Aptamer cell sensor based on porous graphene oxide decorated ion-selective-electrode: Double sensing platform for cell and ion

Porous Graphene Oxide Decorated Ion Selective Electrode for Observing Across-Cytomembrane Ion Transport

“…By comparing the fitted lines of PGO-ISEs before (dashed) and after (solid) cells’ immobilization, it could be found, though the I − sensitivity is still possessed by the cells anchored electrodes, that the slopes (K) of the solid lines are lower than those of the dashed lines. This phenomenon agrees with the experimental result in our previous work [ 24 ]. What’s more, in this work, two NIS related issues are pointed out by comparing the changed data (ΔV out ) and fitted lines of TG (orange) and CG (blue) fixed PGO-ISEs.…”

“…However, there are still exceptions as indicated by the dotted boxes in Figure 5 D, for the reason of the small deviation, we speculate that it may have been the interference of Cl − , which happens in all kinds of cells [ 27 ], which may have lead to the pseudo-responding of I − -ISE, as evidenced by our previous work [ 24 ]. In addition, the illustrations for the non-zero deviation ratios induced by the fixed CG cells (the gray columns) and NIU cells are also speculated here.…”

“…The schematic diagram of ISE is depicted in Figure 1 A, the detailed description about the principle of ISE can be found in our previous work [ 24 ]. According to the Nernst equation, ISE’s V out is in a linear relationship with the logarithm of the I − ions’ concentration (C I ) at the interface between the Ag 2 S/AgI crystal film and the solution (as diagramed in Figure 1 A).…”

“…As illustrated in Figure 1 A, a porous graphene oxide (PGO) scaffold IME is built on the iodide sensitive ISE (I − -ISE) with the sensing film of Ag 2 S/AgI. In our previous work, it was proven that the PGO decoration on ISE could provide a robust three-dimensional (3D) and biocompatible media for the immobilized cells [ 24 ]. We conjecture (as depicted in Figure 1 B) that the PGO decorated I − -ISE (PGO-ISE) could be developed as an alternative device for observing the ionic fluctuation induced by the across-cytomembrane ion transport, i.e., the different I − distributions between the cells in the tested group (TG) and the control group (CG), which are with and without NIS expression on their cytomembrane, respectively.…”

ASPECT, an LDA-Based Predictive Algorithm for In Vitro Selection

“…DNA and RNA aptamers are sequences that bind to specific target molecules. Aptamers have been found for many targets, ranging from small molecules such as kanamycin [12], to large protein complexes such as human thrombin [13], and even entire cells exemplified as human lung carcinoma cell A549 [14].…”