Aptamer-Functionalized DNA–Silver Nanocluster Nanofilm for Visual Detection and Elimination of Bacteria

Yang, Min; Xu, Chen; Zhu, Longjiao; Lin, Shenghao; Li, Chenwei; Li, Xiangyang; Huang, Kunlun; Xu, Wentao

doi:10.1021/acsami.1c05751

Cited by 64 publications

(33 citation statements)

References 43 publications

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“…The introduction of FNAs, especially aptamers, with excellent recognition capabilities, has enabled NAHs to gain special target-responsiveness [56]. An aptamer is a typical Triplex DNA as an important FNA is formed by the third strand connecting with a double-stranded DNA though the hydrogen bond.…”

Section: Target-responsive Nahsmentioning

confidence: 99%

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Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields

Zhang

Zhu

et al. 2022

IJMS

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Due to their hydrophilic, biocompatible and adjustability properties, hydrogels have received a lot of attention. The introduction of nucleic acids has made hydrogels highly stimuli-responsiveness and they have become a new generation of intelligent biomaterials. In this review, the development and utilization of smart nucleic acid hydrogels (NAHs) with a high stimulation responsiveness were elaborated systematically. We discussed NAHs with a high stimuli-responsiveness, including pure NAHs and hybrid NAHs. In particular, four stimulation factors of NAHs were described in details, including pH, ions, small molecular substances, and temperature. The research progress of nucleic acid hydrogels in biomedical applications in recent years is comprehensively discussed. Finally, the opportunities and challenges facing the future development of nucleic acid hydrogels are also discussed.

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Section: Target-responsive Nahsmentioning

confidence: 99%

Section: Target-responsive Nahsmentioning

confidence: 99%

Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields

Zhang

Zhu

et al. 2022

IJMS

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“…NCs not only possess unique fluorescent properties, but also have received extensive attention for their antibacterial properties ( Javani et al, 2016 ). Yang et al achieve a novel approach for visual detection and effective elimination of S. aureus by combing DNA-templated silver nanoclusters (DNA-AgNCs) and aptamers ( Yang et al, 2021a ). Compared with general fluorescent nanomaterials, polymer-based fluorescent nanomaterials exhibit excellent biocompatibility and biodegradability, and are easily prepared by self-polymerization ( Zhang et al, 2015c ).…”

Section: Aptasensors Based On Optical Transductionmentioning

confidence: 99%

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Chen

Lai

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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The infection of Staphylococcus aureus (S.aureus) and the spread of drug-resistant bacteria pose a serious threat to global public health. Therefore, timely, rapid and accurate detection of S. aureus is of great significance for food safety, environmental monitoring, clinical diagnosis and treatment, and prevention of drug-resistant bacteria dissemination. Traditional S. aureus detection methods such as culture identification, ELISA, PCR, MALDI-TOF-MS and sequencing, etc., have good sensitivity and specificity, but they are complex to operate, requiring professionals and expensive and complex machines. Therefore, it is still challenging to develop a fast, simple, low-cost, specific and sensitive S. aureus detection method. Recent studies have demonstrated that fast, specific, low-cost, low sample volume, automated, and portable aptasensors have been widely used for S. aureus detection and have been proposed as the most attractive alternatives to their traditional detection methods. In this review, recent advances of aptasensors based on different transducer (optical and electrochemical) for S. aureus detection have been discussed in details. Furthermore, the applications of aptasensors in point-of-care testing (POCT) have also been discussed. More and more aptasensors are combined with nanomaterials as efficient transducers and amplifiers, which appears to be the development trend in aptasensors. Finally, some significant challenges for the development and application of aptasensors are outlined.

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“…As a new type of nanomaterial, silver nanoclusters (AgNCs) possess prominent antibacterial efficacy attributed to the synergistic effect of AgNCs and Ag + . By conjugation of S. aureus aptamers to DNA-templated AgNCs, the selectivity and antibacterial effect of DNA-AgNCs were significantly enhanced in the visual monitoring and elimination of S. aureus . Obvious membrane blebbing and structural damage of the bacteria could be observed under scanning electron microscope (SEM).…”

Section: Therapeutic Aptamers Against Infectious Pathogensmentioning

confidence: 99%

“…By conjugation of S. aureus aptamers to DNAtemplated AgNCs, the selectivity and antibacterial effect of DNA-AgNCs were significantly enhanced in the visual monitoring and elimination of S. aureus. 76 Obvious membrane blebbing and structural damage of the bacteria could be observed under scanning electron microscope (SEM).…”

Section: Therapeutic Aptamers Against Infectious Pathogensmentioning

confidence: 99%

Aptamer-Based Antibacterial and Antiviral Therapy against Infectious Diseases

2021

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Nucleic acid aptamers are single-stranded DNA or RNA molecules selected in vitro that can bind to a broad range of targets with high affinity and specificity. As promising alternatives to conventional anti-infective agents, aptamers have gradually revealed their potential in the combat against infectious diseases. This article provides an overview on the state-of-art of aptamer-based antibacterial and antiviral therapeutic strategies. Diverse aptamers targeting pathogen-related components or whole pathogenic cells are summarized according to the species of microorganisms. These aptamers exhibited remarkable in vitro and/or in vivo inhibitory effect for pathogenic invasion, enzymatic activities, or viral replication, even for some highly drug-resistant strains and biofilms. Aptamer-mediated drug delivery and controlled drug release strategies are also included herein. Critical technical barriers of therapeutic aptamers are briefly discussed, followed by some future perspectives for their implementation into clinical utility.

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Aptamer-Functionalized DNA–Silver Nanocluster Nanofilm for Visual Detection and Elimination of Bacteria

Cited by 64 publications

References 43 publications

Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields

Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields

Recent Advances in Aptasensors For Rapid and Sensitive Detection of Staphylococcus Aureus

Aptamer-Based Antibacterial and Antiviral Therapy against Infectious Diseases

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