Aptasensors for Staphylococcus aureus Risk Assessment in Food

Huang, Ziqian; Yu, Xin; Yang, Qingli; Zhao, Ying; Wu, Wei

doi:10.3389/fmicb.2021.714265

Cited by 20 publications

(12 citation statements)

References 81 publications

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“…The demand for deployable tests beyond clinical diagnostics is emerging, as evidenced by research in areas such as water quality, aquaculture, and environmental monitoring [ 79 , 80 , 81 ], food and beverage quality and authentication [ 82 , 83 , 84 ], detection of food allergens [ 85 ], and detection of pyrogens such as endotoxin in biotechnology products [ 86 ]. In conclusion, as a paradigm of the inventive use of plasmonic biosensors, a recent niche application is worth mentioning: the rapid determination of whisky age by SPR [ 87 ].…”

Section: Discussion Conclusion and Future Directionsmentioning

confidence: 99%

Gold Nanoparticle-Based Plasmonic Biosensors

Ferrari

2023

Biosensors

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One of the emerging technologies in molecular diagnostics of the last two decades is the use of gold nanoparticles (AuNPs) for biosensors. AuNPs can be functionalized with various biomolecules, such as nucleic acids or antibodies, to recognize and bind to specific targets. AuNPs present unique optical properties, such as their distinctive plasmonic band, which confers a bright-red color to AuNP solutions, and their extremely high extinction coefficient, which makes AuNPs detectable by the naked eye even at low concentrations. Ingenious molecular mechanisms triggered by the presence of a target analyte can change the colloidal status of AuNPs from dispersed to aggregated, with a subsequent visible change in color of the solution due to the loss of the characteristic plasmonic band. This review describes how the optical properties of AuNPs have been exploited for the design of plasmonic biosensors that only require the simple mixing of reagents combined with a visual readout and focuses on the molecular mechanisms involved. This review illustrates selected examples of AuNP-based plasmonic biosensors and promising approaches for the point-of-care testing of various analytes, spanning from the viral RNA of SARS-CoV-2 to the molecules that give distinctive flavor and color to aged whisky.

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Section: Discussion Conclusion and Future Directionsmentioning

confidence: 99%

Gold Nanoparticle-Based Plasmonic Biosensors

Ferrari

2023

Biosensors

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“…Biosensors based on aptamers are referred to as aptasensors. Given their high structural stability and capability of recovering from denaturation, aptasensors are able to detect a variety of targets such as metal ions, small molecules, proteins, pathogens, and other nucleic acids [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. The main advantage of aptamers over other biorecognition molecules such as antibodies and enzymes is that they can be developed using combinatorial generation methods, such as Systematic Evolution of Ligands by Exponential Enrichment (SELEX), rather than requiring cultured organisms or animal hosts [ 20 ].…”

Section: Detection Methodsmentioning

confidence: 99%

Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens

et al. 2022

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Paper-based biosensors are microfluidic analytical devices used for the detection of biochemical substances. The unique properties of paper-based biosensors, including low cost, portability, disposability, and ease of use, make them an excellent tool for point-of-care testing. Among all analyte detection methods, nucleic acid-based pathogen detection offers versatility due to the ease of nucleic acid synthesis. In a point-of-care testing context, the combination of nucleic acid detection and a paper-based platform allows for accurate detection. This review offers an overview of contemporary paper-based biosensors for detecting nucleic acids from pathogens. The methods and limitations of implementing an integrated portable paper-based platform are discussed. The review concludes with potential directions for future research in the development of paper-based biosensors.

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“…aureus. Conventional culture methods, although considered to be gold standards for the detection of bacteria, are reliable and accurate, but they are considerably time-consuming (typically 2–3 days). − More recent approaches involve nucleic acid amplification techniques such as the polymerase chain reaction (PCR) for rapid detection of this bacteria. , Although simple to use and widely available, the amplification step can also amplify false targets, and thus, the PCR-based techniques are not free from false positives. , The enzyme-linked immunosorbent assay (ELISA) based on the antigen and antibody interaction is another commonly used method for the detection of bacterial foodborne pathogens due to their high specificity. , However, antibodies are relatively more expensive and have shorter shelf lives than aptamers. , More recently, techniques such as colorimetric, , surface-enhanced Raman scattering (SERS), , and electrochemical methods , have been developed as novel strategies for the detection of S. aureus but they are also not free from limitations.…”

Section: Introductionmentioning

confidence: 99%

“…aureus are based on detecting enterotoxins of S. aureus. ,, The enterotoxin detection showed a wide range of sensitivities from 1.0 pM to 0.05 fM. − However, there are only a few methods explored around using an S. aureus-specific markerIsdAin the detection of this pathogen.…”

Section: Introductionmentioning

confidence: 99%

“…Along this line, recently developed bio-affinity probes such as nucleic acid aptamers would be ideal for high specificity detection of proteins. Compared to antibodies, aptamers can be easily modified and can bind to a single protein or non-protein targets as well as to cells, bacteria, and viruses, making them a great tool in the detection of foodborne pathogens. , Aptamers are generally identified and selected from large libraries of randomly created oligonucleotides using an experimental method called Systematic Evolution of Ligands by Exponential Enrichment (SELEX) . In comparison to experimental SELEX, the computational SELEX uses free energy calculations and binding energy between complexes to design high-affinity aptamers, which could provide the means for more efficient screening of aptamers …”

Section: Introductionmentioning

confidence: 99%

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FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected Aptamers

Wijesinghe,

Sabbih,

Algama

et al. 2023

Anal. Chem.

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Iron-regulated surface determinant protein A (IsdA) is a key surface protein found in the foodborne bacteriaStaphylococcus aureus (S. aureus)which is known to be critical for bacterial survival and colonization. S. aureus is pathogenic and has been linked to foodborne diseases; thus, early detection is critical to prevent diseases caused by this bacterium. Despite IsdA being a specific marker for S. aureus and several detection methods have been developed for sensitive detection of this bacteria such as cell culture, nucleic acids amplification, and other colorimetric and electrochemical methods, the detection of S. aureus through IsdA is underdeveloped. Here, by combining computational generation of target-guided aptamers and fluorescence resonance energy transfer (FRET)-based single-molecule analysis, we presented a widely applicable and robust detection method for IsdA. Three different RNA aptamers specific to the IsdA protein were identified and their ability to switch a FRET construct to a high-FRET state in the presence of protein was verified. The presented approach demonstrated the detection of IsdA down to picomolar levels (×10–12 M, equivalent to ∼1.1 femtomoles IsdA) with a dynamic range extending to ∼40 nM. The FRET-based single-molecule technique that we reported here is capable of detecting the foodborne pathogen protein IsdA with high sensitivity and specificity and has a broader application in the food industry and aptamer-based sensing field by enabling quantitative detection of a wide range of pathogen proteins.

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Aptasensors for Staphylococcus aureus Risk Assessment in Food

Cited by 20 publications

References 81 publications

Gold Nanoparticle-Based Plasmonic Biosensors

Gold Nanoparticle-Based Plasmonic Biosensors

Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens

FRET-Based Single-Molecule Detection of Pathogen Protein IsdA Using Computationally Selected Aptamers

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