Aptamer-based trapping of phytosphingosine in urine samples

Fischer, Christin; Klockmann, Sven; Wessels, Hauke; Hünniger, Tim; Schrader, Jil; Paschke-Kratzin, Angelika; Fischer, Markus

doi:10.1016/j.jbiotec.2016.09.005

Cited by 7 publications

(9 citation statements)

References 25 publications

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“…In general, gained dissociation constants are slightly comparable to those of published aptamers toward small molecule targets . Moreover, it is worth mentioning that the dissociation constants are comparable to those published for antibodies. − The used just in time selection seems to be a successful tool for aptamer selection toward (i) proteins, (ii) metabolites, and, as shown in this study, (iii) antibiotics. , Due to the half-automatization of just in time selection, this process features high reproducibility, lower costs, and less hands-on time.…”

Section: Resultssupporting

confidence: 77%

“…28−30 The used just in time selection seems to be a successful tool for aptamer selection toward (i) proteins, (ii) metabolites, and, as shown in this study, (iii) antibiotics. 15,18 Due to the half-automatization of just in time selection, this process features high reproducibility, lower costs, and less hands-on time.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…So far, only manual aptamer-based enrichment approaches have been published. 14,15 Thereby, automation of trapping enables a higher reproducibility by less hands-on time. Automated applications are up-to-date, more cost-effective, and independent of executing staff.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Due to the missing complementary strand, intramolecular interactions enable the formation of sequence-specific three-dimensional structures, which induce the high affinity of aptamers toward their target. − Thus, a large range of potential aptamer-based applications is enabled. − The aptamer-based trapping, as one of these applications, is usable as it is immunologically pendant, for, for example, cleanup or enrichment strategies. So far, only manual aptamer-based enrichment approaches have been published. , Thereby, automation of trapping enables a higher reproducibility by less hands-on time. Automated applications are up-to-date, more cost-effective, and independent of executing staff.…”

Section: Introductionmentioning

confidence: 99%

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Automated Enrichment of Sulfanilamide in Milk Matrices by Utilization of Aptamer-Linked Magnetic Particles

Fischer

Kallinich

Klockmann

et al. 2016

J. Agric. Food Chem.

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The present work demonstrates the first automated enrichment approach for antibiotics in milk using specific DNA aptamers. First, aptamers toward the antibiotic sulfanilamide were selected and characterized regarding their dissociation constants and specificity toward relevant antibiotics via fluorescence assay and LC-MS/MS detection. The performed enrichment was automated using the KingFisherDuo and compared to a manual approach. Verifying the functionality, trapping was realized in different milk matrices: (i) 0.3% fat milk, (ii) 1.5% fat milk, (iii) 3.5% fat milk, and (iv) 0.3% fat cocoa milk drink. Enrichment factors up to 8-fold could be achieved. Furthermore, it could be shown that novel implementation of a magnetic separator increases the reproducibility and reduces the hands-on time from approximately half a day to 30 min.

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Section: Resultssupporting

confidence: 77%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Automated Enrichment of Sulfanilamide in Milk Matrices by Utilization of Aptamer-Linked Magnetic Particles

Fischer

Kallinich

Klockmann

et al. 2016

J. Agric. Food Chem.

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“…A feasible approach for detecting rare analytes is immuno-capture or aptamer trapping, wherein immobilized antibodies or aptamers are used for an initial enrichment step 42 , 43 . To detect proteins and other small molecules, aptamer trapping can be used for target capture before LC-MS or other analyses 42 , 44 . Aptamer-mediated pathogen capture is frequently combined with PCR or other nucleic acid amplification technologies to increase detection sensitivity.…”

Section: Potential Utility Of Aptamer Technology For Pathogen Detectionmentioning

confidence: 99%

Oligonucleotide aptamers for pathogen detection and infectious disease control

Wan

Liu

2021

Theranostics

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During an epidemic or pandemic, the primary task is to rapidly develop precise diagnostic approaches and effective therapeutics. Oligonucleotide aptamer-based pathogen detection assays and control therapeutics are promising, as aptamers that specifically recognize and block pathogens can be quickly developed and produced through simple chemical synthesis. This work reviews common aptamer-based diagnostic techniques for communicable diseases and summarizes currently available aptamers that target various pathogens, including the SARS-CoV-2 virus. Moreover, this review discusses how oligonucleotide aptamers might be leveraged to control pathogen propagation and improve host immune system responses. This review offers a comprehensive data source to the further develop aptamer-based diagnostics and therapeutics specific for infectious diseases.

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