Aptamer Functionalized DNA Hydrogel for Wise-Stage Controlled Protein Release

Liu, Chen; Han, Jialun; Pei, Yuxuan; Du, Jie

doi:10.3390/app8101941

Cited by 23 publications

(21 citation statements)

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“…As previously discussed, DNA-hydrogels are biodegradable and, in principle, the versatile nature of DNA sequences and their ability to respond to external stimuli allow their utilization for efficient encapsulation and release of drugs and other molecules. Several systems based on DNA-hydrogels made of NSs have been designed for loading and release of drugs (Um et al, 2006;Nishikawa et al, 2011;Nishikawa et al, 2014;Song et al, 2015b;Ishii-Mizuno et al, 2017;Umeki et al, 2017;Wei et al, 2019;Yue et al, 2019), nucleic acids, (Li et al, 2015;Nishida et al, 2016;Ma et al, 2018;Song et al, 2018;Komura et al, 2020), and proteins (Liu et al, 2018). The DNA-hydrogel can easily trap molecules by varying the stiffness and pore size of the DNA structure, as shown by Yue et al (2019).…”

Section: Dna-hydrogels For Delivery Of Drugs and Nucleic Acidsmentioning

confidence: 99%

“…Currently, cell viability is the most analyzed parameter. It was found that DNA-gels did not cause alteration of viability in human dermal and tumor cell lines, and in mouse macrophage-like cells treated with trimeric or tetrameric NSs DNA-gels (Liu et al, 2018;Obuobi et al, 2019;Wei et al, 2019;Yue et al, 2019), and with NSs complexed with gold nanoparticles (Song et al, 2015a;Song et al, 2015b). The evaluation of cell death pathways is necessary, together with viability, since programmed cell death is a common outcome after nanoparticles exposure (Namvar et al, 2015;Sun et al, 2018;Fritsch-Decker et al, 2019).…”

Section: Biological Effects Of Dna-hydrogels On Cell Cultures and Promentioning

confidence: 99%

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DNA-GEL, Novel Nanomaterial for Biomedical Applications and Delivery of Bioactive Molecules

et al. 2020

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Novel DNA materials promise unpredictable perspectives for applications in cell biology. The realization of DNA-hydrogels built by a controlled association of DNA nanostars, whose binding can be tuned with minor changes in the nucleotide sequences, has been recently described. DNA hydrogels, with specific gelation properties that can be reassambled in desired culture media supplemented with drugs, RNA, DNA molecules and other bioactive compounds offer the opportunity to develop a novel nanomaterial for the delivery of single or multiple drugs in tumor tissues as an innovative and promising strategy. We provide here a comprehensive description of different, recently realized DNAgels with the perspective of stimulating their biomedical application. Finally, we discuss the possibility to design sophisticated 3D tissue-like DNA-gels incorporating cell spheroids or single cells for the assembly of a novel kind of cellular matrix as a preclinical investigation for the implementation of tools for in vivo delivery of bioactive molecules.

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Section: Dna-hydrogels For Delivery Of Drugs and Nucleic Acidsmentioning

confidence: 99%

Section: Biological Effects Of Dna-hydrogels On Cell Cultures and Promentioning

confidence: 99%

DNA-GEL, Novel Nanomaterial for Biomedical Applications and Delivery of Bioactive Molecules

et al. 2020

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“…DNA-functionalized gold nanoparticles or liposomes to DNA-functionalized hydrogels, when thrombin was added, a stable G-quadruplex structure emerged in the aptamer structure, which looked like a molecular switch between tight and relaxed states (95). X-shaped DNA, a DNA linker, and an aptamer were used to create a DNA hydrogel through the one-pot and the aptamer was only used as a functional unit for the target protein capture (96). A DNA nanohydrogel was developed to efficiently take up cells due to the recognition of an aptamer in the nanohydrogel (97).…”

Section: Aptamer As Bioactive Groups or As Tags For Functionalizationmentioning

confidence: 99%

Design, Bioanalytical, and Biomedical Applications of Aptamer-Based Hydrogels

Wang

et al. 2020

Front. Med.

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Aptamers are special types of single-stranded DNA generated by a process called systematic evolution of ligands by exponential enrichment (SELEX). Due to significant advances in the chemical synthesis and biotechnological production, aptamers have gained considerable attention as versatile building blocks for the next generation of soft materials. Hydrogels are high water-retainable materials with a three-dimensional (3D) polymeric network. Aptamers, as a vital element, have greatly expanded the applications of hydrogels. Due to their biocompatibility, selective binding, and molecular recognition, aptamer-based hydrogels can be utilized for bioanalytical and biomedical applications. In this review, we focus on the latest strategies of aptamer-based hydrogels in bioanalytical and biomedical applications. We begin this review with an overview of the underlying design principles for the construction of aptamer-based hydrogels. Next, we will discuss some bioanalytical and biomedical applications of aptamer-based hydrogel including biosensing, target capture and release, logic devices, gene and cancer therapy. Finally, the recent progress of aptamer-based hydrogels is discussed, along with challenges and future perspectives.

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“…DNA is a polymer material required for hydrogel formation. Du reported that aptamer-functionalized DNA hydrogels can serve as an appropriate candidate for controlled protein delivery by controlling complementary sequences as a biomolecular trigger [12].For soft tissue replacement, hydrogels are a suitable choice. Polyvinyl alcohol (PVA) has been extensively studied as an articular cartilage material [13,14].…”

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confidence: 99%

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Special Issue: Nanocomposite Hydrogels for Biomedical Applications

Matsumura¹

2020

Applied Sciences

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A hydrogel consists of a three-dimensional network of polymer chains, with water as a solvent in the system. Since the study by Wichterle and Lim on poly (2-hydroxyethyl methacrylate) hydrogels [1], these materials have been widely studied in the fields of tissue engineering, drug delivery systems (DDSs), artificial organs, and implantable materials [2] due to their high biocompatibility and high water content, which are similar to human body tissue. Biodegradable hydrogels are used for constructing cell scaffolds in tissue engineering, regenerative medicine, and DDS. Naturally derived, polymer-based hydrogels that are biodegradable and exhibit low toxicity can be used not only in tissue engineering but also as DDS carriers. Polysaccharide hydrogels are one of the most widely used polymeric systems and can be easily modified using their hydroxyl groups. In tissue engineering, alginate, dextran, chitosan, and polypeptide hydrogels have been studied to regenerate bone and skin tissues [3,4] and develop anticancer drugs, antimicrobial drugs, growth factors, and protein delivery systems [5,6]. Additionally, synthetic polymer-derived hydrogels have biomedical applications because it is easy to control the characters of hydrogels. For example, polyethylene glycol hydrogels have been used for cell culture scaffolds owing to the introduction of enzymatically degradable moieties [7].Nanocomposite polymer hydrogels are defined as cross-linked polymer networks swollen with water in the presence of nanoparticles and nanostructures. The goal of this special issue is to showcase some of the most recent studies and developments in the biomedical applications of nanocomposite hydrogels. Nanoparticles and nanostructures are added to cross-link the polymer network and add new properties to hydrogels [8]. Clay has been introduced into a polymeric solution system for cross-linking through non-covalent physical interactions between clay and the polymer chain to form flexible hydrogels [9] to be applied in an actuator. The cross-linked polymer networks are capable of reversible volume change in response to external stimuli. Magnetic nanoparticles incorporated in thermo-responsive polymer hydrogels show magneto-thermo stimuli-responsive properties for cell regulation [10]. Furthermore, when used as a biosensor, the distribution of gold nanoparticles in hydrogels influences the nanoplasmonic response. Therefore, a new strategy has been developed for nanoparticle mapping in hydrogels using electron microscopy [11].For drug delivery, protein-releasing hydrogels are widely used. DNA is a polymer material required for hydrogel formation. Du reported that aptamer-functionalized DNA hydrogels can serve as an appropriate candidate for controlled protein delivery by controlling complementary sequences as a biomolecular trigger [12].For soft tissue replacement, hydrogels are a suitable choice. Polyvinyl alcohol (PVA) has been extensively studied as an articular cartilage material [13,14]. PVA solution forms a hydrogel because the hydrogen bondin...

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Aptamer Functionalized DNA Hydrogel for Wise-Stage Controlled Protein Release

Cited by 23 publications

References 46 publications

DNA-GEL, Novel Nanomaterial for Biomedical Applications and Delivery of Bioactive Molecules

DNA-GEL, Novel Nanomaterial for Biomedical Applications and Delivery of Bioactive Molecules

Design, Bioanalytical, and Biomedical Applications of Aptamer-Based Hydrogels

Special Issue: Nanocomposite Hydrogels for Biomedical Applications

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