Aptamer-based biosensors for the diagnosis of sepsis

Liu, Lubin; Han, Zeyu; An, Fei; Gong, Xuening; Zhao, Chenguang; Zheng, Wei; Mei, Li; Zhou, Qihui

doi:10.1186/s12951-021-00959-5

Cited by 37 publications

(19 citation statements)

References 124 publications

(171 reference statements)

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“…In this decade, carbon-based nanomaterials have been used as antibacterial materials owing to their excellent physicochemical properties and good biocompatibility [ 10 – 12 ]. Particularly, carbon dots (CDs), a new member of the carbon-based material family, have gained growing attention since they were accidentally discovered in 2004 [ 13 – 15 ]. CDs with small size, water-solubility, photoluminescence property, low toxicity, and high biocompatibility, possess a wide range of applications in bioimaging, biosensors, detection probes, and drug delivery [ 16 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

et al. 2022

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Enterococcus faecalis (E. faecalis) biofilm-associated persistent endodontic infections (PEIs) are one of the most common tooth lesions, causing chronic periapical periodontitis, root resorption, and even tooth loss. Clinical root canal disinfectants have the risk of damaging soft tissues (e.g., mucosa and tongue) and teeth in the oral cavity, unsatisfactory to the therapy of PEIs. Nanomaterials with remarkable antibacterial properties and good biocompatibility have been developed as a promising strategy for removing pathogenic bacteria and related biofilm. Herein, carbon dots (CDs) derived from fucoidan (FD) are prepared through a one-pot hydrothermal method for the treatment of PEIs. The prepared FDCDs (7.15 nm) with sulfate groups and fluorescence property are well dispersed and stable in water. Further, it is found that in vitro FDCDs display excellent inhibiting effects on E. faecalis and its biofilm by inducing the formation of intracellular and extracellular reactive oxygen species and altering bacterial permeability. Importantly, the FDCDs penetrated the root canals and dentinal tubules, removing located E. faecalis biofilm. Moreover, the cellular assays show that the developed FDCDs have satisfactory cytocompatibility and promote macrophage recruitment. Thus, the developed FDCDs hold great potential for the management of PEIs. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

et al. 2022

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“…Besides the biochemical properties, their biophysical structure can significantly mediate cell attachment, shape, viability, the differentiation or pluripotency of stem cells, and even tissue repair and regeneration Cui et al, 2020;Yu et al, 2020;Yu et al, 2021;Zhou et al, 2020;Liu et al, 2021;Yang et al, 2021a;Yang et al, 2021b). Recently, the development of nanofibrous materials has received increasing attention in tissue engineering and regenerative medicine due to their outstanding properties, such as their favorable biological properties, sufficient mechanical strength, highly porous mesh with interconnectivity, extremely high specific surface area, and aspect ratio (Zhou et al, 2015;Zhou et al, 2017;Kenry and Lim, 2017;Xue et al, 2019;Ahmadi et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

Chen

Zhu

Hao

et al. 2021

Front. Bioeng. Biotechnol.

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Sulfated polysaccharide fucoidan (FD) is widely applied in biomedical applications owing to its outstanding bioactivities. In addition to the biochemical features, the architecture of biomaterials plays a critical role in tissue repair and regeneration. Particularly, nanofibers have elicited great interest due to their extracellular matrix-like structure, high specific surface area, and favorable biological properties. Herein, chitosan-modified FD/ultra-high molecular weight polyethylene oxide (UHMWPEO) nanofibers are developed via green electrospinning and electrostatic interaction for studying their interaction with endothelial cells. The appropriate solvent is screened to dissolve FD. The electrospinnability of FD/UHMWPEO aqueous solutions is greatly dependent on the weight ratios of FD/UHMWPEO. The incorporation of UHMWPEO significantly improves the electrospinnability of solution and thermo-stability of nanofibers. Also, it is found that there is good miscibility or no phase separation in FD/UHMWPEO solutions. In vitro biological experiments show that the chitosan-modified FD/UHMWPEO nanofibers greatly facilitate the adhesion of endothelial cells and inhibit the attachment of monocytes. Thus, the designed FD-based nanofibers are promising bio-scaffolds in building tissue-engineered blood vessels.

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“…We demonstrated multiplexed detection of DNA sequences with biotinylated oligonucleotide probes. Biotinylated peptides are, for example, potentially useful for the detection of protease activity (see SI Figure S13), and biotinylated aptamers, ,− small antibody derivatives, − and affinity proteins , may also be suitable for protein detection with this new cFRET configuration.…”

Section: Discussionmentioning

confidence: 99%

A Dendrimer-Based Time-Gated Concentric FRET Configuration for Multiplexed Sensing

Tsai

Algar

2022

ACS Nano

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Forster resonance energy transfer (FRET) is widely used for the development of biological probes and sensors. In this context, the norm for multiplexed detection is deployment of multiple probes, each a discrete donor−acceptor pair. Concentric FRET (cFRET) probes enable multiplexed sensing with a single vector but, to date, have only been developed around semiconductor quantum dots, which may limit the scope of biological applications for such probes. Here, we demonstrate that dendrimers labeled with a luminescent terbium complex (Tb) are a viable and advantageous alternative platform for cFRET probes. Polyamidoamine dendrimers were functionalized with Tb, biotin, NeutrAvidin, and three types of dye-labeled oligonucleotide probes to establish a network of competitive and sequential Tb-to-dye and dye-to-dye FRET pathways. These probes were characterized physically and photophysically, and a time-gated multiplexed assay for DNA targets was demonstrated. The time-gating offered by the Tb allowed the rejection of background autofluorescence from serum. More broadly, this dendrimer-based architecture shows that cFRET is a general concept and is an important step toward a new generation of probes for biological sensing.

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Aptamer-based biosensors for the diagnosis of sepsis

Cited by 37 publications

References 124 publications

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

A Dendrimer-Based Time-Gated Concentric FRET Configuration for Multiplexed Sensing

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