Aptamer-Based Technology for Food Analysis

Abstract

Aptamers are short and functional single-stranded oligonucleotide sequences selected from systematic evolution of ligands by exponential enrichment (SELEX) process, which have the capacity to recognize various classes of target molecules with high affinity and specificity. Various analytical aptamers acquired by SELEX are widely used in many research fields, such as medicine, biology, and chemistry. However, the application of this innovative and emerging technology to food safety is just in infant stage. Food safety plays a very important role in our daily lives because varieties of poisonous and harmful substances in food affect human health. Aptamer technique is promising, which can overcome many disadvantages of existing detection methods in food safety, such as long detection time, low sensitivity, difficult, and expensive antibody preparation. This review provides an overview of various aptamer screening technologies and summarizes the recent applications of aptamers in food safety, and future prospects are also discussed.

Aptamer based surface plasmon resonance sensor for aflatoxin B1

“…Aptamers have been used in biosensors and versatile assays for a variety of targets [8][9][10][11]. The aptamer for AFB1 has been selected and provides new choices for detection of AFB1 using aptamer as affinity ligand [12].…”

Analysis of ATP and AMP binding to a DNA aptamer and its imidazole-tethered derivatives by surface plasmon resonance

“…1,2 They are obtained by a procedure referred to as systematic evolution of ligands by exponential enrichment (SELEX), [3][4][5] and have been used in various fields, including clinical [6][7][8][9] and food safety [10][11][12][13] applications. 1,2 They are obtained by a procedure referred to as systematic evolution of ligands by exponential enrichment (SELEX), [3][4][5] and have been used in various fields, including clinical [6][7][8][9] and food safety [10][11][12][13] applications.…”

Recent Advances in the Determination of Pesticides in Environmental Samples by Capillary Electrophoresis

“…Although remarkable achievements have been made in maximizing the detection sensitivity, substantial developmental challenges and opportunities still exist such as green and fast sample pretreatment with automation, high throughput and fast screening, and portable devices. For example, such a method would utilize a green sample preparation approach applicable to more complex matricies, and a portable equipment (microchip-CE or bio/nanosensors) [ 71 , 72 , 77 , 78 , 102 , 103 , 104 ] for the analysis, providing a real-time response of the pesticide residues. Due to the excellent selectivity and specificity of MS detection and the information provided about the molecular weights and structures of the solutes, more applications of CE-MS to the quantitative analysis of pesticides and their metabolites.…”