Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection

Wei, Yangdao; Tao, Zhenzhen; Lü, Wei; Zong, Chengli; Wu, Jiajia; Tan, Xiao; Wang, Buhua; Guo, Zixuan; Zhang, Ling; Yuan, Haoyu; Wang, Peng; Yang, Zhiqing; Wan, Yi

doi:10.1016/j.bios.2022.114282

Cited by 56 publications

(19 citation statements)

References 39 publications

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“…1A). The detection targets have also been expanded from nucleic acids to other analytes such as live bacterial cells, proteins and metal ions, [17][18][19][20][21] which have been summarized in Table 1. Moreover, because of eliminating the complicated target amplification process, the amplification-free CRISPR/Cas systems are capable of adapting to compact, portable, and easy-to-operate devices for promising field-deployable monitoring.…”

Section: Qinqin Humentioning

confidence: 99%

Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

et al. 2023

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Section: Qinqin Humentioning

confidence: 99%

Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

et al. 2023

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“…To date, there have been a number of advanced sensors using colorimetric, fluorescence, , Raman, and fluorescence polarization (FP) responses as output signals for the detection of pathogens to overcome problems associated with traditional microbiological detection techniques that are time- and labor-intensive. In particular, FP-based sensors permit fast homogeneous “mixing and reading” detection without the requirements of quenchers, donor–acceptor pairs, and sample separation. , The FP signal is insensitive to sample fluorescence fluctuations and photobleaching, so it is not surprising that there has been a great deal of interest in using the FP tool for biochemical analysis.…”

Section: Introductionmentioning

confidence: 99%

Self-Assembly of Multivalent Aptamer-Tethered DNA Monolayers Dedicated to a Fluorescence Polarization-Responsive Circular Isothermal Strand Displacement Amplification for Salmonella Assay

Zhang

Peng

et al. 2023

Anal. Chem.

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Pathogenic bacteria are pathogens widely spread that are capable of causing mild to life-threatening diseases in human beings or other organisms. Rationally organizing the simple helical motif of double-stranded DNA (dsDNA) tiles into designed ensemble structures with architecturally defined collective properties could lead to promising biosensing applications for pathogen detection. In this work, we facilely engineered multivalent hairpin aptamer probe-tethered DNA monolayers (MHAP-DNA monolayers) and applied them to build a fluorescence polarization-responsive circular isothermal strand displacement amplification (FP-CSDA) for Salmonella assay. In this system, the MHAP-DNA monolayers were constructed based on a dsDNA tile-directed selfassembly. A FAM-labeled reporting probe (RP FAM ) with an inherent low FP signal serves as the signaling unit. The presence of target Salmonella leads to the trapping of F RP FAM into the super DNA monolayers via a target-triggered CSDA to peel off the tethered hairpin-structured aptamer probes (HAPs) responsible for the binding of RP FAM . As a result, the FP signal of the FAM fluorophore can be remarkably amplified due to the recycling of target Salmonella and the capacity of structural DNA materials to strongly restrict the free rotation of the FAM fluorophore but without a fluorescence quenching effect. Experimental results demonstrate that the FP assay is able to detect Salmonella with a low limit of detection (LOD) of 7.2 × 10 0 CFU/mL and high specificity. As a proof-ofconcept study, we envision our study using DNA nanoarchitecture as the foundation to modulate CSDA-based FP assays, promising to open up a new avenue for disease diagnosis, food safety detection, and biochemical studies.

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“…Among various recognition elements ( e.g. , antibodies, 4,17 aptamers, 18–20 antimicrobial peptides, 21 etc . ), antibodies are the most commonly used due to their high selectivity and binding affinity.…”

Section: Introductionmentioning

confidence: 99%

“…The most crucial aspect in the construction of labelfree electrochemical sensors for the detection of pathogenic bacteria is the choice of suitable recognition elements and their efficient immobilization onto the electrode surface. Among various recognition elements (e.g., antibodies, 4,17 aptamers, [18][19][20] antimicrobial peptides, 21 etc. ), antibodies are the most commonly used due to their high selectivity and binding affinity.…”

Section: Introductionmentioning

confidence: 99%

Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film

Wang

Lin

Liu

et al. 2022

Analyst

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Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection

Cited by 56 publications

References 39 publications

Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

Self-Assembly of Multivalent Aptamer-Tethered DNA Monolayers Dedicated to a Fluorescence Polarization-Responsive Circular Isothermal Strand Displacement Amplification for Salmonella Assay

Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film

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