Aptamer-Conjugated Quantum Dot Optical Biosensors: Strategies and Applications

Kim, Dong-Min; Yoo, Seung-Min

doi:10.3390/chemosensors9110318

Cited by 16 publications

(7 citation statements)

References 111 publications

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“…Quantum dots (QDs) are ideal optical labels for highly sensitive multiple detection in in vitro diagnostics (IVD) due to their unique optical properties. , Optical biosensors based on QDs have been widely used to detect a wide range of biomarkers, small molecule toxins, etc . − The accuracy and sensitivity of obtaining more biological information from low volume will be the future trend in the development of quantitative detections in IVD, , especially for highly sensitive and disease-specific biomarker detection, such as those associated with infectious diseases . A delayed early diagnosis and timely treatment of infectious diseases can result in sepsis, multiple organ dysfunction syndrome (MODS), and even life-threatening disease progression. − Procalcitonin (PCT) and Interleukin-6 (IL-6), among the many highly sensitive and specific inflammatory factors, are new effective indexes for assessing the severity of bacterial infection, determining the prognosis of the disease, evaluating the effectiveness of antibiotic use, and guiding clinical treatment .…”

Section: Introductionmentioning

confidence: 99%

Development of a Dual Fluorescence Signal-Enhancement Immunosensor Based on Substrate Modification for Simultaneous Detection of Interleukin-6 and Procalcitonin

Zhao,

Yang,

et al. 2024

Langmuir

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High-sensitivity detection of biomarkers is of great significance to improve the accuracy of disease diagnosis and the rate of occult disease diagnosis. Using a substrate modification and two-color quantum dot (QD) nanobeads (QBs), we have developed a dual fluorescence signal-enhancement immunosensor for sensitive, simultaneous detection of interleukin 6 (IL-6) and procalcitonin (PCT) at low volumes (∼20 μL). First, the QBs compatible with QDs with different surface ligands were prepared by optimizing surfactants based on the microemulsion method. Through the use of a fluorescence-linked immunosorbent assay (FLISA), the feasibility of a dual signal-enhancement immunosensor was verified, and a 5-fold enhancement of fluorescence intensity was achieved after the directional coating of the antibodies on sulfhydryl functionalization (−SH) substrates and the preparation of QBs by using a polymer and silica double-protection method. Next, a simple polydimethylsiloxane (HS-PDMS) immunosensor with a low volume consumption was prepared. Under optimal conditions, we achieved the simultaneous detection of IL-6 and PCT with a linear range of 0.05−50 ng/mL, and the limit of detection (LOD) was 24 and 32 pg/ mL, respectively. The result is comparable to two-color QBs-FLISA with a sulfhydryl microplate, even though only 20% of its volume was used. Thus, the dual fluorescence signal-enhancement HS-PDMS immunosensor offers the capability of early microvolume diagnosis of diseases, while the detection of inflammatory factors is clinically important for assisting disease diagnosis and determining disease progression.

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Section: Introductionmentioning

confidence: 99%

Development of a Dual Fluorescence Signal-Enhancement Immunosensor Based on Substrate Modification for Simultaneous Detection of Interleukin-6 and Procalcitonin

Zhao,

Yang,

et al. 2024

Langmuir

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“…In their recent publication, Kim et al illustrate various examples of sensing methodologies that leverage optical signal enhancement and quenching of QDs. Moreover, the authors propose potential strategies to expand the practical applications of biosensors across a range of scientific and technological domains 30 . Considering the findings of the studies conducted on using QDs and Apts in bioassays, safe QD‐Apts must be developed for accurate detection and combination with drugs to develop an effective theranostic agent.…”

Section: Introductionmentioning

confidence: 99%

Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends

Davodabadi

Mirinejad

Fathi-karkan

et al. 2023

Biotechnology Progress

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Aptamers (Apts) are synthetic nucleic acid ligands that can be engineered to target various molecules, including amino acids, proteins, and pharmaceuticals. Through a series of adsorption, recovery, and amplification steps, Apts are extracted from combinatorial libraries of synthesized nucleic acids. Using aptasensors in bioanalysis and biomedicine can be improved by combining them with nanomaterials. Moreover, Apt‐associated nanomaterials, including liposomes, polymeric, dendrimers, carbon nanomaterials, silica, nanorods, magnetic NPs, and quantum dots (QDs), have been widely used as promising nanotools in biomedicine. Following surface modifications and conjugation with appropriate functional groups, these nanomaterials can be successfully used in aptasensing. Advanced biological assays can use Apts immobilized on QD surfaces through physical interaction and chemical bonding. Accordingly, modern QD aptasensing platforms rely on interactions between QDs, Apts, and targets to detect them. QD‐Apt conjugates can be used to directly detect prostate, ovarian, colorectal, and lung cancers or simultaneously detect biomarkers associated with these malignancies. Tenascin‐C, mucin 1, prostate‐specific antigen, prostate‐specific membrane antigen, nucleolin, growth factors, and exosomes are among the cancer biomarkers that can be sensitively detected using such bioconjugates. Furthermore, Apt‐conjugated QDs have shown great potential for controlling bacterial infections such as Bacillus thuringiensis, Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, Campylobacter jejuni, Staphylococcus aureus, and Salmonella typhimurium. This comprehensive review discusses recent advancements in the design of QD‐Apt bioconjugates and their applications in cancer and bacterial theranostics.

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“…The presence of analytes such as albumin induces the release of an aptamer to a target, resulting in fluorescence recovery. Several studies indicate the high sensitivity of GQD-based aptasensors, ,, but the molecular insight into the formation of GQD-aptamer-GHSA complexes in GQD-based aptasensors is still limited. Especially, how albumin recognizes and interacts with a GQD-bound aptamer in an aqueous solution remains unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Two different albumin orientations (front and back poses facing GQDA) are investigated to mimic possible binding poses of albumin onto the aptamer-GQD complex (Figure C). Both front and back orientations used here are based on previously reported ligand-binding sites on an albumin surface. − The insight obtained here will serve as a base for accurate GQD-based aptasensor design and development.…”

Section: Introductionmentioning

confidence: 99%

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

et al. 2023

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Diabetes mellitus is a chronic metabolic disease involving continued elevated blood glucose levels. It is a leading cause of mortality and reduced life expectancy. Glycated human serum albumin (GHSA) has been reported to be a potential diabetes biomarker. A nanomaterial-based aptasensor is one of the effective techniques to detect GHSA. Graphene quantum dots (GQDs) have been widely used in aptasensors as an aptamer fluorescence quencher due to their high biocompatibility and sensitivity. GHSA-selective fluorescent aptamers are first quenched upon binding to GQDs. The presence of albumin targets results in the release of aptamers to albumin and consequently fluorescence recovery. To date, the molecular details on how GQDs interact with GHSA-selective aptamers and albumin remain limited, especially the interactions of an aptamer-bound GQD (GQDA) with an albumin. Thus, in this work, molecular dynamics simulations were used to reveal the binding mechanism of human serum albumin (HSA) and GHSA to GQDA. The results show the rapid and spontaneous assembly of albumin and GQDA. Multiple sites of albumins can accommodate both aptamers and GQDs. This suggests that the saturation of aptamers on GQDs is required for accurate albumin detection. Guanine and thymine are keys for albumin-aptamer clustering. GHSA gets denatured more than HSA. The presence of bound GQDA on GHSA widens the entrance of drug site I, resulting in the release of open-chain glucose. The insight obtained here will serve as a base for accurate GQD-based aptasensor design and development.

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Aptamer-Conjugated Quantum Dot Optical Biosensors: Strategies and Applications

Cited by 16 publications

References 111 publications

Development of a Dual Fluorescence Signal-Enhancement Immunosensor Based on Substrate Modification for Simultaneous Detection of Interleukin-6 and Procalcitonin

Development of a Dual Fluorescence Signal-Enhancement Immunosensor Based on Substrate Modification for Simultaneous Detection of Interleukin-6 and Procalcitonin

Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

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