Aptamer affinity chromatography:

Romig, Timothy S; Bell, Carol; Drolet, Daniel

doi:10.1016/s0378-4347(99)00243-1

Cited by 172 publications

(61 citation statements)

References 41 publications

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“…Aptamers have been used in flow cytometry [9,10], biosensors [11,12], affinity probe capillary electrophoresis [13], capillary electrochromatography [14,15], and affinity chromatography [16,17]. However, this discussion has been limited to those examples which led to the incorporation of aptamers as stationary phases and those in which aptameric stationary phases were utilized in electrophoretic and chromatographic separations.…”

Section: Applications In Analytical Chemistrymentioning

confidence: 99%

Aptamers as analytical reagents

2002

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Many important analytical methods are based on molecular recognition. Aptamers are oligonucleotides that exhibit molecular recognition; they are capable of specifically binding a target molecule, and have exhibited affinity for several classes of molecules. The use of aptamers as tools in analytical chemistry is on the rise due to the development of the "systematic evolution of ligands by exponential enrichment" (SELEX) procedure. This technique allows high-affinity aptamers to be isolated and amplified when starting from a large pool of oligonucleotide sequences. These molecules have been used in flow cytometry, biosensors, affinity probe electrophoresis, capillary electrochromatography, and affinity chromatography. In this paper, we will discuss applications of aptamers which have led to the development of aptamers as chromatographic stationary phases and applications of these stationary phases; and look towards future work which may benefit from the use of aptamers as stationary phases.

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Section: Applications In Analytical Chemistrymentioning

confidence: 99%

Aptamers as analytical reagents

2002

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“…Because of the specificity of the aptamer, undesirable solutes tend to have no affinity for the immobilized ligand. An elution buffer is then applied to dissociate the retained target from its binding site [40,48,49]. In a CE assay, the analysis of preincubated targetaptamer mixtures is the approach especially useful for quantitative measurements of analytes that form with high affinity complex with the ligand.…”

Section: Type Of Assays In Lc and Cementioning

confidence: 99%

Nucleic acid aptamer molecular recognition principles and application in liquid chromatography and capillary electrophoresis

Peyrin¹

2009

J of Separation Science

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Nucleic acid aptamers isolated from the systematic evolution of ligands by exponential enrichment (SELEX) have the capacity to recognize various classes of target molecules with high affinity and specificity. In this context, the development of aptamer-based molecular recognition tools has become a very interesting and promising analytical strategy during the last few years. In this review, the molecular recognition features of aptamers as well as the key factors for their practical applicability to the chromatographic and capillary electrophoretic fields are summarized.

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“…Aptamers have been shown to be promising tools in a wide field of diagnostic and analytical applications, including affinity chromatography, capillary electrophoresis, proximity-ligation assays with subsequent aptamer quantification by real-time PCR, Western-blot, enzymelinked sandwich assays (ELISA), flow cytometry, and in vivo cancer cell imaging [22][23][24][25][26][27][28]. In a biosensor assay for thrombin detection, aptamers worked just as well as antibodies [29].…”

Section: Aptamers As Tools For Target Analysis and Separationmentioning

confidence: 99%

Recognition of biomarkers and cell‐specific molecular signatures: Aptamers as capture agents

Nery

Wrenger

Ulrich³

2009

J of Separation Science

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RNA and DNA aptamers developed by systematic evolution of ligands by exponential enrichment (SELEX) have turned into important tools in diagnostics, research, and therapeutics. Unlike antibodies, high-affinity and specific aptamers identified through an in vitro selection process can be chemically modified to gain nuclease resistances in biological fluids and to extend their bioavailability in animals. Aptamers can be raised against virtually any target including those which are toxic or do not elicit any immune response in animals. They can be developed in automated processes against various protein targets and then easily modified by attaching fluorescence reporters, nanoparticles or biotin moieties, rival antibodies in high-throughput proteomics and cell separations. In this review, we will discuss the high competence of aptamers in recognizing biomarkers and molecular signatures of cell surfaces, and how these unique features can be exploited for the identification and isolation of cancer, stem cells and even detection of parasite-infected cells.

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Aptamer affinity chromatography:

Cited by 172 publications

References 41 publications

Aptamers as analytical reagents

Aptamers as analytical reagents

Nucleic acid aptamer molecular recognition principles and application in liquid chromatography and capillary electrophoresis

Recognition of biomarkers and cell‐specific molecular signatures: Aptamers as capture agents

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