Aptamer-Based Lateral Flow Biosensor for Rapid Detection of Salivary Cortisol

Dalirirad, Shima; Han, Daewoo; Steckl, A. J.

doi:10.1021/acsomega.0c03223

Cited by 110 publications

(61 citation statements)

References 53 publications

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“…S21 ). We found reports of Pure·SAL™ used in lateral flow strips and other investigations ( Dalirirad et al, 2020 ; Sa et al, 2020 ), but not in isothermal amplification assays. Literature investigations of Pure·SAL™ suggest that it removes greater than 70% of mucinous material from saliva, which would act as an inhibitor to our amplification assay ( Khurshid et al, 2017 ).…”

Section: Discussioncontrasting

confidence: 45%

A paper-based colorimetric molecular test for SARS-CoV-2 in saliva

Davidson¹,

Wang²,

Maruthamuthu³

et al. 2021

Biosensors and Bioelectronics: X

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Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.

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Section: Discussioncontrasting

confidence: 45%

A paper-based colorimetric molecular test for SARS-CoV-2 in saliva

Davidson¹,

Wang²,

Maruthamuthu³

et al. 2021

Biosensors and Bioelectronics: X

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show abstract

“…To further evaluate the potential application and feasibility of Ag NCs-labelled PEC immunosensor, we examined cortisol in human saliva samples, which range from 0.6 to 12.85 ng mL −1 in healthy people [ 42 , 43 ]. Firstly, saliva from four volunteers was collected twice in the morning and afternoon and detected by this method and ELISA after certain sample treatment (see ESM for saliva collection in details).…”

Section: Resultsmentioning

confidence: 99%

Signal-on photoelectrochemical immunoassay for salivary cortisol based on silver nanoclusters-triggered ion-exchange reaction with CdS quantum dots

Luo

Gao

et al. 2022

Anal Bioanal Chem

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Nowadays, the epidemic, employment, and academic pressures are seriously affecting our physical and mental health. Herein, we designed a magneto-controlled photoelectrochemical immunosensor for noninvasive monitoring of salivary cortisol regarded as a pressure biomarker. A competitive immunoassay model was established by coupling bovine serum albumin-cortisol modified magnetic beads (MB-BSA-cortisol) with silver nanoclusters (Ag NCs)-labelled anti-cortisol antibody, and quantity analysis was operated by photoelectrochemical measurement of the CdS/Au electrode as an ion-exchange platform. Accompanying the formation of immune complexes, the carried Ag NCs were readily dissolved with nitric acid to produce abundant silver ions, which transferred to the electrode for ion-exchange reaction with CdS quantum dots to produce Ag 2 S, a new electron–hole capture site, leading to a decrease in the photocurrent intensity. The photocurrent signal gradually recovered with the increase of concentration of target cortisol, acquiring the signal-on mode competitive immunosensing system, which is propitious to the detection of small molecules. Within optimal conditions, this sensor had a satisfactory linear relationship in the range of 0.0001–100 ng mL −1 with favorable repeatability, specificity, and acceptable method accuracy. The detection limit was as low as 0.06 pg mL −1 . In addition, this strategy provided new thought for the test of other small-molecule analytes and immunosensor applied in the complex biological system. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-022-03893-z.

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“…The technology behind the design and operation of these developments is highly varied. For instance, several attempts propose the use of antibodies to improve the specificity of the test being able to identify quantities in the pM range [111][112][113][114]. Nevertheless, it should be considered that their inclusion increases the test cost, which could limit its widespread use.…”

Section: Current Trends and Future Perspectives In Enzymatic Methods For Salivary Biomarkers Detectionmentioning

confidence: 99%

Enzymatic Methods for Salivary Biomarkers Detection: Overview and Current Challenges

2021

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Early detection is a key factor in patient fate. Currently, multiple biomolecules have been recognized as biomarkers. Nevertheless, their identification is only the starting line on the way to their implementation in disease diagnosis. Although blood is the biofluid par excellence for the quantification of biomarkers, its extraction is uncomfortable and painful for many patients. In this sense, there is a gap in which saliva emerges as a non-invasive and valuable source of information, as it contains many of the biomarkers found in blood. Recent technological advances have made it possible to detect and quantify biomarkers in saliva samples. However, there are opportunity areas in terms of cost and complexity, which could be solved using simpler methodologies such as those based on enzymes. Many reviews have focused on presenting the state-of-the-art in identifying biomarkers in saliva samples. However, just a few of them provide critical analysis of technical elements for biomarker quantification in enzymatic methods for large-scale clinical applications. Thus, this review proposes enzymatic assays as a cost-effective alternative to overcome the limitations of current methods for the quantification of biomarkers in saliva, highlighting the technical and operational considerations necessary for sampling, method development, optimization, and validation.

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Aptamer-Based Lateral Flow Biosensor for Rapid Detection of Salivary Cortisol

Cited by 110 publications

References 53 publications

A paper-based colorimetric molecular test for SARS-CoV-2 in saliva

A paper-based colorimetric molecular test for SARS-CoV-2 in saliva

Signal-on photoelectrochemical immunoassay for salivary cortisol based on silver nanoclusters-triggered ion-exchange reaction with CdS quantum dots

Enzymatic Methods for Salivary Biomarkers Detection: Overview and Current Challenges

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