Aptamer lateral flow assays for rapid and sensitive detection of cholera toxin

Frohnmeyer, Esther; Tuschel, Nadine; Sitz, Tobias; Hermann, Cornelia; Dahl, Gregor T.; Schulz, Florian; Baeumner, Antje J.; Fischer, Markus

doi:10.1039/c8an01616j

Cited by 63 publications

(20 citation statements)

References 32 publications

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“…However, DNA aptamers also have drawbacks of lacking wide array of computational tools. An approach predicting from sequence to 3D structures of ssDNA for aptamer application was firstly reported by Jeddi et al The approach integrates 3D ssRNA models to ssDNA 3D structures and refines the resulting ssDNA 3D structure, which could faithfully predict the representative nucleic acid database, thus opening a new avenue for the integration of DNA computational analysis with aptamer-based biosensors designing [100]. Many efforts have been made to enhance the sensitivity and selectivity of biosensors based on aptamers [101], however, little attention have been focused on the improvement of the response rate of biosensor.…”

Section: Recent Advances In Detecting Toxins By Aptasensorsmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

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Marine toxins cause great harm to human health through seafood, therefore, it is urgent to exploit new marine toxins detection methods with the merits of high sensitivity and specificity, low detection limit, convenience, and high efficiency. Aptasensors have emerged to replace classical detection methods for marine toxins detection. The rapid development of molecular biological approaches, sequencing technology, material science, electronics and chemical science boost the preparation and application of aptasensors. Taken together, the aptamer-based biosensors would be the best candidate for detection of the marine toxins with the merits of high sensitivity and specificity, convenience, time-saving, relatively low cost, extremely low detection limit, and high throughput, which have reduced the detection limit of marine toxins from nM to fM. This article reviews the detection of marine toxins by aptamer-based biosensors, as well as the selection approach for the systematic evolution of ligands by exponential enrichment (SELEX), the aptamer sequences. Moreover, the newest aptasensors and the future prospective are also discussed, which would provide thereotical basis for the future development of marine toxins detection by aptasensors.Key Contribution: This article reviews systematically the toxicity of marine toxins, the development of aptarmer-based biosensors, and progress in the marine toxin detection by aptasensors.Toxins 2020, 12, 1 2 of 22 selection technology and biosensor fabrication technology offer a new solution for the detection of marine toxins with high efficiency and sensitivity [13][14][15][16][17].Marine toxins are a class of small molecular compounds mainly produced by red tide algae. In recent years, with the aggravation of environmental pollution, human deaths due to accidental ingestion of toxic shellfish are also often reported [18][19][20][21]. At present, the main methods for the detection of marine toxins include mouse bioassay (MBA) [22], enzyme-linked immunosorbent assay (ELISA) [23][24][25], high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS/MS), etc. [26][27][28]. However, these traditional methods have some drawbacks including the requirement of a technician, poor repeatability, expensive equipment, and issues related to animal ethics [22]. As a new detection technology, the biosensor is a kind of analysis systems using cell molecules and other bio-materials as sensitive elements, combined with a secondary sensor to detect a variety of chemicals by cascade amplified signal [29]. Because of their advantages of simple operation, high speed, high sensitivity, miniaturization, and easy automation, biosensors have been widely used in different fields including cell physiology [30], drug screening [31,32], food safety detection [33], disease diagnosis [34], etc.Aptamer, meaning "to fit", is a kind of oligonucleotide which can bind to target molecules with high selectivity and affinity. On account of the existence of aptamers in nature and the po...

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Section: Recent Advances In Detecting Toxins By Aptasensorsmentioning

confidence: 99%

Marine Toxins Detection by Biosensors Based on Aptamers

Wei

Liu

Zhang

et al. 2019

Toxins

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“…Molecular-based recognition elements offer significant advantages over antibodies. Antibodies have low stability at high temperatures, high production costs and variation with each subsequent batch, and a need for a constant supply of mammalian cell culture ( 44 ). In contrast, aptamers are non-immunogenic and are highly stable at various conditions maintaining their high binding affinity with the target analytes ( 45 ).…”

Section: Molecular-based Methodsmentioning

confidence: 99%

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Manhas

Quintela

2021

Front. Vet. Sci.

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Nanotechnology has gained prominence over the recent years in multiple research and application fields, including infectious diseases in healthcare, agriculture, and veterinary science. It remains an attractive and viable option for preventing, diagnosing, and treating diseases in animals and humans. The apparent efficiency of nanomaterials is due to their unique physicochemical properties and biocompatibility. With the persistence of pathogens and toxins in the poultry and livestock industries, rapid diagnostic tools are of utmost importance. Though there are many promising nanomaterials-based diagnostic tests specific to animal disease-causing agents, many have not achieved balanced sensitivity, specificity, reproducibility, and cost-effectiveness. This mini-review explores several types of nanomaterials, which provided enhancement on the sensitivity and specificity of recently reported diagnostic tools related to animal diseases. Recommendations are also provided to facilitate more targeted animal populations into the development of future diagnostic tools specifically for emerging and re-emerging animal diseases posing zoonotic risks.

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“…Complex folding allows aptamer to specifically recognize the target ( Gelinas et al, 2016 ; Zhou and Rossi, 2017 ). A broad range of targets includes organic molecules ( Umar and Kit, 2020 ), proteins ( Umar and Kit, 2020 ), nucleotides ( Yue et al, 2011 ), cancer cells ( Guizhi and Xiaoyuan, 2018 ), bacteria ( Alizadeh et al, 2018 ), toxins ( Frohnmeyer et al, 2019 ), or viruses ( Cesewski and Johnson, 2020 ). Compared with antibodies, aptamers with flexible structures and very small molecular weights can cross the blood-brain barrier ( Cheng et al, 2013 ), a physiological barrier that antibodies cannot enter, and bind to target molecules with high affinity and specificity.…”

Section: Properties Of Aptamersmentioning

confidence: 99%

Advances in Screening and Development of Therapeutic Aptamers Against Cancer Cells

Huang

et al. 2021

Front. Cell Dev. Biol.

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Cancer has become the leading cause of death in recent years. As great advances in medical treatment, emerging therapies of various cancers have been developed. Current treatments include surgery, radiotherapy, chemotherapy, immunotherapy, and targeted therapy. Aptamers are synthetic ssDNA or RNA. They can bind tightly to target molecules due to their unique tertiary structure. It is easy for aptamers to be screened, synthesized, programmed, and chemically modified. Aptamers are emerging targeted drugs that hold great potentials, called therapeutic aptamers. There are few types of therapeutic aptamers that have already been approved by the US Food and Drug Administration (FDA) for disease treatment. Now more and more therapeutic aptamers are in the stage of preclinical research or clinical trials. This review summarized the screening and development of therapeutic aptamers against different types of cancer cells.

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Aptamer lateral flow assays for rapid and sensitive detection of cholera toxin

Abstract: A GM1-liposome aptamer sandwich LFA was developed and compared with AuNP-based competitive aptamer and aptamer-antibody sandwich LFAs for cholera toxin detection.

Cited by 63 publications

References 32 publications

Marine Toxins Detection by Biosensors Based on Aptamers

Marine Toxins Detection by Biosensors Based on Aptamers

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Advances in Screening and Development of Therapeutic Aptamers Against Cancer Cells

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