Aptamer-modified metal organic frameworks for measurement of food contaminants: a review

“…The advantages of cross-reactivity can be harnessed effectively when the recognition elements and assays are thoughtfully designed for the intended application. Utilizing various MOFs, their composites with metal nanoparticles, and DNA aptamers as an immobilizer, various practices have been explored previously to reduce cross-reactivity instead of inculcating its benefits [ 16 ]. Several modifications in the aptamer immobilization methodologies prevent cross-reactive effects on HMI detection adopted by various research groups [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Layer-by-Layer Immobilization of DNA Aptamers on Ag-Incorporated Co-Succinate Metal–Organic Framework for Hg(II) Detection

Patil,

Narwade,

Sontakke

et al. 2024

Sensors

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Layer-by-layer (LbL) immobilization of DNA aptamers in the realm of electrochemical detection of heavy metal ions (HMIs) offers an enhancement in specificity, sensitivity, and low detection limits by leveraging the cross-reactivity obtained from multiple interactions between immobilized aptamers and developed material surfaces. In this research, we present a LbL approach for the immobilization of thiol- and amino-modified DNA aptamers on a Ag-incorporated cobalt-succinate metal–organic framework (MOF) (Ag@Co-Succinate) to achieve a cross-reactive effect on the electrochemical behavior of the sensor. The solvothermal method was utilized to synthesize Ag@Co-Succinate, which was also characterized through various techniques to elucidate its structure, morphology, and presence of functional groups, confirming its suitability as a host matrix for immobilizing both aptamers. The Ag@Co-Succinate aptasensor exhibited extraordinary sensitivity and selectivity towards Hg(II) ions in electrochemical detection, attributed to the unique binding properties of the immobilized aptamers. The exceptional limit of detection of 0.3 nM ensures the sensor’s suitability for trace-level Hg(II) detection in various environmental and analytical applications. Furthermore, the developed sensor demonstrated outstanding repeatability, highlighting its potential for long-term and reliable monitoring of Hg(II).

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Fluorescent aptasensor mediated with multiple ssDNA for sensitive detection of acetamiprid in vegetables based on magnetic Fe3O4/C-assisted separation

Li,

Wang,

Liu

et al. 2024

Anal Bioanal Chem

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Aptamer-modified metal organic frameworks for measurement of food contaminants: a review

Cited by 11 publications

References 180 publications

Target-triggered dual signal amplification based on HCR-enhanced nanozyme activity for the sensitive visual detection of Escherichia coli

Target-triggered dual signal amplification based on HCR-enhanced nanozyme activity for the sensitive visual detection of Escherichia coli

Layer-by-Layer Immobilization of DNA Aptamers on Ag-Incorporated Co-Succinate Metal–Organic Framework for Hg(II) Detection

Fluorescent aptasensor mediated with multiple ssDNA for sensitive detection of acetamiprid in vegetables based on magnetic Fe3O4/C-assisted separation

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