Aptamer-based SERS biosensor for whole cell analytical detection of E. coli O157:H7

Díaz‐Amaya, Susana; Lin, Li‐Kai; Deering, Amanda J.; Stanciu, Lia

doi:10.1016/j.aca.2019.07.028

Cited by 113 publications

(26 citation statements)

References 30 publications

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“…80 Díaz-Amaya et al prepared aptamer and Raman reporter functionalised gold NP SERS tags for the highly specific and sensitive detection of E. coli O157:H7 using a SERS assay that utilised the phenomenon of cell sedimentation of SERS tagbacteria complexes within a food matrix and subsequent analysis of the residual supernatant. 81 This assay format combined with the specific aptameric SERS tag allowed for the detection and quantification of target bacteria within 20 minutes in pure culture and highly complex ground beef samples down to concentrations of 10 CFU mL −1 and 100 CFU mL −1 . The specificity of this platform was tested by comparing the target pathogen against S. aureus, E. coli B1201 and E. coli O55:H7 and observing that SERS signals obtained in the presence of interferent pathogens did not differ from controls performed without bacteria.…”

Section: Nucleic Acidsmentioning

confidence: 99%

Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications

Berry

Kearns

Graham

et al. 2021

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Section: Nucleic Acidsmentioning

confidence: 99%

Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications

Berry

Kearns

Graham

et al. 2021

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“…Recently, Stanciu and colleagues developed a surfaceenhanced Raman spectroscopy (SERS) based aptasensor for the detection of E. coli O157:H7 capable of detecting and quantifying ∼10 1 CFU/mL in pure culture and 10 2 CFU/mL in ground beef samples (Díaz-Amaya et al, 2019). In their design (Figure 2A), thiolated aptamers specific for E. coli O157:H7 were conjugated to the 4-aminothiophenol-AuNPs exploiting goldthiol interaction and the resulting aptamer/Raman reporters were used to selectively bind and coat E. coli O157:H7.…”

Section: Escherichia Colimentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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Due to their relative synthetic and chemical simplicity compared to antibodies, aptamers afford enhanced stability and functionality for the detection of environmental contaminants and for use in environmental monitoring. Furthermore, nucleic acid aptamers can be selected for toxic targets which may prove difficult for antibody development. Of particular relevance, aptamers have been selected and used to develop biosensors for environmental contaminants such as heavy metals, small-molecule agricultural toxins, and water-borne bacterial pathogens. This review will focus on recent aptamer-based developments for the detection of diverse environmental contaminants. Within this domain, aptamers have been combined with other technologies to develop biosensors with various signal outputs. The goal of much of this work is to develop cost-effective, user-friendly detection methods that can complement or replace traditional environmental monitoring strategies. This review will highlight recent examples in this area. Additionally, with innovative developments such as wearable devices, sentinel materials, and lab-on-a-chip designs, there exists significant potential for the development of multifunctional aptamer-based biosensors for environmental monitoring. Examples of these technologies will also be highlighted. Finally, a critical perspective on the field, and thoughts on future research directions will be offered.

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“…Zhu et al, 2015), aflatoxin B 1 in moldy sugarcane (Tian et al, 2018), and avian influenza virus particle in duck' feces (Kim et al, 2019) were reported. In another study, a highly sensitive detection of E. coli O157:H7 was achieved using SERS-aptamer based platform with the LOD of 10 CFU/mL in cell culture and 100 CFU/mL in ground beef samples (Díaz-Amaya, Lin, Deering, & Stanciu, 2019). The Raman probe-aptamers were covalently bound via 4-aminothiophenol to Au NPs.…”

Section: Other Plasmonic Sensorsmentioning

confidence: 99%

Plasmonic biosensors for food control

Balbinot

Srivastav

Vidić

et al. 2021

Trends in Food Science & Technology

121

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Background: Food safety is becoming increasingly important because food industry must provide quality products to minimize the health risks. Traditional methods to assure food safety, such as plate count and polymerase chain reaction are accurate and robust but can hardly satisfy the needs of the food industry because they are costly and time consuming. Therefore, optical biosensors that can analyze food in a low-cost, facile, fast, sensitive, and selective manner started to emerge. Scope and approach: This review presents plasmonic biosensors including surface plasmon resonance (SPR), localized SPR (LSPR), fiber optic SPR (FO-SPR), surface enhanced Raman scattering (SERS), surface-enhanced fluorescence (SEF), and total internal reflection (TIR) based sensors and their applications in food pathogens monitoring. Moreover, the strengths and weaknesses of plasmonic biosensors implementation in food control are showcased. Key findings and conclusions: Plasmonic biosensors could simplify procedure and radically reduce time, price and consummation of reactants, compared to traditional microbiological methods. Optical biosensors, in particular SPR, have been developed for detection of different foodborne pathogens. In parallel, analytical improvements have been achieved by coupling different techniques (fiber optics, Raman, fluorescence, luminescence) to plasmonic sensors in order to reduce the limits of detection and to improve sensitivity. The future improvements include the miniaturization of instruments to handheld devices and simplification of analysis to enable direct target detection in food matrices. Plasmonic technology can certainly have long lasting impact because the need for a simple and rapid food assay is pressing and guarantees the future development in this field.

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Aptamer-based SERS biosensor for whole cell analytical detection of E. coli O157:H7

Cited by 113 publications

References 30 publications

Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications

Surface enhanced Raman scattering for the multiplexed detection of pathogenic microorganisms: towards point-of-use applications

Aptamer-Based Biosensors for Environmental Monitoring

Plasmonic biosensors for food control

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