Aptamer and gold nanorod–based fumonisin B1 assay using both fluorometry and SERS

He, Deyun; Wu, Zhengzong; Cui, Bo; Xu, Enbo

doi:10.1007/s00604-020-4192-0

Cited by 44 publications

(28 citation statements)

References 23 publications

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“…So far, two aptamers composed by 96 and 80 nucleotides, have been reported through SELEX and utilized in different biosensing approaches [148,149], all the aptamer-based sensors are chronologically described in Table 5, while the binding and functionalization conditions are illustrated in Table 6. From the 31 aptasensors found in the literature, 24 utilized the 96 nt aptamer [148], one method applied a shortened version (60 nt) from this first sequence [159], one platform included the second 80 nt aptamer [156], two biosensors manipulated a condensed version (40 nt) of the second main aptamer [164,167], and three references did not specify their single-stranded (ss) DNA sequence [175,177,198]. The schematic representation of each type of aptasensor assay is illustrated in Fig 6a a for the biosensors involving the initial 96 nt aptamer, and in Fig 6b for the application of the subsequent and not specified aptamers.…”

Section: Other Methodsmentioning

confidence: 99%

“…Three studies published by the same research group did not specify the aptamers sequence for the detection of FB1. The first approach relied on the hybridization of Cy5.5-aptamer and its cDNA on gold nanorods, with a further measurement of their SERS (LOD: 0.0003 µg/L) and fluorescent (LOD:0.0005 µg/L) signals under the presence of the target mycotoxin [175]. The second work, which so far is the most sensitive aptasensor for FB1, was reported with a LOD of 0.000003 µg/L.…”

Section: Not Specified Sequences and Alternative Methodsmentioning

confidence: 99%

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Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida

Gong²,

Goycoolea³

2020

Preprint

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Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

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Section: Other Methodsmentioning

confidence: 99%

Section: Not Specified Sequences and Alternative Methodsmentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida

Gong²,

Goycoolea³

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…and inorganic nanomaterials (graphene, [34][35][36] boron nitride, [37][38][39] semiconductor, [40][41][42] etc.). The metal substrates have been shaped into spheres, [43,44] rods, [45][46][47] stars, [48][49][50][51] internal nanogaps, [52,53] and so on. As a permutation and combination of the former two terms, gold nanostar is more adopted one on account of the following reasons: Gold has the advantages of chemical inertness, shape and size controllability, and good biocompatibility.…”

Section: Design Of Responsive Sers Probesmentioning

confidence: 99%

Emergence of Responsive Surface‐Enhanced Raman Scattering Probes for Imaging Tumor‐Associated Metabolites

Yin

Jin

Duan

et al. 2022

Adv Healthcare Materials

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As a core hallmark of cancer, metabolic reprogramming alters the metabolic networks of cancer cells to meet their insatiable appetite for energy and nutrient. Tumor-associated metabolites, the products of metabolic reprogramming, are valuable in evaluating tumor occurrence and progress timely and accurately because their concentration variations usually happen earlier than the aberrances demonstrated in tissue structure and function. As an optical spectroscopic technique, surface-enhanced Raman scattering (SERS) offers advantages in imaging tumor-associated metabolites, including ultrahigh sensitivity, high specificity, multiplexing capacity, and uncompromised signal intensity. This review first highlights recent advances in the development of stimuli-responsive SERS probes. Then the mechanisms leading to the responsive SERS signal triggered by tumor metabolites are summarized. Furthermore, biomedical applications of these responsive SERS probes, such as the image-guided tumor surgery and liquid biopsy examination for tumor molecular typing, are summarized. Finally, the challenges and prospects of the responsive SERS probes for clinical translation are also discussed.

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“…On the other hand, the lack of highly affinitive, specific, and reliable aptamers for other mycotoxins becomes a major hindrance to expanding the scope of targets. A new study made breakthrough on this aspect by developing a SERS and fluorescence dual detection of FB 1 (D. He et al., 2020). In a more recent work, they applied a similar strategy for simultaneous detection of ZEN, OTA, and FB 1 by constructing an AuNP–upconversion nanoparticle (UCNP)–AuNP trimer (Z. Wu et al., 2020).…”

Section: Sers Detection With Specific Recognitionmentioning

confidence: 99%

Recent progress in mycotoxins detection based on surface‐enhanced Raman spectroscopy

Zhai

You

Ouyang

et al. 2021

Comp Rev Food Sci Food Safe

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Mycotoxins are toxic compounds naturally produced by certain types of fungi. The contamination of mycotoxins can occur on numerous foodstuffs, including cereals, nuts, fruits, and spices, and pose a major threat to humans and animals by causing acute and chronic toxic effects. In this regard, reliable techniques for accurate and sensitive detection of mycotoxins in agricultural products and food samples are urgently needed. As an advanced analytical tool, surface‐enhanced Raman spectroscopy (SERS), presents several major advantages, such as ultrahigh sensitivity, rapid detection, fingerprint‐type information, and miniaturized equipment. Benefiting from these merits, rapid growth has been observed under the topic of SERS‐based mycotoxin detection. This review provides a comprehensive overview of the recent achievements in this area. The progress of SERS‐based label‐free detection, aptasensor, and immunosensor, as well as SERS combined with other techniques, has been summarized, and in‐depth discussion of the remaining challenges has been provided, in order to inspire future development of translating the techniques invented in scientific laboratories into easy‐to‐operate analytic platforms for rapid detection of mycotoxins.

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Aptamer and gold nanorod–based fumonisin B1 assay using both fluorometry and SERS

Cited by 44 publications

References 23 publications

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Emergence of Responsive Surface‐Enhanced Raman Scattering Probes for Imaging Tumor‐Associated Metabolites

Recent progress in mycotoxins detection based on surface‐enhanced Raman spectroscopy

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