Aptamer based surface plasma resonance assay for direct detection of neuron specific enolase and progastrin-releasing peptide (31-98)

Sun, Linlin; Shen, Kemin; Wan, Wenjuan; Cao, Wenjun; Wang, Zhijun; Guo, Chaozhong

doi:10.1039/d1ra05041a

Cited by 13 publications

(6 citation statements)

References 40 publications

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“…The change in the SPR signal was used to quantify the ProGRP in the sample. The LOD of the proposed system was calculated to be 15.6 nM [ 105 ]. Wang et al reported a molecularly imprinted photoelectrochemical sensor for the sensitive detection of ProGRP lung cancer biomarkers.…”

Section: Biomarkers and Their Biosensorsmentioning

confidence: 99%

Emerging Biosensing Methods to Monitor Lung Cancer Biomarkers in Biological Samples: A Comprehensive Review

Chinnappan,

Mir,

Alsalameh

et al. 2023

Cancers

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Lung cancer is the most commonly diagnosed of all cancers and one of the leading causes of cancer deaths among men and women worldwide, causing 1.5 million deaths every year. Despite developments in cancer treatment technologies and new pharmaceutical products, high mortality and morbidity remain major challenges for researchers. More than 75% of lung cancer patients are diagnosed in advanced stages, leading to poor prognosis. Lung cancer is a multistep process associated with genetic and epigenetic abnormalities. Rapid, accurate, precise, and reliable detection of lung cancer biomarkers in biological fluids is essential for risk assessment for a given individual and mortality reduction. Traditional diagnostic tools are not sensitive enough to detect and diagnose lung cancer in the early stages. Therefore, the development of novel bioanalytical methods for early-stage screening and diagnosis is extremely important. Recently, biosensors have gained tremendous attention as an alternative to conventional methods because of their robustness, high sensitivity, inexpensiveness, and easy handling and deployment in point-of-care testing. This review provides an overview of the conventional methods currently used for lung cancer screening, classification, diagnosis, and prognosis, providing updates on research and developments in biosensor technology for the detection of lung cancer biomarkers in biological samples. Finally, it comments on recent advances and potential future challenges in the field of biosensors in the context of lung cancer diagnosis and point-of-care applications.

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Section: Biomarkers and Their Biosensorsmentioning

confidence: 99%

Emerging Biosensing Methods to Monitor Lung Cancer Biomarkers in Biological Samples: A Comprehensive Review

Chinnappan,

Mir,

Alsalameh

et al. 2023

Cancers

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“…21 Therefore, the NSE is one of the important biomarkers to be monitored. 22 Detection of NSE has been reported using various biosensing schemes, including surface plasmon resonance (SPR) spectroscopy, 23 surface plasmon-enhanced uorescence (SPF), 24,25 uorescence, 26 electrochemistry, [27][28][29] and photoelectrochemistry. 30,31 Nevertheless, most of the sensing methods rely on the development of nanomaterials that enhance or produce the sensor signals to detect NSE at its clinical threshold value.…”

Section: Introductionmentioning

confidence: 99%

Direct detection of neuron-specific enolase using a spectrometer-free colorimetric plasmonic biosensor

2023

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“…The concentration of NSE in serum rises significantly in patients with small cell lung cancer, neuroblastoma, or chemotherapy. As a tumor marker with high specificity, the rapid and reliable detection of NSE is critical for illness diagnosis and timely detection of diagnosis and treatment outcomes. , In this study, homogeneous and dense TiO 2 nanorod films were produced in situ on the surface of FTO and TiO 2 was sensitized by CdS. The TiO 2 /CdS composite materials can help to separate electrons and holes and increase visible light utilization.…”

Section: Introductionmentioning

confidence: 99%

Addressable Label-Free Photoelectric Sensor Array with Self-Calibration for Detection of Neuron Specific Enolase

et al. 2022

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An addressable label-free photoelectric immunosensor array was designed for detection of neuron specific enolase (NSE) based on TiO 2 /CdS as substrate materials. In this work, the hydrothermal synthesized TiO 2 nanorod film is evenly grown on the surface of the fluorine-doped tin oxide (FTO), and then CdS with a narrow band gap is added for sensitization through successive ionic layer adsorption reactions. The obtained TiO 2 /CdS composite materials with matched energy band structures promote the rapid electron transfer and effectively reduce the recombination of electron hole pairs, which greatly enhance the visible light absorption and increased photocurrent intensity. In order to construct a suitable sensor array, the sensitized FTO electrode is divided into multiple regions of equal size by insulating stickers, and then the addressable and continuous detection of multiple samples can be achieved. Because multiple detection regions are prepared and tested under the same conditions, the difference effectively reduces, and the sensor can realize self-calibration and obtain more accurate results. Under optimal conditions, this sensor array can detect NSE in the linear range of 0.01−100 ng mL −1 with a detection limit of 2.49 pg mL −1 (S/N = 3). The sensor array has good selectivity, stability, and reproducibility, making it a viable approach for real sample detection.

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Aptamer based surface plasma resonance assay for direct detection of neuron specific enolase and progastrin-releasing peptide (31-98)

Abstract: Direct detection of neuron specific enolase and progastrin-releasing peptide (31-98) were achieved by measuring the target-induced SPR response of an aptamer-coated chip.

Cited by 13 publications

References 40 publications

Emerging Biosensing Methods to Monitor Lung Cancer Biomarkers in Biological Samples: A Comprehensive Review

Emerging Biosensing Methods to Monitor Lung Cancer Biomarkers in Biological Samples: A Comprehensive Review

Direct detection of neuron-specific enolase using a spectrometer-free colorimetric plasmonic biosensor

Addressable Label-Free Photoelectric Sensor Array with Self-Calibration for Detection of Neuron Specific Enolase

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