Aptameric biosensor for the sensitive detection of major shrimp allergen, tropomyosin

Chinnappan, Raja; Rahamn, Anas Abdel; AlZabn, Razan; Kamath, Sandip D.; Lopata, Andreas L.; Abu‐Salah, Khalid M.; Zourob, Mohammed

doi:10.1016/j.foodchem.2019.126133

Cited by 73 publications

(31 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The target of interest can range from small molecules such as the mycotoxins aflatoxin B1 [ 60 ] and ochratoxin A [ 61 ], bisphenol A [ 62 ], the antibiotic tobramycin [ 54 ] and the marine toxin okadaic acid [ 63 ], to larger entities such as lipopolysaccharides [ 64 ] and the cyclic heptapeptide microcystin-LR (a hepatotoxin produced by cyanobacteria) [ 65 ]. Furthermore, proteins such as tropomyosin (a major shrimp allergen) [ 66 ] and glycated hemoglobin [ 67 ] or even whole bacteria such as Salmonella enteritidis [ 68 ] can be measured using this approach. A final example of a biosensor employing truncated aptamers is the surface-enhanced Raman spectroscopy-based aptasensors for the detection of the EpCaM protein (a cancer biomarker).…”

Section: Strategies For Improving Binding Affinity Through Chemicamentioning

confidence: 99%

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Elskens

Madder

2020

IJMS

116

View full text Add to dashboard Cite

Aptamers are short single stranded DNA or RNA oligonucleotides that can recognize analytes with extraordinary target selectivity and affinity. Despite their promising properties and diagnostic potential, the number of commercial applications remains scarce. In order to endow them with novel recognition motifs and enhanced properties, chemical modification of aptamers has been pursued. This review focuses on chemical modifications, aimed at increasing the binding affinity for the aptamer’s target either in a non-covalent or covalent fashion, hereby improving their application potential in a diagnostic context. An overview of current methodologies will be given, thereby distinguishing between pre- and post-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) modifications.

show abstract

Section: Strategies For Improving Binding Affinity Through Chemicamentioning

confidence: 99%

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Elskens

Madder

2020

IJMS

116

View full text Add to dashboard Cite

show abstract

“…After the addition of TM, fluorescein-labeled aptamers can be released from the GO surface and form aptamers-TM complex with the fluorescence recovery. Similarly, the TM concentration was analyzed through the fluorescence intensity with a LOD of 2 nM in 30 min (Chinnappan et al, 2020). Weng et al (2016) established a microfluidic system incorporated with a QDs-aptamer functionalized GO nanobiosensor to detect the major allergen within 10 min in peanuts.…”

Section: Detection Of Food Allergens By Fluorescence Aptamer Sensorsmentioning

confidence: 99%

“…After the addition of TM, fluorescein‐labeled aptamers can be released from the GO surface and form aptamers‐TM complex with the fluorescence recovery. Similarly, the TM concentration was analyzed through the fluorescence intensity with a LOD of 2 nM in 30 min (Chinnappan et al., 2020). Weng et al.…”

Section: Fluorescence Aptamer Sensorsmentioning

confidence: 99%

Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors

Qian

Zhou

et al. 2020

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Food allergies are global health issue with an increasing prevalence that affect food safety; hence, food allergen detection, labeling, and management are considered to be important priorities in the food industry. In this critical review, we provide a comprehensive overview of several fluorescence-based platforms based on different biorecognition ligands, such as antibodies, DNA, aptamers, and cells, for food allergen quantification. Traditional analytical methods are generally unsuitable for food manufacturers to accomplish the real-time identification of food allergens in food products. Therefore, it is important to develop simple, rapid, inexpensive, accurate, and sensitive methods to improve user accessibility. A fluorescence-based quantitative platform provides an excellent detection platform for food allergens because of its high sensitivity. This review summarizes the traditional antibody-based fluorescent techniques for food allergen detection, such as the time-resolved fluoroimmunoassay , immunofluorescence imaging, fluorescence enzyme-linked immune sorbent assay, flow injection fluoroimmunoassay, and fluorescence immunosensors. However, these methods suffer from disadvantages such as the significant rate of false-positive and false-negative results due to antibody cross-reactivity with nontarget food components in the complex food matrix and epitope degradation during food processing. Hence, different types of fluorescence-based immunoassays are suitable for standardization and quantification of allergens in fresh foods. In addition, we summarize new fluorescence-based quantitative platforms, including fluorescence genosensors, fluorescence cell sensors, and fluorescence aptamer sensors. With the advantages of high sensitivity and simple operation, fluorescence biosensors will have great potential in the future and could provide portable methods for multiallergen real-time detection in complex food systems.

show abstract

“… Blidar et al (2019) 38 ssDNA aptamer Aptamer based enzyme immunoassay was developed for the determination of enroflaxacin Ni et al (2014) 39 ssDNA aptamer Developed an aptameric biosensor for the sensitive detection of tropomyosin allergen. Chinnappan et al (2020b) Others 40 Self-quenched probe based strategy Sensitive detection of Pseudorabies virus Shen et al (2018) 41 Mannose-modified fluorescent carbon quantum dots (Man–CQDs) Detection of bacteria using novel dual recognition strategy Cheng et al (2016) 42 NH 2 based aptamer Impedimetric aptasensor for ultrasensitive detection of Pseudomonas aeruginosa Roushani et al (2019) 43 44 Biotin tagged aptamer Mastitis detection Ashley and Li (2013) 45 Colorimetric biosensor Detection of plasmin as biomarker in case of mastitis Chinnappan et al (2017) 46 Colorimetric aptasensor Detection of E. coli strains Wu et al (2012) 47 Fluroscent probe system Quantum dot-based aptamer beacon for tumor cells. Hwang et al (2016) 48 Electrochemical biosensor Direct detection of E. coli O111.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-based diagnostic and therapeutic approaches in animals: Current potential and challenges

Devi

Sharma

Ahmed

et al. 2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Fast and precise diagnosis of infectious and non-infectious animal diseases and their targeted treatments are of utmost importance for their clinical management. The existing biochemical, serological and molecular methods of disease diagnosis need improvement in their specificity, sensitivity and cost and, are generally not amenable for being used as points-of-care (POC) device. Further, with dramatic changes in environment and farm management practices, one should also arm ourselves and prepare for emerging and re-emerging animal diseases such as cancer, prion diseases, COVID-19, influenza etc. Aptamer – oligonucleotide or short peptides that can specifically bind to target molecules – have increasingly become popular in developing biosensors for sensitive detection of analytes, pathogens (bacteria, virus, fungus, prions), drug residues, toxins and, cancerous cells. They have also been proven successful in the cellular delivery of drugs and targeted therapy of infectious diseases and physiological disorders. However, the in vivo application of aptamer-mediated biosensing and therapy in animals has been limited. This paper reviews the existing reports on the application of aptamer-based biosensors and targeted therapy in animals. It also dissects the various modifications to aptamers that were found to be successful in in vivo application of the aptamers in diagnostics and therapeutics. Finally, it also highlights major challenges and future directions in the application of aptamers in the field of veterinary medicine.

show abstract

Aptameric biosensor for the sensitive detection of major shrimp allergen, tropomyosin

Cited by 73 publications

References 42 publications

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects

Fluorescence‐based quantitative platform for ultrasensitive food allergen detection: From immunoassays to DNA sensors

Aptamer-based diagnostic and therapeutic approaches in animals: Current potential and challenges

Contact Info

Product

Resources

About