Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens

Moshref, Zahra Sadat; Jalali, Tahmineh; Rezaei Adriani, Razieh; Soltati, Elahe; Mousavi Gargari, Seyed Latif

doi:10.1016/j.heliyon.2023.e16458

Cited by 7 publications

(1 citation statement)

References 26 publications

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“…However, ELASA was considerably more convenient and less timeconsuming. Another diagnostic application of ELASA-based diagnosis is the detection of SARS-CoV-2 [119] and the detection of the NS1 protein of Dengue Virus Serotype 2 [120]. Diáz-Fernandez and Ferapontova [121] discussed the potential of simple and low-cost cellulase-linked ELASA as a prospective diagnostic tool for a rapid and accurate liquid biopsy detection of HER2 and numerous other proteins for which aptamers are available.…”

Section: Adoption Of Enzyme-linked Immunosorbent Assay (Elisa) Proced...mentioning

confidence: 99%

New Insights into Aptamers: An Alternative to Antibodies in the Detection of Molecular Biomarkers

Domsicova,

Korcekova,

Poturnayova

et al. 2024

IJMS

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Aptamers are short oligonucleotides with single-stranded regions or peptides that recently started to transform the field of diagnostics. Their unique ability to bind to specific target molecules with high affinity and specificity is at least comparable to many traditional biorecognition elements. Aptamers are synthetically produced, with a compact size that facilitates deeper tissue penetration and improved cellular targeting. Furthermore, they can be easily modified with various labels or functional groups, tailoring them for diverse applications. Even more uniquely, aptamers can be regenerated after use, making aptasensors a cost-effective and sustainable alternative compared to disposable biosensors. This review delves into the inherent properties of aptamers that make them advantageous in established diagnostic methods. Furthermore, we will examine some of the limitations of aptamers, such as the need to engage in bioinformatics procedures in order to understand the relationship between the structure of the aptamer and its binding abilities. The objective is to develop a targeted design for specific targets. We analyse the process of aptamer selection and design by exploring the current landscape of aptamer utilisation across various industries. Here, we illuminate the potential advantages and applications of aptamers in a range of diagnostic techniques, with a specific focus on quartz crystal microbalance (QCM) aptasensors and their integration into the well-established ELISA method. This review serves as a comprehensive resource, summarising the latest knowledge and applications of aptamers, particularly highlighting their potential to revolutionise diagnostic approaches.

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Section: Adoption Of Enzyme-linked Immunosorbent Assay (Elisa) Proced...mentioning

confidence: 99%

New Insights into Aptamers: An Alternative to Antibodies in the Detection of Molecular Biomarkers

Domsicova,

Korcekova,

Poturnayova

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

Process, advances, and perspectives of graphene oxide-SELEX for the development of aptamer molecular probes: A comprehensive review

Chen,

Yang,

Cui

et al. 2024

Analytica Chimica Acta

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Improving synthesis and binding affinities of nucleic acid aptamers and their therapeutics and diagnostic applications

Mili,

Bachu,

Kuri

et al. 2024

Biophysical Chemistry

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Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens

Cited by 7 publications

References 26 publications

New Insights into Aptamers: An Alternative to Antibodies in the Detection of Molecular Biomarkers

New Insights into Aptamers: An Alternative to Antibodies in the Detection of Molecular Biomarkers

Process, advances, and perspectives of graphene oxide-SELEX for the development of aptamer molecular probes: A comprehensive review

Improving synthesis and binding affinities of nucleic acid aptamers and their therapeutics and diagnostic applications

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