Aptamer-based microcantilever-array biosensor for ultra-sensitive and rapid detection of okadaic acid

Wang, Yu; Rao, Depeng; Wu, Xiaoping; Zhang, Qingchuan; Wu, Shangquan

doi:10.1016/j.microc.2020.105644

Cited by 20 publications

(13 citation statements)

References 39 publications

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“…To enhance the sensitivity, GNPs that do not affect the specific matching between silver ion and cytosine-DNA layer were introduced to improve resonance frequency shift. Furthermore, genetically modified cantilever sensors can also be used with other environmental pollutants such as okadaic acid (OA) [ 172 ] and in food security, such as with profenofous pesticide residues in vegetables [ 173 ] and the hepatic toxin microcystin–leucine–arginine (MC-LR) [ 174 ].…”

Section: Application Of Genetic-probe-modified Cantilevermentioning

confidence: 99%

A Genosensor Based on the Modification of a Microcantilever: A Review

et al. 2023

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When the free end of a microcantilever is modified by a genetic probe, this sensor can be used for a wider range of applications, such as for chemical analysis, biological testing, pharmaceutical screening, and environmental monitoring. In this paper, to clarify the preparation and detection process of a microcantilever sensor with genetic probe modification, the core procedures, such as probe immobilization, complementary hybridization, and signal extraction and processing, are combined and compared. Then, to reveal the microcantilever’s detection mechanism and analysis, the influencing factors of testing results, the theoretical research, including the deflection principle, the establishment and verification of a detection model, as well as environmental influencing factors are summarized. Next, to demonstrate the application results of the genetic-probe-modified sensors, based on the classification of detection targets, the application status of other substances except nucleic acid, virus, bacteria and cells is not introduced. Finally, by enumerating the application results of a genetic-probe-modified microcantilever combined with a microfluidic chip, the future development direction of this technology is surveyed. It is hoped that this review will contribute to the future design of a genetic-probe-modified microcantilever, with further exploration of the sensitive mechanism, optimization of the design and processing methods, expansion of the application fields, and promotion of practical application.

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Section: Application Of Genetic-probe-modified Cantilevermentioning

confidence: 99%

A Genosensor Based on the Modification of a Microcantilever: A Review

et al. 2023

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“…[38] Chemical or biological recognition elements, usually nucleic acid aptamers, [40,41] antibodies, [7,42,43] enzymes, [14,44] and microorganisms, [8,24] can be used to capture target molecules with high sensitivity and specificity. Nucleic acid aptamers, abbreviated to aptamers, are usually DNA or RNA oligonucleotide fragments with a strong affinity for the target that are selected from the DNA molecule library by in vitro screening technology-Systematic Evolution of Ligands by Exponential Enrichment (SELEX), [45,46] as shown in Figure 1a. Compared to standard and mature antibodies, aptamers have the advantages of convenient screening, high stability, easy preparation, low molecular weight, simple modification groups, and wide range of targets.…”

Section: Introductionmentioning

confidence: 99%

“…At present, these sensors have experienced highspeed developments, and researchers have made full use of the superior properties of aptamers to achieve the detection of a large number of target analytes ranging from organic molecules over proteins and hormones to complex structures like microorganisms or cells. [49][50][51][52] Meanwhile, some excellent reviews of aptamer biochemical sensing have been proposed, [45][46][47][48]50,53,54] focusing on the detections of different analytes and the applica-tions of aptasensors in different fields. For example, in 2020, Qi et al summarized the research progress of split aptamer (SPA) in small molecule detection.…”

Section: Introductionmentioning

confidence: 99%

Advances in Optical Fiber Aptasensor for Biochemical Sensing Applications

Zhang

et al. 2023

Adv Materials Technologies

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Optical fiber biochemical sensing technology, as a cutting‐edge, multidisciplinary, and universal sensing technology, is gradually becoming the research focus in the field of biochemical sensing. They enjoy distinctive characteristics such as high cost‐effectiveness, flexibility, resistance to electromagnetic interference, and small size and can be used to detect biochemical analytes at ultra‐low concentrations with ultra‐low sample consumption, even when these biochemical analytes are in hard‐to‐reach environments. An aptamer is a DNA or RNA oligonucleotide fragment with a high affinity for the target selected from random sequences. Due to their advantages of simple synthesis, low cost, good stability, good specificity, and strong affinity, they are gradually becoming ideal recognition elements and may replace antibodies as next‐generation target receptors. Therefore, aptamers, as functional materials, are integrated into optical fiber sensors, which are called optical fiber aptasensors, providing new opportunities for the construction of high‐performance and intelligent biochemical sensors. This article aims to review the recent research progress of fiber‐optic aptamer biochemical sensors, with a focus on their sensing mechanisms and applications. Finally, based on the existing deficiencies of fiber‐optic aptamer biochemical sensors and practical requirements, their future development trends are briefly discussed.

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“…[24] It has rapidly become a current research hotspot in the field of sensors with very good application prospects. [25][26][27][28][29][30][31] The microcantilever detection technology is based on the optical lever method to read out the displacement of the laser on the position-sensitive detector, which has 10 3 orders of magnitude amplification as compared to the real deflection of the microcantilever tip. In addition, due to its relatively soft nature, the detection sensitivity will be further improved.…”

Section: Introductionmentioning

confidence: 99%

Magneto‐Nanomechanical Array Biosensor for Ultrasensitive Detection of Oncogenic Exosomes for Early Diagnosis of Cancers

Mei

Yan

Wang

et al. 2022

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The Advisory Committee on Cancer for the World Health Organization suggests that "a third of cancers are curable if diagnosed early". [3] Traditional methods of cancer diagnosis are based primarily on histo-pathological examination and imaging of the tumor tissue, which are the current gold standards. [4] However, these methods tend to detect specific lesions only when they are formed in the middle and later stages of cancer development, and are not suitable for screening early cancers in a large population. In the early stage of cancer development, before the formation of specific lesions, cancer cells in the body begin to release a few cancer markers related to cancer formation and metastasis. [5] At this time, if a highly sensitive detection method can be available to detect the very low concentration of markers in body fluids, it will bring great potential for the early detection and diagnosis of cancer. In addition, traditional methods are invasive, and inaccurate biopsy positioning can cause sampling errors. X-ray screening and histo-pathological evaluation rely heavily on the individual's expertise, which causes discrepancies between individual and laboratory-reported results. [6] All these limitations indicate the need for highly sensitive and minimally invasive techniques for early diagnosis and treatment of cancers. In comparison, liquid biopsies can offer early discovery and a non-invasive approach.In recent years, cancer markers in body fluids, such as circulating tumor-derived proteins (carcinoembryonic antigen, prostate-specific antigen), circulating tumor cells, and circulating tumor DNA, [7] have been the most common biomarkers for cancer diagnosis and assessment of treatment efficacy. However, these biomarkers have several shortcomings in early-stage cancer diagnosis. Circulating tumor-derived protein markers are compromised by high false positive rates, which can lead to overdiagnosis and, in some cases, to unnecessary anticancer treatment. [8] The fraction of circulating tumor DNA in total circulating free DNA is usually very low and often < 0.01%; therefore, this can complicate early detection. [9] Circulating tumor cells are tumor cells that have detached from the primary tumor, with extremely low quantities. Exosomes are a class of nanoscale vesicles secreted by cells, which contain abundant information closely related to parental cells. The ultrasensitive detection of cancer-derived exosomes is highly significant for early noninvasive diagnosis of cancer.Here, an ultrasensitive nanomechanical sensor is reported, which uses a magnetic-driven microcantilever array to selectively detect oncogenic exosomes. A magnetic force, which can produce a far greater deflection of microcantilever than that produced by the intermolecular interaction force even with very low concentrations of target substances, is introduced. This method reduced the detection limit to less than 10 exosomes mL −1 . Direct detection of exosomes in the serum of patients with breast cancer and in healthy people showed a signifi...

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Aptamer-based microcantilever-array biosensor for ultra-sensitive and rapid detection of okadaic acid

Cited by 20 publications

References 39 publications

A Genosensor Based on the Modification of a Microcantilever: A Review

A Genosensor Based on the Modification of a Microcantilever: A Review

Advances in Optical Fiber Aptasensor for Biochemical Sensing Applications

Magneto‐Nanomechanical Array Biosensor for Ultrasensitive Detection of Oncogenic Exosomes for Early Diagnosis of Cancers

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