Aptasensors for the detection of infectious pathogens: design strategies and point-of-care testing

Chen, Xiaofei; Zhao, Xin; Yang, Zifeng

doi:10.1007/s00604-022-05533-w

Cited by 23 publications

(15 citation statements)

References 230 publications

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“…MNPs with proper surface functionalization are especially suitable for the development of lab-in-chip biosensors for easy, reliable, and cost-effective POC diagnostic facilities. In addition, MNPs are widely used in the molecular diagnosis of human viruses [ 128 ] and other infectious pathogens [ 129 ]; MNP-based biosensors are also an extremely promising form in highly accurate POC SARS-CoV-2 detection [ 130 , 131 , 132 ]. In this review, we briefly highlight the following biological objects, which have been recently analyzed using MNP-based POC devices: SARS-CoV-2, Human chorionic gonadotropin (hCG), the typical oral pathogen of Streptococcus, foodborne bacteria in food and environmental samples ( Listeria monocytogenes , Campylobacter jejuni , and Staphylococcus aureus ), pathogenic bacteria ( Escherichia coli ( E. coli ), and Staphylococcus aureus ( S. aureus ), the food pathogen Vibrio parahaemolyticus ), the bacteria Acinetobacter baumannii , and influenza A H1N1 virus ( Table 1 ).…”

Section: Diagnostic Application Of Mnpsmentioning

confidence: 99%

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application

et al. 2023

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In the last few decades, point-of-care (POC) sensors have become increasingly important in the detection of various targets for the early diagnostics and treatment of diseases. Diverse nanomaterials are used as building blocks for the development of smart biosensors and magnetite nanoparticles (MNPs) are among them. The intrinsic properties of MNPs, such as their large surface area, chemical stability, ease of functionalization, high saturation magnetization, and more, mean they have great potential for use in biosensors. Moreover, the unique characteristics of MNPs, such as their response to external magnetic fields, allow them to be easily manipulated (concentrated and redispersed) in fluidic media. As they are functionalized with biomolecules, MNPs bear high sensitivity and selectivity towards the detection of target biomolecules, which means they are advantageous in biosensor development and lead to a more sensitive, rapid, and accurate identification and quantification of target analytes. Due to the abovementioned properties of functionalized MNPs and their unique magnetic characteristics, they could be employed in the creation of new POC devices, molecular logic gates, and new biomolecular-based biocomputing interfaces, which would build on new ideas and principles. The current review outlines the synthesis, surface coverage, and functionalization of MNPs, as well as recent advancements in magnetite-based biosensors for POC diagnostics and some perspectives in molecular logic, and it also contains some of our own results regarding the topic, which include synthetic MNPs, their application for sample preparation, and the design of fluorescent-based molecular logic gates.

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Section: Diagnostic Application Of Mnpsmentioning

confidence: 99%

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application

et al. 2023

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“…By using this method, specific interactions between the target bacterium and the recognition molecule on the QCM chip surface could be created for the quick determination of foodborne pathogens in food samples. Although many methods have been reported to detect pathogens in food samples, nonspecific interactions hinder real-life applications of sensor systems [ 19 , 25 – 27 ]. Therefore, the magnetic pre-enrichment technique has been used to prevent the interaction of impurities with the QCM system for the isolation of protein, cells, and fine chemicals from complex solutions without using expensive laboratory equipment.…”

Section: Introductionmentioning

confidence: 99%

Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

Beyazit,

Arica,

Acikgoz-Erkaya

et al. 2024

Microchim Acta

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A fast and accurate identification of Listeria monocytogenes. A new quartz crystal microbalance (QCM) aptasensor was designed for the specific and rapid detection of L. monocytogenes. Before detection of the target bacterium from samples in the QCM aptasensor, a magnetic pre-enrichment system was used to eliminate any contaminant in the samples. The prepared magnetic system was characterized using ATR-FTIR, SEM, VSM, BET, and analytical methods. The saturation magnetization values of the Fe3O4, Fe3O4@PDA, and Fe3O4@PDA@DAPEG particles were 57.2, 40.8, and 36.4 emu/g, respectively. The same aptamer was also immobilized on the QCM crystal integrated into QCM flow cell and utilized to quantitatively detect L. monocytogenes cells from the samples. It was found that a specific aptamer-magnetic pre-concentration system efficiently captured L. monocytogenes cells in a short time (approximately 10 min). The Fe3O4@PDA@DA-PEG-Apt particles provided selective isolation of L. monocytogenes from the bacteria-spiked media up to 91.8%. The immobilized aptamer content of the magnetic particles was 5834 µg/g using 500 ng Apt/mL. The QCM aptasensor showed a very high range of analytical performance to the target bacterium from 1.0 × 102 and 1.0 × 107 CFU/mL. The limit of detection (LOD) and limit of quantitation (LOQ) were 148 and 448 CFU/mL, respectively, from the feeding of the QCM aptasensor flow cell with the eluent of the magnetic pre-concentration system. The reproducibility of the aptasensor was more than 95%. The aptasensor was very specific to L. monocytogenes compared to the other Listeria species (i.e., L. ivanovii, L. innocua, and L. seeligeri) or other tested bacteria such as Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. The QCM aptasensor was regenerated with NaOH solution, and the system was reused many times. Graphical Abstract

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“…From the point of view of medical diagnostics, assay accuracy as well as sensitivity and specificity (defined in biomedical terms) play a key role. The capability of the determination of trace concentrations of analytes, which enable the detection of various disease markers (e.g., the presence of viruses or bacteria) as well other abnormalities of the human body (usually manifested by the elevated levels of specific biomarkers), harmful chemical contamination in the environment, or poor-quality food, is also of great importance in various areas of life [ 2 , 3 , 4 , 5 , 6 ]. An important branch of modern analytical devices in this area is chemical sensors, which, still strive for the lack of sufficient sensitivity and selectivity in the case of the analysis of complex samples.…”

Section: Introductionmentioning

confidence: 99%

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Szymczyk

Ziółkowski

Malinowska

2023

Sensors

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To meet the requirements of novel therapies, effective treatments should be supported by diagnostic tools characterized by appropriate analytical and working parameters. These are, in particular, fast and reliable responses that are proportional to analyte concentration, with low detection limits, high selectivity, cost-efficient construction, and portability, allowing for the development of point-of-care devices. Biosensors using nucleic acids as receptors has turned out to be an effective approach for meeting the abovementioned requirements. Careful design of the receptor layers will allow them to obtain DNA biosensors that are dedicated to almost any analyte, including ions, low and high molecular weight compounds, nucleic acids, proteins, and even whole cells. The impulse for the application of carbon nanomaterials in electrochemical DNA biosensors is rooted in the possibility to further influence their analytical parameters and adjust them to the chosen analysis. Such nanomaterials enable the lowering of the detection limit, the extension of the biosensor linear response, or the increase in selectivity. This is possible thanks to their high conductivity, large surface-to-area ratio, ease of chemical modification, and introduction of other nanomaterials, such as nanoparticles, into the carbon structures. This review discusses the recent advances on the design and application of carbon nanomaterials in electrochemical DNA biosensors that are dedicated especially to modern medical diagnostics.

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Aptasensors for the detection of infectious pathogens: design strategies and point-of-care testing

Cited by 23 publications

References 230 publications

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application

Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

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