Aptamers in biomedicine: Selection strategies and recent advances

Ștefan, Geanina; Hosu, Oana; Wael, Karolien De; Lobo-Castañón, Marı́a Jesús; Cristea, Cécilia

doi:10.1016/j.electacta.2021.137994

Cited by 75 publications

(49 citation statements)

References 244 publications

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“…Aptamers, which are also known as "chemical antibodies," are DNA or RNA fragments screened via systematic evolution of ligands by exponential enrichment technology. ey can specifically bind to target molecules through hydrogen bonding, electrostatic interactions, and conformational complementation to achieve enrichment and separation with high specificity, high affinity, and easy chemical synthesis and modification [105]. Compared with ICA, aptamers have a wider range of applications because they are more stable and have a better reproducibility.…”

Section: Functional Integrationmentioning

confidence: 99%

A Review of Detection of Antibiotic Residues in Food by Surface-Enhanced Raman Spectroscopy

Liang

Peng

et al. 2021

Bioinorganic Chemistry and Applications

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Antibiotics, as veterinary drugs, have made extremely important contributions to disease prevention and treatment in the animal breeding industry. However, the accumulation of antibiotics in animal food due to their overuse during animal feeding is a frequent occurrence, which in turn would cause serious harm to public health when they are consumed by humans. Antibiotic residues in food have become one of the central issues in global food safety. As a safety measure, rapid and effective analytical approaches for detecting these residues must be implemented to prevent contaminated products from reaching the consumers. Traditional analytical methods, such as liquid chromatography, liquid chromatography mass spectrometry, and capillary electrophoresis, involve time-consuming sample preparation and complicated operation and require expensive instrumentation. By comparison, surface-enhanced Raman spectroscopy (SERS) has excellent sensitivity and remarkably enhanced target recognition. Thus, SERS has become a promising alternative analytical method for detecting antibiotic residues, as it can provide an ultrasensitive fingerprint spectrum for the rapid and noninvasive detection of trace analytes. In this study, we comprehensively review the recent progress and advances that have been achieved in the use of SERS in antibiotic residue detection. We introduce and discuss the basic principles of SERS. We then present the prospects and challenges in the use of SERS in the detection of antibiotics in food. Finally, we summarize and discuss the current problems and future trends in the detection of antibiotics in food.

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Section: Functional Integrationmentioning

confidence: 99%

A Review of Detection of Antibiotic Residues in Food by Surface-Enhanced Raman Spectroscopy

Liang

Peng

et al. 2021

Bioinorganic Chemistry and Applications

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“…It was reported that the selective recognition of targets is determined by their interaction with nucleotides via hydrogen bonding, π-stacking, van der Waals and electrostatic forces [ 46 , 48 , 49 ]. The strength of the interaction leading to aptamer–target complexes is defined by the dissociation constant, K d , which can extend from the micromolar domain (low affinity) to the picomolar domain (high affinity) [ 41 , 50 ].…”

Section: The Role Of Aptamers In Developing Biosensors For Food Contaminantsmentioning

confidence: 99%

“…Generally, these strategies exploit differences in the flexibility of ssDNA and double-stranded DNA (dsDNA) structures, along with their transition towards secondary structures such as stem and loop structures, and are known as electrochemical molecular beacons [ 46 , 103 ], in analogy with previously developed fluorescent molecular beacons [ 104 ]. These approaches have been reviewed in several papers that highlight possible detection mechanisms for electrochemical aptasensors [ 41 , 42 , 45 , 50 , 90 , 105 , 106 , 107 ]. The strategies adopting covalently labeled aptamers are commonly classified as: assays based on target binding-induced conformational change of the aptamers, assays based on target binding-induced aptamer-complementary strand displacement, and sandwich assays [ 50 , 90 ].…”

Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

“…These approaches have been reviewed in several papers that highlight possible detection mechanisms for electrochemical aptasensors [ 41 , 42 , 45 , 50 , 90 , 105 , 106 , 107 ]. The strategies adopting covalently labeled aptamers are commonly classified as: assays based on target binding-induced conformational change of the aptamers, assays based on target binding-induced aptamer-complementary strand displacement, and sandwich assays [ 50 , 90 ]. For the label-free approaches, the target–aptamer interaction is detected using redox molecules that interact diffusively or associate electrostatically with the surface-bound aptamers [ 90 , 106 ].…”

Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

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The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

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Food safety monitoring assays based on synthetic recognition structures such as aptamers are receiving considerable attention due to their remarkable advantages in terms of their ability to bind to a wide range of target analytes, strong binding affinity, facile manufacturing, and cost-effectiveness. Although aptasensors for food monitoring are still in the development stage, the use of an electrochemical detection route, combined with the wide range of materials available as transducers and the proper immobilization strategy of the aptamer at the transducer surface, can lead to powerful analytical tools. In such a context, employing aryldiazonium salts for the surface derivatization of transducer electrodes serves as a simple, versatile and robust strategy to fine-tune the interface properties and to facilitate the convenient anchoring and stability of the aptamer. By summarizing the most important results disclosed in the last years, this article provides a comprehensive review that emphasizes the contribution of aryldiazonium chemistry in developing electrochemical aptasensors for food safety monitoring.

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“…Among the various DDS reported, nucleic acids-based, artificial structures catch researchers' eyes since they are highly biocompatible, conveniently prepared, multi-functional, and thus exhibit great potential in DDS. 1,2 Especially, aptamers are essentially short RNA or single-stranded DNA oligonucleotides (usually 20-80 nucleotides with 6-30 kDa molecular weights) that can fold into unique three-dimensional conformations. In recent decades, aptamers have played critical roles in DDS because of their unique characteristics such as programmability, flexibility, and low toxicity.…”

Section: Introductionmentioning

confidence: 99%

VEGF aptamer/i-motif-grafted multi-functional SPION nanocarrier for chemotherapeutic/phototherapeutic synergistic research

Fu¹,

Lin

Liu³

et al. 2021

J Biomater Appl

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Chemotherapeutic agents and photosensitizers often suffer from poor tumor selectivity, high side toxicity, or low water solubility. To address these problems, various drug delivery systems (DDS) have been explored but most of them are toxic, difficult to synthesize, or of single function. In order to design a highly biocompatible, conveniently prepared, multi-functional drug delivery system, herein, an aptamer of vascular endothelial growth factor (VEGF) and a cytosine (C)-DNA fragment were grafted on the surface of superparamagnetic iron oxide nanoparticles (SPION), and then a chemotherapeutic agent daunomycin (DNM) and a photosensitizer 5, 10, 15, 20-tetra (phenyl-4-N-methyl-4-pyridyl) porphyrin (TMPyP) were self-assembled with the hybridized VEGF-based DNA structure. By loading DNM and TMPyP, the DDS displayed strong chemotherapeutic/phototherapeutic capability against cancer cells via mechanisms such as mitochondrial dysfunction and ROS elevation, which triggered the apoptosis of the tumor cells. The dual delivery of chemotherapeutical agents and photosensitizers with aptamer/C-rich DNA successfully integrated the functions of pH stimuli-responsive drug release and chemotherapeutic/phototherapeutic modalities into one single system and thus could be considered as an ideal drug delivery vehicle with great potential in clinic.

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Aptamers in biomedicine: Selection strategies and recent advances

Cited by 75 publications

References 244 publications

A Review of Detection of Antibiotic Residues in Food by Surface-Enhanced Raman Spectroscopy

A Review of Detection of Antibiotic Residues in Food by Surface-Enhanced Raman Spectroscopy

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

VEGF aptamer/i-motif-grafted multi-functional SPION nanocarrier for chemotherapeutic/phototherapeutic synergistic research

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