Aptamers coupled to nanoparticles in the diagnosis and treatment of microbial infections

Gutiérrez-Santana, Juan Carlos; Toscano-Garibay, Julia Dolores; López-López, Marisol; Coria-Jiménez, Victor Rafael

doi:10.1016/j.eimce.2020.05.001

Cited by 5 publications

(8 citation statements)

References 48 publications

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“…Moreover, aptamers are a special kind of targeting biomolecules, possessing excellent physicochemical features for superior infectious disease diagnosis and treatment [ 75 ]. Precisely, they are short single-stranded nucleic acids or peptides with defined three-dimensional structures and the ability to recognize and bind targets with high affinity and specificity [ 75 , 79 , 80 , 81 , 82 ]. Aptamers can discriminate between molecules structurally different with only one group or even enantiomers, establishing dissociation constants in the picomolar to nanomolar range for high molecular weight targets nanomolar to the micromolar range for low molecular weight targets [ 81 ].…”

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

“…Precisely, they are short single-stranded nucleic acids or peptides with defined three-dimensional structures and the ability to recognize and bind targets with high affinity and specificity [ 75 , 79 , 80 , 81 , 82 ]. Aptamers can discriminate between molecules structurally different with only one group or even enantiomers, establishing dissociation constants in the picomolar to nanomolar range for high molecular weight targets nanomolar to the micromolar range for low molecular weight targets [ 81 ]. They are generally obtained through a process of in vitro selection, following the methodology described by Tuerk and Gold in 1990 of The Systematic Evolution of Ligands by Exponential Enrichment (SELEX) [ 75 , 79 , 80 , 81 , 82 ].…”

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

“…Aptamers can discriminate between molecules structurally different with only one group or even enantiomers, establishing dissociation constants in the picomolar to nanomolar range for high molecular weight targets nanomolar to the micromolar range for low molecular weight targets [ 81 ]. They are generally obtained through a process of in vitro selection, following the methodology described by Tuerk and Gold in 1990 of The Systematic Evolution of Ligands by Exponential Enrichment (SELEX) [ 75 , 79 , 80 , 81 , 82 ]. The SELEX scheme involves three main steps, namely library and target incubation, aptamer–target complexes separation from unbound oligonucleotides, and bound molecules amplification [ 83 ].…”

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

“…The SELEX scheme involves three main steps, namely library and target incubation, aptamer–target complexes separation from unbound oligonucleotides, and bound molecules amplification [ 83 ]. Their small size, low molecular weight, stability under a variety of conditions, and lack of toxicity have allowed for an efficient nanoparticle functionalization for numerous active targeted delivery applications [ 79 , 80 , 81 ]. Moreover, they are a potential alternative for the use of antibodies, as they are able to overcome their limitations, such as higher temperature stability [ 80 ].…”

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

“…Moreover, they are a potential alternative for the use of antibodies, as they are able to overcome their limitations, such as higher temperature stability [ 80 ]. Therefore, aptamer–nanoparticle systems’ potential relies on the increase of targeting interactions due to a higher aptamer density onto the surface of the nanoparticles while being protected from the nuclease digestion [ 81 ].…”

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

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Polymeric Nanoparticles for Antimicrobial Therapies: An up-to-date Overview

et al. 2021

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Despite the many advancements in the pharmaceutical and medical fields and the development of numerous antimicrobial drugs aimed to suppress and destroy pathogenic microorganisms, infectious diseases still represent a major health threat affecting millions of lives daily. In addition to the limitations of antimicrobial drugs associated with low transportation rate, water solubility, oral bioavailability and stability, inefficient drug targeting, considerable toxicity, and limited patient compliance, the major cause for their inefficiency is the antimicrobial resistance of microorganisms. In this context, the risk of a pre-antibiotic era is a real possibility. For this reason, the research focus has shifted toward the discovery and development of novel and alternative antimicrobial agents that could overcome the challenges associated with conventional drugs. Nanotechnology is a possible alternative, as there is significant evidence of the broad-spectrum antimicrobial activity of nanomaterials and nanoparticles in particular. Moreover, owing to their considerable advantages regarding their efficient cargo dissolving, entrapment, encapsulation, or surface attachment, the possibility of forming antimicrobial groups for specific targeting and destruction, biocompatibility and biodegradability, low toxicity, and synergistic therapy, polymeric nanoparticles have received considerable attention as potential antimicrobial drug delivery agents. In this context, the aim of this paper is to provide an up-to-date overview of the most recent studies investigating polymeric nanoparticles designed for antimicrobial therapies, describing both their targeting strategies and their effects.

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Section: Microbial Targeting Strategiesmentioning

confidence: 99%

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

See 3 more Smart Citations

Polymeric Nanoparticles for Antimicrobial Therapies: An up-to-date Overview

et al. 2021

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Recent advances in targeted antibacterial therapy basing on nanomaterials

2023

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Bacterial infection has become one of the leading causes of death worldwide, particularly in low-income countries. Despite the fact that antibiotics have provided successful management in bacterial infections, the long-term overconsumption and abuse of antibiotics has contributed to the emergence of multidrug resistant bacteria. To address this challenge, nanomaterials with intrinsic antibacterial properties or that serve as drug carriers have been substantially developed as an alternative to fight against bacterial infection. Systematically and deeply understanding the antibacterial mechanisms of nanomaterials is extremely important for designing new therapeutics. Recently, nanomaterials-mediated targeted bacteria depletion in either a passive or active manner is one of the most promising approaches for antibacterial treatment by increasing local concentration around bacterial cells to enhance inhibitory activity and reduce side effects. Passive targeting approach is widely explored by searching nanomaterialbased alternatives to antibiotics, while active targeting strategy relies on biomimetic or biomolecular surface feature that can selectively recognize targeted bacteria. In this review article, we summarize the recent developments in the field of targeted antibacterial therapy based on nanomaterials, which will promote more innovative thinking focusing on the treatment of multidrug-resistant bacteria.

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