Aptamer-based photoelectrochemical assay for the determination of MCF-7

Luo, Junjun; Liang, Dong; Li, Xiaoqing; Deng, Lei; Wang, Zaoxia; Yang, Minghui

doi:10.1007/s00604-020-04239-1

Cited by 32 publications

(21 citation statements)

References 45 publications

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“…Biocompatible hexagonal carbon nitride tubes (HCNTs) can improve light harvesting and enhance charge carrier transfer. HCNTs were utilized as the photoactive materials to develop an aptamer-based PEC method for the determination of MCF-7 cells [265]. After the binding of cells onto HCNTs, the photocurrent intensity was suppressed due to the steric hindrance effect.…”

Section: Photoelectrochemical Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

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Section: Photoelectrochemical Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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“…In the next study, a H 2 O 2 sensor employing a trimetallic AuPtPd nanocomposite and RGO nanosheets deposited on GCE was applied for the electrocatalytic detection of H 2 O 2 reduction with LOD of 2 nM (a linear range from 0.005 μM to 6.5 mM) [ 136 ]. The biosensor was applied for the detection of H 2 O 2 released by two BC cell lines (MDA-MB-231 and T47D) [ 137 ] (Approach 1).…”

Section: Nanomaterials/nanoparticles-based Electrochemical Biosensmentioning

confidence: 99%

“…Luo et al applied hexagonal carbon nitride tubes as a photoactive material to determine the photocurrent in the presence of MCF-7 cells (Approach 1) [ 137 ]. The cells were detected down to 17 cells/mL (a linear range from 100 to 1 × 10 5 cells/mL).…”

Section: Nanomaterials/nanoparticles-based Electrochemical Biosensmentioning

confidence: 99%

“…Glutaraldehyde was utilized as a cross-linker for the covalent immobilization of anti-MUC1 aptamers for the affinity capture of cells via surface-expressed MUC1. A clinical applicability of the biosensor was proved by the detection of cells spiked into blood samples at three different concentrations with a recovery index of 96%–104% [ 137 ].…”

Section: Nanomaterials/nanoparticles-based Electrochemical Biosensmentioning

confidence: 99%

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Electrochemical Nanobiosensors for Detection of Breast Cancer Biomarkers

Gajdošová

Lorencová

Kasák

et al. 2020

Sensors

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This comprehensive review paper describes recent advances made in the field of electrochemical nanobiosensors for the detection of breast cancer (BC) biomarkers such as specific genes, microRNA, proteins, circulating tumor cells, BC cell lines, and exosomes or exosome-derived biomarkers. Besides the description of key functional characteristics of electrochemical nanobiosensors, the reader can find basic statistic information about BC incidence and mortality, breast pathology, and current clinically used BC biomarkers. The final part of the review is focused on challenges that need to be addressed in order to apply electrochemical nanobiosensors in a clinical practice.

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“…Therefore, it is necessary to develop alternative molecular biological methods with high sensitivity, high accuracy and low cost for breast cancer diagnosis. There are numerous methods to detect MCF-7, including electrochemistry [15], electrochemiluminescence [16], colorimetry [17] and photoelectrochemical methods [18]. Aptamers, which are single-stranded DNA or RNA analogues to antibodies [19], have been attracting a lot of interest due to their advantages over traditional recognition molecules.…”

Section: Introductionmentioning

confidence: 99%

Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells

et al. 2021

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Arch-cruciform DNA are self-assembled on AuNPs/VS2 scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H2O2 reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10 − 1 × 105 cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.

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Aptamer-based photoelectrochemical assay for the determination of MCF-7

Cited by 32 publications

References 45 publications

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Electrochemical Nanobiosensors for Detection of Breast Cancer Biomarkers

Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells

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