Aptamer‐based ellipsometric sensor for ultrasensitive determination of aminoglycoside group antibiotics from dairy products

Abstract

BACKGROUNDResidual antibiotics taken along with food consumed through the food chain are the main cause of the super‐bacteria and may damage organs such as liver and kidney. Therefore, monitoring residual antibiotic levels of products in the food chain is both important and a requirement. Maximum residual limits for kanamycin and neomycin are 150 ng mL−1 and 500 ng mL−1 respectively, which are challenging for most sensor platforms. In this paper, a novel method is presented for the determination of antibiotics residues in animal‐derived foods.RESULTSAptamer‐based kanamycin and neomycin biosensors based on the spectroscopic ellipsometer and the surface plasmon resonance‐enhanced total internal reflection ellipsometer methods as transducing element were developed. Detection limits of both sensor platforms were in the 0.1–1 nmol L−1 ranges, and the detection range was between the detection limit and 1000 nmol L−1.CONCLUSIONBoth ellipsometry‐based aptasensors can be used as an alternative to the existing enzyme‐linked immunosorbent assay‐based method in terms of assay time (10 min), detection limit (0.22 ng mL−1 for neomycin and 0.048 ng mL−1 for kanamycin), and detection range. © 2020 Society of Chemical Industry

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

“…Sensors that can detect groups of compounds are preferred for rapid on-site detection. Caglayan [109] designed an aptasensor-based elliptical polarized light sensor for the determination of AGs in dairy products. Kanamycin and neomycin were successfully detected with good sensitivity and minimum detection limits of 0.22 ng/mL and 0.048 ng/mL, respectively.…”

Amphiphilic Conjugated Polyelectrolyte-Based Sensing System for Visually Observable Detection of Neomycin with High Sensitivity

“…In addition, the European Union (EU) had established the corresponding maximum residue limits (MRLs) for neomycin (500 μg kg −1 of meat, 1500 μg kg −1 of milk) for consumer protection. 49 Therefore, our sensing system has the potential to be applied to judge if the neomycin exceeds MRL in the food.…”

“…Our system is more competitive with other reported systems for neomycin detection. ,− Moreover, the visually observable feature, the fluorescence color change, is also a distinctive advantage for this sensory system. In addition, the European Union (EU) had established the corresponding maximum residue limits (MRLs) for neomycin (500 μg kg –1 of meat, 1500 μg kg –1 of milk) for consumer protection . Therefore, our sensing system has the potential to be applied to judge if the neomycin exceeds MRL in the food.…”

Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies

“…Notably most small molecules bind aptamers only with the one-site binding configuration because there is no room for the aptamer to interact with a second molecule [60]. Therefore, many SPR aptasensors have been developed based on this direct strategy [61][62][63][64][65]. For example Wu et al and Ashley et al immobilized a biotin-modified aptamer probe on an avidin modified chip through streptavidin-biotin interaction for the detection of aflatoxin in vin egar and lysozyme in milk [66,67].…”