Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate

Wang, Pengjuan; Wan, Ying; Aldalbahi, Ali; Deng, Shengyuan; Su, Yan; Chen, Fan; Yang, Shulin

doi:10.1007/s11426-015-5488-5

Cited by 79 publications

(35 citation statements)

References 46 publications

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“…This observation was again contradicted with the majority of literature. 36 , 40 , 41 However, two previous studies showed similar AuNPs aggregation patterns observed in this current study. 42 , 43 Chavez et al reported an AuNPs colorimetric detection assay utilizing the riboflavin binding aptamer (RBA).…”

Section: Results and Discussionsupporting

confidence: 87%

In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine

et al. 2018

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Atrazine is an herbicide that is widely used in crop production at about 70 million pounds per year in the United States. Its widespread use has led to contamination of groundwater and other aquatic systems. It has resulted in many serious environmental and human health issues. This study focuses on the identification and characterization of a single-stranded DNA (ssDNA) aptamer that binds to atrazine. In this study, a variation of the systematic evolution of ligands by exponential enrichment (SELEX) process was used to identify an aptamer, which binds to atrazine with high affinity and specificity. This SELEX focused on inducing the aptamer’s ability to change conformation upon binding to atrazine, and stringent negative target selections. After 12 rounds of in vitro selection, the ssDNA aptamer candidate R12.45 was chosen and truncated to obtain a 46-base sequence. The binding affinity, specificity, and structural characteristics of this truncated candidate was investigated by using isothermal titration calorimetry, circular dichroism (CD) analysis, SYBR Green I (SG) fluorescence displacement assays, and gold nanoparticles (AuNPs) colorimetric assays. The truncated R12.45 candidate aptamer bound to atrazine with high affinity (Kd = 3.7 nM) and displayed low cross-binding activities on structurally related herbicides. In addition, CD analysis data indicated a target induced structural stabilization. Finally, SG assays and AuNPs assays showed nonconventional binding activities between the truncated R12.45 aptamer candidate and atrazine, which warrants future studies.

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Section: Results and Discussionsupporting

confidence: 87%

In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine

et al. 2018

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“…Graphene-based composites as filler materials where graphene is used as a base to disperse in or incorporation of other nanomaterials, such as metal nanostructures [22][23][24], semiconductor nanostructures [25][26][27], and polymers [28,29]. Chemical reduction, hydrothermal method, microwave-assisted growth, solution mixing, in situ growth are often used to prepare graphene-based composites [30].…”

Section: Graphene-based Compositesmentioning

confidence: 99%

Graphene-based nanomaterials in biosystems

Lü

Wang

et al. 2018

Nano Res.

Self Cite

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Graphene-based nanomaterials have emerged as a novel type of materials with exceptional physicochemical properties and numerous applications in various areas. In this review, we summarize recent advances in studying interactions between graphene and biosystems. We first provide a brief introduction on graphene and its derivatives, and then discuss on the toxicology and biocompatibility of graphene, including the extracellular interactions between graphene and biomacromolecules, cellular studies of graphene, and in vivo toxicological effects. Next, we focus on various graphene-based practical applications in antibacterial materials, wound addressing, drug delivery, and water purification. We finally present perspectives on challenges and future developments in these exciting fields.

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“…The aptamer showed high selectivity towards omethoate, resulting in the disconnection of aptamer molecules from AuNPs and in their aggregation. Using the OP-binding aptamer and target-induced colour changes in AuNPs, this biosensor showed a good linearity between 0.1 μM and 10 μM, with LOD of 0.1 μM [ 11 ]. A similar approach was developed for the colorimetric detection of malathion employing aptamer, cationic peptide and unmodified gold nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos

Selvolini

Băjan

Hosu

et al. 2018

Sensors

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In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen aptamer sequence, was immobilised on gold nanoparticles/polyaniline composite film-modified electrodes (AuNPs/PANI/GSPE). Different profenofos solutions containing a fixed amount of the biotinylated DNA aptamer were dropped onto the realized aptasensors. The hybridisation reaction was measured using a streptavidin-alkaline phosphatase enzyme conjugate, which catalyses the hydrolysis of 1-naphthyl -phosphate. The 1-naphtol enzymatic product was detected by means of differential pulse voltammetry (DPV). The aptasensor showed itself to work as a signal off sensor, according to the competitive format used. A dose response curve was obtained between 0.10 μM and 10 μM with a detection limit of 0.27 μM.

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Aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate

Cited by 79 publications

References 46 publications

In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine

In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine

Graphene-based nanomaterials in biosystems

DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos

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