for regenerative medicine: biodegradable nonwoven filters with peripheral blood cells promote wound healing

“…There is a possibility that such enriched TGF-b1 may suppress excessive inflammation and promote granulation tissue with angiogenesis. This significant enhancement of TGF-b1 production by nonwoven PLA filters was also observed in h-PBCs [1]. The production of these two growth factors, FGF-7 and TGF-b1, at the wound sites might have some contribution to the acceleration of wound healing.…”

“…Contrary to m-PBCs, the VEGF production of h-PBCs was enhanced by capture on PLA filters [1]. Although the reasons for this difference may be due to species difference of the response to the filter materials or the difference in the cell size when contacted with the same nonwoven filters, more detailed study is necessary.…”

“…The nonwoven filter devices for capturing cells were prepared according to the method described previously [1]. Briefly, nonwoven PLA filter disks that were 13 mm in diameter were washed with water, sterilized with 70 % ethanol, and dried in a laminar flow cabinet.…”

“…The device consists of nonwoven filters that effectively capture human peripheral blood cells (h-PBCs) and mesenchymal stem cells [1]. The h-PBCs captured on the nonwoven filters enhanced their production of growth factors, such as vascular endothelial growth factor (VEGF), transforming growth factor b1 (TGF-b1), and plateletderived growth factor AB (PDGF-AB) [1]. The cells captured on the filters are expected to serve as a local supply of growth factors in a time-dependent manner for tissue repairs, including wound healing.…”

Combination of Nonwoven Filters and Mesenchymal Stem Cells Reduced Glomerulosclerotic Lesions in Rat Chronic Kidney Disease Models

“…In our previous studies, nonwoven filter disks composed of polylactic acid (PLA) fibers with fiber diameters of 1.8 µm effectively captured MSCs and peripheral blood cells and enhanced the production of several growth factors. Specifically, human peripheral blood cells captured on nonwoven biodegradable PLA filters enhanced the production of VEGF, platelet-derived growth factor-AB, and transforming growth factor (TGF)-β1; furthermore, human MSCs captured on nonwoven biodegradable PLA filters enhanced the production of VEGF [13]. Further, as a therapeutic device, nonwoven filters that captured cells were used to enhance wound healing [14].…”