Aptamer/organometallic receptor-based and label-free sensitive electrochemical detection of amino acid via multi-pedal DNA walker/DNAzyme amplifications

Yang, Jianmei; Bi, Qian; Song, Xinmei; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.snb.2022.132876

Cited by 8 publications

(4 citation statements)

References 35 publications

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“…Therefore, the regularly lifelong monitoring of the Phe concentration is crucial for the patient’s well-being. − Recently various analytical approaches for the detection of Phe have been developed. They include a nonenzymatic method based on recognition of Phe by interaction with specific DNA-aptamers sequences, enzymatic approaches mainly based on phenylalanine ammonia lyase enzyme (PAL) and on phenylalanine dehydrogenase enzyme (PDH) assisted by redox or colorimetric mediators …”

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confidence: 99%

“…6−8 Recently various analytical approaches for the detection of Phe have been developed. They include a nonenzymatic method based on recognition of Phe by interaction with specific DNA-aptamers sequences, 9 enzymatic approaches mainly based on phenylalanine ammonia lyase enzyme (PAL) 10 and on phenylalanine dehydrogenase enzyme (PDH) assisted by redox or colorimetric mediators. 11 Great efforts have been made in the development of nanostructured materials to improve analytical performance and enable the integration of assays into miniaturized sensing devices.…”

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confidence: 99%

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Photothermal-Contrast Method Based on In Situ Gold Nanostructure Formation for Phenylalanine Detection in Human Blood

Maugeri

Messina

Ruggieri

et al. 2023

ACS Appl. Nano Mater.

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An innovative approach based on photothermal contrast to enhance analytical sensitivity of enzymatic phenylalanine detection at a solid surface was demonstrated. Gold nanoparticles in situ produced by the reduced form of nicotinamide adenine dinucleotide, which is generated by an enzymatic reaction, exhibit an excellent photothermal effect upon green-light stimuli. The enzymatic reaction and the nanoparticles process formation were optically investigated. A sensing range of 10−1000 μM with a detection limit of 3.6 μM for phenylalanine detection were demonstrated. The testing with blood samples collected from phenylketonuria patients together with concurrent comparison through tandem mass spectrometry have confirmed the good analytical performances and the robustness and utility of the proposed photothermal-contrast approach for further integration on clinical devices.

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confidence: 99%

Photothermal-Contrast Method Based on In Situ Gold Nanostructure Formation for Phenylalanine Detection in Human Blood

Maugeri

Messina

Ruggieri

et al. 2023

ACS Appl. Nano Mater.

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“…Recently, an alternative approach based on the aptamer/organometallic receptor was reported by Yang and colleagues for the electrochemical determination of phenylalanine. A linear range of 10–260 µM and an LoD of 1.03 µM were declared by the authors Yang et al (2023) .…”

Section: Resultsmentioning

confidence: 99%

A highly sensitive colorimetric approach based on tris (bipyridine) Ruthenium (II/III) mediator for the enzymatic detection of phenylalanine

et al. 2023

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The accurate monitoring of phenylalanine concentration plays a prominent role in the treatment of phenylketonuria (PKU). In this study, we present an enzymatic assay based on Phenylalanine Dehydrogenase/NAD+ and tris (bipyridine) Ruthenium (II/III) as a colorimetric mediator for the detection of Phenylalanine concentration. The amount of amino acid was quantitatively recognized by optical absorption measurements at 452 nm through the conversion of Ru (byp)33+ to Ru (byp)32+, which is induced by the neoformed NADH. A detection limit of 0.33 µM, a limit of quantification of 1.01 µM, and a sensitivity of 36.6 a.u nM−1 were obtained. The proposed method was successfully tested using biological specimens from patients affected by hyperphenylalaninemia. The proposed enzymatic assay showed a high selectivity, making it a promising alternative for the development of versatile assays for the detection of phenylalanine in diluted serums.

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“…During the last years, different enzymatic and nonenzymatic approaches for Phe recognition have been reported in the literature. Most of them are based on colorimetric − and electric detection, − and some of these were successfully integrated into miniaturized devices, , including wearable sensors . Despite the declared good analytical performances, they appear difficult to be developed in a fully integrated platform for commercial purposes, due to different constraints such as high cost analysis, laborious procedures, and limited usability.…”

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confidence: 99%

Fully Integrated Point-of-Care Platform for the Self-Monitoring of Phenylalanine in Finger-Prick Blood

Messina,

Maugeri,

Spoto

et al. 2023

ACS Sens.

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Development of point-of-care platforms combining reliability and ease of use is a challenge for the evolution of sensing in healthcare technologies. Here, we report the development and testing of a fully integrated enzymatic colorimetric assay for the sensing of phenylalanine in blood samples from phenylketonuria patients. The platform works with a customized mobile app for data acquisition and visualization and comprises an electronic system and a disposable sensor. The sensing approach is based on specific enzymatic phenylalanine recognition, and the optical transduction method is based on in situ gold nanostructure formation. The phenylketonuria (PKU) smart sensor platform is conceived to perform self-monitoring on phenylalanine levels and real-time therapy tuning, thanks to the direct connection with clinicians. Validation of the technologies with a population of patients affected by PKU, together with the concurrent validation of the platform through centralized laboratories, has confirmed the good analytical performances in terms of sensitivity and specificity, robustness, and utility for phenylalanine sensing. The self-monitoring of phenylalanine for the daily identification of abnormal health conditions could facilitate rapid therapy tuning, improving the wellness of PKU patients.

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Aptamer/organometallic receptor-based and label-free sensitive electrochemical detection of amino acid via multi-pedal DNA walker/DNAzyme amplifications

Cited by 8 publications

References 35 publications

Photothermal-Contrast Method Based on In Situ Gold Nanostructure Formation for Phenylalanine Detection in Human Blood

Photothermal-Contrast Method Based on In Situ Gold Nanostructure Formation for Phenylalanine Detection in Human Blood

A highly sensitive colorimetric approach based on tris (bipyridine) Ruthenium (II/III) mediator for the enzymatic detection of phenylalanine

Fully Integrated Point-of-Care Platform for the Self-Monitoring of Phenylalanine in Finger-Prick Blood

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