Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

Ramanathan, Santheraleka; Ismail, Zool Hilmi; Arshad, M.K. Md; Poopalan, P.

doi:10.1016/j.bios.2021.113735

Cited by 69 publications

(33 citation statements)

References 42 publications

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“…The aptasensor was also checked for its stability and reusability and showed ~30–33% of activity loss after 11 days of analysis. Not only that, the interaction between NCP aptamer and protein was also confirmed through ELISA [ 118 ].…”

Section: Label and Label-free Aptasensorsmentioning

confidence: 96%

“… Illustration of electrochemical aptasensors for the detection of SARS-CoV-2. ( A ) Schematic of nucleocapsid protein detection of SARS-CoV-2 through immobilization of anti-NCP aptamer on diamond-enhanced gold interdigitated electrode (reprinted with permission from [ 118 ]. Copyright 2021 Elsevier) ( B ) Representation of dual-aptamer-based biosensor for nucleocapsid protein detection of SARS-CoV-2 by utilizing metal organic framework decorated with Au@Pt nanoparticle (reprinted with permission from [ 88 ].…”

Section: Figurementioning

confidence: 99%

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Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

et al. 2022

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Viral infections are becoming the foremost driver of morbidity, mortality and economic loss all around the world. Treatment for diseases associated to some deadly viruses are challenging tasks, due to lack of infrastructure, finance and availability of rapid, accurate and easy-to-use detection methods or devices. The emergence of biosensors has proven to be a success in the field of diagnosis to overcome the challenges associated with traditional methods. Furthermore, the incorporation of aptamers as bio-recognition elements in the design of biosensors has paved a way towards rapid, cost-effective, and specific detection devices which are insensitive to changes in the environment. In the last decade, aptamers have emerged to be suitable and efficient biorecognition elements for the detection of different kinds of analytes, such as metal ions, small and macro molecules, and even cells. The signal generation in the detection process depends on different parameters; one such parameter is whether the labelled molecule is incorporated or not for monitoring the sensing process. Based on the labelling, biosensors are classified as label or label-free; both have their significant advantages and disadvantages. Here, we have primarily reviewed the advantages for using aptamers in the transduction system of sensing devices. Furthermore, the labelled and label-free opto-electrochemical aptasensors for the detection of various kinds of viruses have been discussed. Moreover, numerous globally developed aptasensors for the sensing of different types of viruses have been illustrated and explained in tabulated form.

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Section: Label and Label-free Aptasensorsmentioning

confidence: 96%

Section: Figurementioning

confidence: 99%

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

et al. 2022

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“…Nanomaterials serve as labels that allow for significant signal improvements. Ramanathan et al [ 67 ] deposited ~ 20 nm diamond on the surface of a 10-μm notched gold interdigitated electrode (AuIDE), which enhanced the sensing of SARS-CoV-2 N protein within an LOD of 0.389 fM (Fig. 5A ).…”

Section: Advances In Functional Nucleic Acid-based Technology To Over...mentioning

confidence: 99%

“…5 A Schematic of N protein detection using an anti-N protein aptamer on nanodiamond-enhanced AuIDE. Reproduced with permission from [ 67 ] , Copyright 2022, Elsevier . B Schematic of the Nb2C-SH QD-based SPR aptasensor for detecting the N gene of SARS-CoV-2.…”

Section: Advances In Functional Nucleic Acid-based Technology To Over...mentioning

confidence: 99%

Recent advances of functional nucleic acid-based sensors for point-of-care detection of SARS-CoV-2

Zhang

Feng

et al. 2022

Microchim Acta

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This review focuses on critical scientific barriers that the field of point-of-care (POC) testing of SARS-CoV-2 is facing and possible solutions to overcome these barriers using functional nucleic acid (FNA)-based technology. Beyond the summary of recent advances in FNA-based sensors for COVID-19 diagnostics, our goal is to outline how FNA might serve to overcome the scientific barriers that currently available diagnostic approaches are suffering. The first introductory section on the operationalization of the COVID-19 pandemic in historical view and its clinical features contextualizes essential SARS-CoV-2-specific biomarkers. The second part highlights three major scientific barriers for POC COVID-19 diagnosis, that is, the lack of a general method for (1) designing receptors of SARS-CoV-2 variants; (2) improving sensitivity to overcome false negatives; and (3) signal readout in resource-limited settings. The subsequent part provides fundamental insights into FNA and technical tricks to successfully achieve effective COVID-19 diagnosis by using in vitro selection of FNA to overcome receptor design barriers, combining FNA with multiple DNA signal amplification strategies to improve sensitivity, and interfacing FNA with portable analyzers to overcome signal readout barriers. This review concludes with an overview of further opportunities and emerging applications for FNA-based sensors against COVID-19. Graphical abstract

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“…However, the price of a single test and the time it takes have forced the entire scientific community to research and develop new ways to quickly and cheaply detect SARS-Cov-2. Recently, impedimetric (Abrego-Martinez et al, 2022;Büyüksünetçi et al, 2022;Kumar et al, 2022;Ramanathan et al, 2022) and resistive (Mattioli et al, 2022;Torrente-Rodríguez et al, 2020) biosensors were considered as selective and rapid platforms for the determination of the SARS-CoV-2 spike S1 protein. Despite their advantages (high sensitivity or selectivity), these methods have important limitations.…”

Section: Introductionmentioning

confidence: 99%

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: multivariate data analysis of multisine impedimetric response

Brodowski

Pierpaoli

Janik

et al. 2022

Preprint

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of spike protein to the host cell surface-expressing angiotensin-converting enzyme 2 (ACE2) or by endocytosis mediated by extracellular matrix metalloproteinase inducer (CD147). We present extended statistical studies of the multisine dynamic electrochemical impedance spectroscopy (DEIS) revealing interactions between Spike RBD and cellular receptors ACE2 and CD147, and a reference anti-RBD antibody (IgG2B) based on a functionalised boron-doped diamond (BDD) electrode. The DEIS was supported by a multivariate data analysis of a SARS-CoV-2 Spike RBD assay and cross-correlated with the atomic-level information revealed by molecular dynamics simulations. Our approach allowed us to study and detect subtle changes in the electrical properties responsible for the susceptibility of cellular receptors to SARS-CoV-2, revealing their interactions. Changes in electrical homogeneity in the function of the RBD concentration led to the conclusion that the ACE2 receptor delivers the most homogeneous surface, delivered by the high electrostatic potential of the relevant docking regions. For higher RBD concentrations, the differences in electrical homogeneity between electrodes with different receptors vanish. Collectively, this study reveals interdependent virus entry paths involving separately ACE2, CD147, and spike protein, provided by a developed biosensing platform for the rapid screening of cellular interactions (i.e. testing various mutations of SARS-CoV-2 or screening of therapeutic drugs).

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Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

Cited by 69 publications

References 42 publications

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

Recent advances of functional nucleic acid-based sensors for point-of-care detection of SARS-CoV-2

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: multivariate data analysis of multisine impedimetric response

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