Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples

Wu, Xueyuan; Hu, Jun; Zhu, Baolin; Lü, Lin; Huang, Xiangdong; Pang, Dai‐Wen

doi:10.1016/j.chroma.2011.08.045

Cited by 89 publications

(46 citation statements)

References 37 publications

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“…[64], where magnetic nanospheres (MNS) were used as solid support for aptamer immobilization. Nano-magnetic materials are very suitable for sample preparation, because they can be easily collected by a magnetic field with no need for centrifugation or filtration after extraction.…”

Section: Aptamer-based Affinity Columns For Selective Retention Ofmentioning

confidence: 99%

Aptamers: A Promising Tool for Ochratoxin A Detection in Food Analysis

Rhouati

Yang

Hayat

et al. 2013

Toxins

118

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The contamination of food and feed by mycotoxins has become an increasingly serious problem. Mycotoxins represent a major risk to human and animal health, as well as economics. Herein, we focus on Ochratoxin A (OTA), which is one of the most common mycotoxins contaminating feed and foodstuffs. OTA is a secondary metabolite produced by various Aspergillus and Penicillium strains. Upon ingestion, OTA has a number of acute and chronic toxic effects. It is nephrotoxic, teratogenic, immunosuppressive, and carcinogenic (group 2B). As a consequence, some regulatory limits have been introduced on the levels of OTA in several commodities. The toxic nature of OTA demands highly sensitive and selective monitoring techniques to protect human and animal health. As alternative to traditional analytical techniques, biochemical methods for OTA analysis have attained great interest in the last few decades. They are mainly based on the integration of antibodies or aptamers as biorecognition elements in sensing platforms. However, aptamers have gained more attention in affinity-based assays because of their high affinity, specificity, stability, and their easy chemical synthesis. In this brief review, we present an overview of aptamer-based assays and their applications in OTA purification and detection, appeared in the literature in the last five years.

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Section: Aptamer-based Affinity Columns For Selective Retention Ofmentioning

confidence: 99%

Aptamers: A Promising Tool for Ochratoxin A Detection in Food Analysis

Rhouati

Yang

Hayat

et al. 2013

Toxins

118

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“…Recoveries of OTA from 67% to 90% were found in spiked wheat flour, coffee and cereal products. HPLC was coupled to this capture method for quantification [73]. Similarly, aptamer-modified glass microbeads selectively isolated 17β-estradiol in the presence of other hormones and antibiotics and this enrichment was coupled to the chromatographic determination of the hormone in spiked river water [74].…”

Section: Other Growing Food Concernsmentioning

confidence: 99%

Aptamer-Based Analysis: A Promising Alternative for Food Safety Control

Amaya-González

de‐los‐Santos‐Álvarez

Miranda‐Ordieres

et al. 2013

Sensors

122

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Ensuring food safety is nowadays a top priority of authorities and professional players in the food supply chain. One of the key challenges to determine the safety of food and guarantee a high level of consumer protection is the availability of fast, sensitive and reliable analytical methods to identify specific hazards associated to food before they become a health problem. The limitations of existing methods have encouraged the development of new technologies, among them biosensors. Success in biosensor design depends largely on the development of novel receptors with enhanced affinity to the target, while being stable and economical. Aptamers fulfill these characteristics, and thus have surfaced as promising alternatives to natural receptors. This Review describes analytical strategies developed so far using aptamers for the control of pathogens, allergens, adulterants, toxins and other forbidden contaminants to ensure food safety. The main progresses to date are presented, highlighting potential prospects for the future.

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“…In recent years, magnetic solid-phase extraction (MSPE) has been used to determine mycotoxins (Wu et al 2011;Mashhadizadeh et al 2013;Hashemi et al 2014;McCullum et al 2014). In MSPE, magnetic nanoparticles (MNPs) are dispersed into the sample solution to extract analytes and the adsorbent together with the analytes can be conveniently separated from the solution by applying an external magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Quantification of mycotoxins in vegetable oil by UPLC-MS/MS after magnetic solid-phase extraction

Zhao

Wan

Bai

et al. 2017

Food Additives & Contaminants: Part A

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The detection of mycotoxin contamination in foodstuffs is highly significant for public health. Herein we report an analytical method based on magnetic solid-phase extraction (MSPE) and UPLC-MS/MS for the simultaneous determination of mycotoxins, including fumonisins B 1 (FB 1 ), zearalenone (ZON) and ochratoxin A (OTA), in vegetable oil. Magnetic nanoparticles coated with double layers of silicon dioxide were synthesised and found to be an effective MSPE adsorbent for mycotoxins. The proposed MSPE procedure serves not only for sample clean-up but also for mycotoxin enrichment that enhances greatly the assay's sensitivity. Under the selected MSPE conditions, linear matrix-matched calibration curves were obtained for mycotoxins in a concentration range from 0.178 to 625 μg kg -1. The limits of detection were 0.210 μg kg -1 for FB 1 , 0.0800 μg kg -1 for OTA and 1.03 μg kg -1 for ZON. The proposed MSPE UPLC-MS/MS method was applied for the determination of mycotoxins in vegetable oil samples, including maize oil, rapeseed oil and soybean oil. ZON was detected in a maize oil at 101 μg kg -1 , which is below the European Union limit of 200 μg kg -1 in foodstuffs.ARTICLE HISTORY

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Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples

Cited by 89 publications

References 37 publications

Aptamers: A Promising Tool for Ochratoxin A Detection in Food Analysis

Aptamers: A Promising Tool for Ochratoxin A Detection in Food Analysis

Aptamer-Based Analysis: A Promising Alternative for Food Safety Control

Quantification of mycotoxins in vegetable oil by UPLC-MS/MS after magnetic solid-phase extraction

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