Approved Laboratory Technic

Kolmer, John A.

doi:10.1097/00007611-195203000-00027

Cited by 45 publications

(51 citation statements)

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“…The seed powder was analyzed for the crude protein, minerals, calcium and phosphorus as per the standard protocol described in AOAC (1995). The trace minerals, zinc and copper were analysed in 1.0 g plant sample in polarized Zeeman atomic absorption spectrophotometer (Z-2300, HITACHI) as per standard method described Kolmer et al (1951). The aqueous extract was prepared using standard protocol (Singh et al 2012).…”

Section: Methodsmentioning

confidence: 99%

Anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against Haemonchus contortus of small ruminants

Singh¹,

Singh²,

Verma³

et al. 2014

J Parasit Dis

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Haemonchus contortus infection is the major hurdle in growth and productivity in small ruminants especially in tropical countries. Indiscriminate and long term use of currently available chemotherapeutic agents lead to development of resistance in microorganisms and residual problems in population. Therefore, present study was undertaken to evaluate the anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against adult H. contortus worms of small ruminants. Proximate analysis of seed powder shows high crude protein, Cu, Zn and Ca concentration. Phytochemical ingredients of extract revealed high total phenolic, flavonoids and tannin contents. In vitro experimental trial revealed complete mortality of H. contortus worms at the concentration of 100 mg/ml at the time exposure of 8 h. At 50 mg/ml concentration the mortality at 6 and 8 h were 45.45 ± 4.55 and 63.64 ± 4.54 %. Median lethal concentration (LC 50 ) at 6 and 8 h were 59.90 and 28.92 mg/ ml, respectively. Levamisole at concentration of 0.5 mg/ml caused 50 % mortality at 2 h post exposure and 100 % mortality at 4 h post exposure. The results confirm the cidal effect of the aqueous extract of Z. armatum DC. seeds on adult H. contortus worms.

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Section: Methodsmentioning

confidence: 99%

Anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against Haemonchus contortus of small ruminants

Singh¹,

Singh²,

Verma³

et al. 2014

J Parasit Dis

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“…In situ hybridizations were performed using a Spi-1/PU.1 antisense mRNA probe, controlled by the Spi-1/PU.1 sense mRNA probe. Pappenheim coloration was used as mean to identify cell types in the hematopoietic organs (Kolmer and Boerner, 1945). Figure 4a showed a high expression of the Spi-1/PU.1 mRNAs in the bursa of Fabricius whereas sense mRNA probe revealed distinct highly refringent areas (Figure 4c).…”

Section: Expression Pattern Of Chicken Spi-1 Mrnasmentioning

confidence: 99%

Isolation and characterization of a chicken homologue of the Spi-1/PU.1 transcription factor

et al. 1998

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“…The contents of the beaker were heated until the final volume was once again reduced to 0.5 ml. The digested samples were cooled and diluted to 10 ml with penta-distilled water (Kolmer et al 1951). Lead analysis was carried out in Atomic Absorption Spectrophotometer (model AAS 4129; Electronic Corporation of India).…”

Section: Methodsmentioning

confidence: 99%

Beneficial Effects of Aluminum on the Progression of Lead‐Induced Nephropathy in Rats

Shakoor

Gupta

Singh

et al. 2000

Pharmacology & Toxicology

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Abstract:We studied the effect of aluminum on lead-induced nephropathy in male albino rats. Five groups of male albino rats were given either water only or lead acetate (125 mg/kg body weight) and/or aluminum chloride (50 mg/kg body weight or 100 mg/kg body weight) for a period of 90 days. Aluminum was found to prevent the lead-induced increase in the relative organ (kidney) weight in a dose-dependent manner. Aluminum also prevented lead-induced increase in plasma creatinine levels of the treated animals. Estimation of lead concentration in kidneys of different treatment groups revealed that the net deposition of lead was lower in animals which were given both lead acetate and aluminum chloride simultaneously. The results showed that aluminum offers some protection against lead-induced nephrotoxicity in a time-and dose-dependent manner.

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Approved Laboratory Technic

Cited by 45 publications

References 0 publications

Anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against Haemonchus contortus of small ruminants

Anthelmintic efficacy of aqueous extract of Zanthoxylum armatum DC. seeds against Haemonchus contortus of small ruminants

Isolation and characterization of a chicken homologue of the Spi-1/PU.1 transcription factor

Beneficial Effects of Aluminum on the Progression of Lead‐Induced Nephropathy in Rats

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