APTES mediated modular modification of regenerated silk fibroin in a water solution

Sagnella, Anna; Zambianchi, Massimo; Durso, Margherita; Posati, Tamara; Río, Alberto Del; Donnadio, Anna; Mazzanti, Andrea; Pistone, Assunta; Ruani, G.; Zamboni, R.; Benfenati, Valentina; Melucci, Manuela

doi:10.1039/c5ra10016j

Cited by 13 publications

(8 citation statements)

References 29 publications

(39 reference statements)

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“…In this view, analyses and comparison of samples obtained by blending and addition to SF solution of the selected compound with samples obtained by doping diet method revealed that the sample obtained by doping diet method displays a similar efficiency of functionalization of the substrates. Moreover the method does not affect SF structural, chemophysical and biological property [14,24,27,28].…”

Section: Conclusion and Future Researchmentioning

confidence: 99%

Silk Fibroin Based Technology for Industrial Biomanufacturing

Benfenati

Toffanin

Chieco

et al. 2019

Factories of the Future

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Natural biomaterials are more and more used for the development of high technology solutions, setting the scene for a bio-based material economy that responds to the increasing demand of environmentally friendly products. Among natural biomaterials, silk fibre protein called silk fibroin (SF) produced by the Bombyx mori L. insect, recently found a broad range of applications in biomedical field. SF substrates display remarkable properties like controlled biodegradability, flexibility, mechanical resistance and optical transparency, solution processability. These properties combined with the water-based extraction and purification process make SF a promising material for sustainable manufacturing enabling to partially replace synthetic, plastic-based and non-biodegradable material use. The use of SF interfaces in biocompatible electronic or photonic devices for advanced biomedical applications has been recently highlighted. However, the use of a natural biomaterial is challenging due to the complex nature of the biological molecule, and it requires to tightly control biomaterial properties during all the manufacturing steps. In this work, we show the results obtained by in loco production of raw-material, defining the best condition for silkworm selection and growth. The assessment and standardization of extraction/purification methodology are reported with reference to the high purity and remarkable performance in terms of chemo-physical property and biocompatibility of the obtained SF products. Finally, we demonstrate the fabrication, characterization and validation of microfluidic and photonic components of a lab-on-a-chip device for biodiagnostic based on biomanufactured SF.

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Section: Conclusion and Future Researchmentioning

confidence: 99%

Silk Fibroin Based Technology for Industrial Biomanufacturing

Benfenati

Toffanin

Chieco

et al. 2019

Factories of the Future

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“…In recent years, silk fibroin has been eliciting ever-increasing interest in the biotechnology field. Several applications have been reported, spanning tissue engineering , to regenerative medicine, − drug delivery − to biophotonics, − and implantable devices − to the last frontiers in bioengineering and the realization of biodegradable, bioresorbable, and edible electronic devices. − The main reason behind such a success story is the peculiar advantages offered by silk, in terms of outstanding mechanical properties, chemical–physical versatility due to its polymorphic character, thermal and environmental stability, suitability to several processing techniques (easily allowing for covering dimensions from the nano- to the macroscale), endless opportunities of functionalization with biomolecules and drugs, and excellent cytocompatibility. Overall, silk is widely recognized as a highly promising material platform, with global impact in the biomedical, biophotonics, and bioelectronics fields …”

Section: Introductionmentioning

confidence: 99%

Micro- and Nanopatterned Silk Substrates for Antifouling Applications

Tullii

Donini

Bossio

et al. 2020

ACS Appl. Mater. Interfaces

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A major problem of current biomedical implants is the bacterial colonization and subsequent biofilm formation, which seriously affects their functioning and can lead to serious post-surgical complications. Intensive efforts have been directed toward the development of novel technologies that can prevent bacterial colonization while requiring minimal antibiotics doses. To this end, biocompatible materials with intrinsic antifouling capabilities are in high demand. Silk fibroin, widely employed in biotechnology, represents an interesting candidate.Here, we employ a soft-lithography approach to realize micro-and nanostructured silk fibroin substrates, with different geometries. We show that patterned silk film substrates support mammal cells (HEK-293) adhesion and proliferation, and at the same time, they intrinsically display remarkable antifouling properties. We employ Escherichia coli as representative Gram-negative bacteria, and we observe an up to 66% decrease in the number of bacteria that adhere to patterned silk surfaces as compared to control, flat silk samples. The mechanism leading to the inhibition of biofilm formation critically depends on the microstructure geometry, involving both a steric and a hydrophobic effect. We also couple silk fibroin patterned films to a biocompatible, optically responsive organic semiconductor, and we verify that the antifouling properties are very well preserved. The technology described here is of interest for the next generation of biomedical implants, involving the use of materials with enhanced antibacterial capability, easy processability, high biocompatibility, and prompt availability for coupling with photoimaging and photodetection techniques.

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“…The repetitive GAGAGX hexapeptide units (X = S, Y, or A), which form an antiparallel β-sheet structure, are the main component of SF and are essential for exhibiting high tensile strengths. − Several attempts to modify the properties of SF and to add novel functions have been reported. For example, coupling reactions using cyanuric chloride, − carbodiimide, − glutaraldehyde, alkoxysilane, and isocyanate have been demonstrated, whereas the diazonium coupling chemistry was also applied in terms of the selective introduction of unnatural functional groups to the tyrosine residues. , Enzymatic modification is also an attractive approach because the highly selective modification of complicated compounds can be achieved under mild conditions. − The introduction of poly(2,6-dimethylenephenylene ether) onto the side chain of SF via the grafting-from method using horseradish peroxidase as a catalyst has been developed in our group to impart hydrophobicity to silk materials, while Freddi and co-workers reported the tyrosinase-catalyzed modification of SF to graft the polysaccharide chitosan. , In addition, the fabrication of silk composites with other materials such as polymers − and inorganics provided the desired properties. , The development of these approaches is significantly important because of the high potential for the practical use of silk-based materials.…”

Section: Introductionmentioning

confidence: 99%

3,4-Dihydroxyphenylalanine (DOPA)-Containing Silk Fibroin: Its Enzymatic Synthesis and Adhesion Properties

Sogawa

Ifuku

Numata

2019

ACS Biomater. Sci. Eng.

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Silk fibroin (SF) is a fascinating natural biomaterial that exhibits remarkable mechanical properties and biocompatibility. Meanwhile, biological adhesive materials have gathered much attention as biomedical and ecofriendly materials as a result of their characteristic properties. Herein, we report the excellent adhesive function of enzymatically modified SF. The tyrosine residues of SF were successfully converted to the dihydroxy-l-phenylalanine (DOPA) unit using tyrosinase as a biocatalyst. The content of DOPA was evaluated by amino acid composition analysis. Adhesive functions of DOPA-modified SF (DOPA-SF) among several material surfaces including mica, paper, polypropylene, wood, and silk film were elucidated by lap shear tests. Fourier transform infrared measurements demonstrated that the adhesion strength of DOPA-SF was not directly related to the β-sheet formation of silk molecules. This ecofriendly and facile method offers a new perspective for fabricating natural adhesive materials for various application areas.

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APTES mediated modular modification of regenerated silk fibroin in a water solution

Cited by 13 publications

References 29 publications

Silk Fibroin Based Technology for Industrial Biomanufacturing

Silk Fibroin Based Technology for Industrial Biomanufacturing

Micro- and Nanopatterned Silk Substrates for Antifouling Applications

3,4-Dihydroxyphenylalanine (DOPA)-Containing Silk Fibroin: Its Enzymatic Synthesis and Adhesion Properties

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