Aptamer biosensor for protein detection based on guanine-quenching

Wang, Wen‐Juan; Chen, Chunlai; Qian, Min; Zhao, Xin

doi:10.1016/j.snb.2007.07.125

Cited by 47 publications

(42 citation statements)

References 43 publications

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“…Recently our group has developed a protein sensor based on G-quenching of TMR in an aptamer, and it was found that the quenching efficiency is sensitive to the position of guanosine in the DNA strand [31] . Fluorescence correlation spectroscopy (FCS) has shown the power and feasibility in monitoring fast kinetic processes with a time resolution at sub-microsecond scale [5,30,[32][33][34][35][36] .…”

Section: Introductionmentioning

confidence: 99%

Fluorescence quenching of TMR by guanosine in oligonucleotides

Chen

Zhou

et al. 2009

Sci. China Ser. B-Chem.

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Nucleotide-specific fluorescence quenching in fluorescently labeled DNA has many applications in biotechnology. We have studied the inter-and intra-molecular quenching of tetramethylrhodamine (TMR) by nucleotides to better understand their quenching mechanism and influencing factors. In agreement with previous work, dGMP can effectively quench TMR, while the quenching of TMR by other nucleotides is negligible. The Stern-Volmer plot between TMR and dGMP delivers a bimolecular quenching constant of K s = 52.3 M −1 . The fluorescence of TMR in labeled oligonucleotides decreases efficiently through photoinduced electron transfer by guanosine. The quenching rate constant between TMR and guanosine was measured using fluorescence correlation spectroscopy (FCS). In addition, our data show that the steric hindrance by bases around guanosine has significant effect on the G-quenching. The availability of these data should be useful in designing fluorescent oligonucleotides and understanding the G-quenching process.

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Section: Introductionmentioning

confidence: 99%

Fluorescence quenching of TMR by guanosine in oligonucleotides

Chen

Zhou

et al. 2009

Sci. China Ser. B-Chem.

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“…This result may be explained by the bindinginduced closeness of FAM to G bases on the other end of Th27-3-FAM, and G could cause uorescence quenching. 13 In contrast, in Th27-5-FAM, FAM was labeled at the 5'end of the aptamer, already close to a G base, so the uorescence intensity of FAM was lower and the binding-induced uorescence change was smaller. Instead of the 27-mer aptamer, the 29-mer aptamer with FAM labeled at the 3 0 end (Th29-3-FAM) was also tested.…”

Section: Resultsmentioning

confidence: 97%

“…The binding of thrombin to the aptamer causes the structural change of the aptamer, 14 and the uorescence intensity of the labeled FAM is quenched due to possible changes in the local environment of FAM (e.g. proximity to bases of the aptamer or the bound thrombin) or its possible interaction with bases like G of the aptamer 13 or thrombin. We rst tested the feasibility of the assay for thrombin by using the 27-mer aptamer with FAM labeled at the 3 0 end (Th27-3-FAM).…”

Section: Resultsmentioning

confidence: 99%

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A fluorescein labeled aptamer switch for thrombin with fluorescence decrease response

Líu

Zhao

2015

Anal. Methods

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We developed a simple fluorescent switch for rapid thrombin detection by using a high-binding affinity 27-mer aptamer with fluorescein (FAM) labeled at the 3 0 end. This FAM-labeled aptamer showed remarkable fluorescence quenching upon thrombin binding. This assay enabled the detection of thrombin ranging from 0.062 nM to 2 nM. The assay was selective, and other tested proteins and diluted serum did not interfere with thrombin analysis.

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“…Despite the wide literature on the use of aptamers in biosensors, it seems that most of publications are reviews [197] while the experimental studies are still quite few. These latter studies can be grouped on the basis of aptamers associated to quantum dots (QDs) [142,198], nanoparticles [195,199,200], carbon nanotubes [201], or label-free [202] as compared with antibodiesbased studies [203]. Lee et al (2012) [204] were the only ones, to the best of our knowledge, who described the use of an aptamer-immobilised electrospun polystyrene-poly(styrene-co-maleicanhydride) (PS-PSMA) nanofibre as a new cost-effective aptasensor platform for protein detection (Fig.…”

Section: Aptamers Biosensorsmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

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Aptamer biosensor for protein detection based on guanine-quenching

Cited by 47 publications

References 43 publications

Fluorescence quenching of TMR by guanosine in oligonucleotides

Fluorescence quenching of TMR by guanosine in oligonucleotides

A fluorescein labeled aptamer switch for thrombin with fluorescence decrease response

Electrospinning-Based Nanobiosensors

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