Aptamer‐Functionalized Barcode Particles for the Capture and Detection of Multiple Types of Circulating Tumor Cells

Zheng, Fuyin; Cheng, Yao; Wang, Jie; Lu, Jie; Zhang, Bin; Zhao, Yuanjin; Gu, Zhongze

doi:10.1002/adma.201403530

Cited by 171 publications

(140 citation statements)

References 38 publications

Supporting

Mentioning

139

Contrasting

Unclassified

Order By: Relevance

“…These issues, together with debatable biocompatibility, limit the applications of the barcode particles in multiple-cell research. [ 209,211 ] To solve these problems, biocompatible biomaterials and silica hybrid PCBs were suggested for cell culture ( Figure 18 ). [ 212 ] The size of the PCBs can be adjusted by microfl uidics to be suitable for cell culture.…”

Section: Cell Culturementioning

confidence: 99%

Microfluidic Synthesis of Barcode Particles for Multiplex Assays

Zhao

Cheng

Shang

et al. 2014

Small

Self Cite

192

139

View full text Add to dashboard Cite

The increasing use of high-throughput assays in biomedical applications, including drug discovery and clinical diagnostics, demands effective strategies for multiplexing. One promising strategy is the use of barcode particles that encode information about their specific compositions and enable simple identification. Various encoding mechanisms, including spectroscopic, graphical, electronic, and physical encoding, have been proposed for the provision of sufficient identification codes for the barcode particles. These particles are synthesized in various ways. Microfluidics is an effective approach that has created exciting avenues of scientific research in barcode particle synthesis. The resultant particles have found important application in the detection of multiple biological species as they have properties of high flexibility, fast reaction times, less reagent consumption, and good repeatability. In this paper, research progress in the microfluidic synthesis of barcode particles for multiplex assays is discussed. After introducing the general developing strategies of the barcode particles, the focus is on studies of microfluidics, including their design, fabrication, and application in the generation of barcode particles. Applications of the achieved barcode particles in multiplex assays will be described and emphasized. The prospects for future development of these barcode particles are also presented.

show abstract

Section: Cell Culturementioning

confidence: 99%

Microfluidic Synthesis of Barcode Particles for Multiplex Assays

Zhao

Cheng

Shang

et al. 2014

Small

Self Cite

192

139

View full text Add to dashboard Cite

show abstract

“…Zheng et al recently described a novel strategy whereby cancer cells spiked into blood can be captured and detected with DNA aptamer-functionalized spherical colloidal crystal clusters (Figure 4B) [77]. The reflective properties of the colloidal crystal structures, which can be controlled during their fabrication, allows for their use as “barcode particles.” These specifically varied reflective properties are utilized as a way of detecting and evaluating the identity of the particular particles (e.g.…”

Section: Aptamer-based Technologies For Ctc Detectionmentioning

confidence: 99%

Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells

Dickey

Giangrande

2016

Methods

View full text Add to dashboard Cite

A critical challenge for treating cancer is the early identification of those patients who are at greatest risk of developing metastatic disease. The number of circulating tumor cells (CTCs) in cancer patients has recently been shown to be a valuable (and non-invasively accessible) diagnostic indicator of the state of metastatic disease. CTCs are rare cancer cells found in the blood circulation of cancer patients believed to provide a means of diagnosing the likelihood for metastatic spread and assessing response to therapy in advanced, as well as early stage disease settings. Numerous technical efforts have been made to reliably detect and quantify CTCs, but the development of a universal assay has proven quite difficult. Notable challenges for developing a broadly useful CTC-based diagnostic assay are the development of easy-to-operate methods that 1) are sufficiently sensitive to reliably detect the small number of CTCs that are present in the circulation and 2) can capture the molecular heterogeneity of tumor cells. In this review, we describe recent progress towards the application of synthetic oligonucleotide aptamers as promising, novel, robust tools for the isolation and detection of CTCs. Advantages and challenges of the aptamer approach are also discussed.

show abstract

“…Der "heilige Gral" der CTC-Isolierung ist die Gewinnung der Zellen ohne spezifische Marker.W ährend neue Te chnologien auf vielfältige Marker zugreifen [53,57,58] und Multivalenz für ein hochspezifisches Einfangen von Zellen forcieren, [58][59][60] kçnnen nach markerfreier Isolierung CTCs mit noch uncharakterisiertem Phänotyp analysiert werden. Markerfreie Isolierung vermeidet einen Selektionsdruck, der vom Einfangen von Zellen basierend auf spezifischen Oberflächenmarkern herrührt.…”

Section: Markerfreie Isolierung Von Ctcsunclassified

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Green

Safaei²,

Mepham

et al. 2015

Angewandte Chemie

View full text Add to dashboard Cite

In den letzten zehn Jahren gab es wichtige Fortschritte bei der Erfassung seltener zirkulierender Tumorzellen (CTCs) aus dem Blut von Krebspatienten als eine entscheidende Voraussetzung für die nichtinvasive Tumorprofilierung. Diese Fortschritte haben zudem neue Einblicke in die Materialchemie und die Mikrofluidik erbracht und ermöglichten die Erfassung und Auszählung von CTCs mit beispielloser Empfindlichkeit. Allerdings wurde auch immer klarer, dass einfaches Isolieren und Auszählen von Tumorzellen, die in den Kreislauf gelangt waren, nicht die benötigten Informationen lieferte, um unser Verständnis der Biologie dieser seltenen Zellen zu vertiefen oder um sie sich in der Therapie besser zunutze zu machen. Mithilfe von Apparaturen und Materialien der nächsten Generation mit maßgeschneiderten physikalischen und chemischen Eigenschaften ist man inzwischen in der Lage, mehr Informationen aus CTCs zu gewinnen. In diesem Kurzaufsatz werden die Arbeiten der letzten zehn Jahre in diesem Gebiet diskutiert, wobei insbesondere die bahnbrechenden Studien der letzten fünf Jahre Berücksichtigung finden, die den Blick über das einfache Isolieren von CTCs hinaus auf Ansätze gerichtet haben, die eine tiefergehende Analyse ermöglichen.

show abstract

Aptamer‐Functionalized Barcode Particles for the Capture and Detection of Multiple Types of Circulating Tumor Cells

Cited by 171 publications

References 38 publications

Microfluidic Synthesis of Barcode Particles for Multiplex Assays

Microfluidic Synthesis of Barcode Particles for Multiplex Assays

Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Contact Info

Product

Resources

About