Aptamer/thrombin/aptamer-AuNPs sandwich enhanced surface plasmon resonance sensor for the detection of subnanomolar thrombin

Bai, Yunfeng; Feng, Feng; Zhao, Lu; Wang, Chunyu; Wang, Haiyan; Tian, Mingliang; Qin, Jun; Duan, Ying; He, Xiaoxiao

doi:10.1016/j.bios.2013.02.004

Cited by 157 publications

(74 citation statements)

References 46 publications

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“…It was lower than those obtained from other reported assays (Table 1), such as surface plasmon resonance (50 nM), 22 graphene oxide-aptamer sensor (4.8 pM), 18 paper analytical device (16 nM), 23 and quartz crystal microbalance (0.1nM), 24 graphene nanocomposites (0.01 nM), 25 and electrochemical sandwich assay (1.5 pM). 26 It was also lower than that obtained from similar mechanisms using other sensors, such as graphene oxide sensor (0.9 pM), 16 strip biosensor (4.9 pM), 27 and AuNP sensor (0.31 nM).…”

Section: Optimization Of Experiments Conditions For Exonuclease Coassicontrasting

confidence: 60%

Highly sensitive protein detection via covalently linked aptamer to MoS<sub>2</sub> and exonuclease-assisted amplification strategy

Gao¹,

Li²,

Deng³

et al. 2017

IJN

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Molybdenum disulfide (MoS 2 ) has shown highly attractive superiority as a platform for sensing. However, DNA physisorption on the surface of MoS 2 was susceptible to nonspecific probe displacement and false-positive signals. To solve these problems, we have developed a novel MoS 2 –aptamer nanosheet biosensor for detecting thrombin using a covalently linked aptamer to the MoS 2 nanosheet. Ten percent Tween 80 was used to prevent thrombin from nonspecific binding and to rapidly form thiol-DNA/gold nanoparticle (AuNP) conjugates. Furthermore, an MoS 2 and exonuclease coassisted signal amplification strategy was developed to improve the detection limit for thrombin. We used the hybridization of the aptamer molecules and the matched strand with a 5′ terminal thiol to immobilize the aptamer molecules on the surface of AuNPs in AuNPs@MoS 2 nanocomposites. Exonuclease digested the single-strand aptamer and released the thrombin, which was then detected in the next recycle. With the coassisted amplification strategy, a 6 fM detection limit was achieved, showing that this method has higher sensitivity than most reported methods for thrombin detection. The results presented in this work show that this method of covalently attaching the aptamer and using the coassisted amplification is a promising technique for the detection of protein in medical diagnostics.

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Section: Optimization Of Experiments Conditions For Exonuclease Coassicontrasting

confidence: 60%

Highly sensitive protein detection via covalently linked aptamer to MoS<sub>2</sub> and exonuclease-assisted amplification strategy

Gao¹,

Li²,

Deng³

et al. 2017

IJN

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“…In recent years, many approaches for detecting TB have been developed based on the specific binding between TB and the TB aptamer (TBA), such as electrochemiluminescence , surface plasmon resonance (Bai et al, 2013), colorimetry (Wang et al, 2008), electrochemistry (Jiang et al, 2013) and fluorescence (Wang et al, 2011). Comparatively, electrochemical aptamer-based sensor (aptasensor) with the advantages of low cost, simple device portability and high sensitivity (Zhao et al, 2011;Liu et al, 2012), has attracted distinct attention in TB determination.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical aptasensor for thrombin using synergetic catalysis of enzyme and porous Au@Pd core–shell nanostructures for signal amplification

Yuan

et al. 2015

Biosensors and Bioelectronics

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“…The limit of detection was estimated to be 0.5 nM based on S/N of 3, the sensitivity was comparable or better than that in the reported thrombin detection. [44][45][46][47] To test the specificity of this assay toward thrombin, Bovine Serum Albumin (BSA), lysozyme, myoglobin, papain and HBsAg were analyzed in the same binding buffer. Fig.…”

Section: Detection Of Thrombinmentioning

confidence: 99%

Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin

Yang

Liang

et al. 2015

Analyst

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Rapid and sensitive detection of thrombin has very important significance in clinical diagnosis. In this work, bare magnetic iron oxide nanoparticles (magnetic nanoparticles) without any modification were used as fluorescence quenchers. In the absence of thrombin, a fluorescent dye (CY3) labeled thrombin aptamer (named CY3-aptamer) was adsorbed on the surface of magnetic nanoparticles through interaction between a phosphate backbone of the CY3-aptamer and hydroxyl groups on the bare magnetic nanoparticles in binding solution, leading to fluorescence quenching. Once thrombin was introduced, the CY3-aptamer formed a G-quartet structure and combined with thrombin, which resulted in the CY3-aptamer being separated from the magnetic nanoparticles and restoration of fluorescence. This proposed assay took advantage of binding affinity between the CY3-aptamer and thrombin for specificity, and bare magnetic nanoparticles for fluorescence quenching. The fluorescence signal had a good linear relationship with thrombin concentration in the range of 1-60 nM, and the limit of detection for thrombin was estimated as low as 0.5 nM. Furthermore, this method could be applied for other target detection using the corresponding fluorescence labeled aptamer.

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Aptamer/thrombin/aptamer-AuNPs sandwich enhanced surface plasmon resonance sensor for the detection of subnanomolar thrombin

Cited by 157 publications

References 46 publications

Highly sensitive protein detection via covalently linked aptamer to MoS<sub>2</sub> and exonuclease-assisted amplification strategy

Highly sensitive protein detection via covalently linked aptamer to MoS<sub>2</sub> and exonuclease-assisted amplification strategy

An electrochemical aptasensor for thrombin using synergetic catalysis of enzyme and porous Au@Pd core–shell nanostructures for signal amplification

Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin

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