Aptamers as Molecular Recognition Elements in Chromatographic Separations

Peptide-based affinity media for solid-phase extraction of Ochratoxin A from wine samples: Effect of the solid support on binding properties

“…Therefore, over the last few decades, many efforts are being underway to replace antibodies with synthetic ligands provided with selectivity, high load capacity, chemical resistance and cost-effectiveness. [2][3][4][5] Peptides binding specific targets and selected by screening of combinatorial libraries have shown great potential as antibody-mimicking affinity ligands because of their versatile chemical and physical properties in addition to well-known synthetic approaches. [6][7] In the past, our research group has developed oligopeptide sequences with binding properties towards targets as estrogens [8], mycotoxins [9,10] and recombinant proteins [11].…”

A FACS‐Based Approach to Engineering Artificial Riboswitches

“…[1] Indeed, nucleic acid enzymes (ribozymes and deoxyribozymes) and receptors (aptamers) have emerged as major players in the era of synthetic biology, and can be applied in such disciplines as biosensing, [2,3] A C H T U N G T R E N N U N G inity purification, [4,5] nanodevices [6,7] and therapeutic development. [8,9] The success in isolating these elements can be largely attributed to the methods used to select for molecules that carry out a specific function from extremely large, random sequence libraries.…”

Aptamers and molecularly imprinted polymers as artificial biomimetic receptors in affinity capillary electrophoresis and electrochromatography

“…Experimental conditions can be easily changed in order to control aptamer structures on the solid support and to tune their binding properties. For all these peculiarities the aptamers are useful molecular recognition elements for applications in chromatography [60].…”