Aptamer-Conjugated Micelles for Targeted Photodynamic Therapy Via Photoinitiated Polymerization-Induced Self-Assembly

Abstract

Here, we report photoinitiated polymerization-induced self-assembly (photo-PISA) mediated by Rose Bengal methacrylate (RBMA) to reproducibly construct nanoparticles in one pot, on a large scale, for photodynamic therapy (PDT). In this work, we take advantage of the multifunctionality of RBMA by applying the monomer as a photocatalyst for self-catalyzed PISA, a singlet oxygen (1O2) generator for PDT, and a fluorophore for imaging. Covalent incorporation of Rose Bengal (RB) into nanostructures by the polymerizable methacrylate moiety permitted quantitative and controlled loading. When activated by visible light in aqueous solution, the RB within the cores of micelles yielded 1O2 more efficiently than free RB. Post-PISA modification of the RB-loaded nanocarriers with a DNA aptamer enhanced internalization by HCT116 cells and significantly inhibited tumor cell proliferation when exposed to yellow light. Such aptamer-labeled nanoparticles, prepared for PDT, extend the practical biomedical applications of PISA and are potential candidates for clinical cancer treatment.

RAFT-mediated polymerization-induced self-assembly (RAFT-PISA): current status and future directions

“…In 2021, Sumerlin et al reported a strategy to maximize the multifunctionality of Rose Bengal Methacrylate (RBMA) as both photocatalyst and comonomer in a photo-PISA process, also a singlet oxygen generator for PDT, and a fluorophore for imaging. 158 In their work, quantitative and controlled loading of Rose Bengal (RB) was realized by converting RB to the polymerizable methacrylate that could be covalently incorporated into nanoparticles. It was further demonstrated that RB-loaded nanoparticles can be activated by visible light, and the efficiency of singlet oxygen production was higher than free RB.…”

Importance of Rose Bengal Loaded with Nanoparticles for Anti-Cancer Photodynamic Therapy

“…Korpusik et al [78] reported the design of a targeted micelle-based PDT system (Sgc8 micelles, hydrodynamic diameter of 52 nm) based on azide-functionalized polymeric micelles decorated with protein tyrosine kinase 7 (PTK7)-binding DNA (Deoxyribonucleic acid) aptamer (Sgc8) via click chemistry. The covalent conjugation of RB to micelles provided quantitative and controlled RB loading.…”

Photocatalytic polymer nanomaterials for the production of high value compounds

“…The enhanced distribution lead to increased singlet oxygen production, creating a promising material for use in photodynamic therapy. 54…”