Aptness of Escherichia coli host strain CB390 to detect total coliphages in Colombia

Abstract

Fecal bacteria have been used for more than a century as indicators of fecal contamination in water. In recent years, the monitoring of somatic and F-specific coliphages has been gradually included in guidelines and regulations as an additional parameter to reinforce water safety. The Escherichia coli host strain CB390 was tailored to detect both somatic and F-specific coliphages in a single test. The efficacy of this strain for bacteriophage detection, previously evaluated in Western Europe and North America, was assessed here for the first time in South America. The detection of somatic and F-specific coliphages by the strain CB390, as well as by standardized methods, was performed in drinking and river water and municipal and abattoir wastewaters. No statistical difference was found in the numbers of total coliphages detected by strain CB390 and the sum of somatic and F-specific coliphages determined separately by the standardized ISO methods. The data presented here provide further validation of the effectiveness of the host strain E. coli CB390 for the detection of total coliphages in waters in a single test and demonstrate its suitability for application in upper-middle income countries of the Americas (World Bank category).

Helicobacter pylori virulence genotypes in Bogotá River and wastewater treatment plants in Colombia

“…However, raw water and water from the rivers are used as a source for human consumption, reusing and reincorporating it into different ecosystems despite its low quality 2–5 . In Colombia, domestic wastewater treatment rates have increased in recent years 6,7 ; although in some areas of the country there are still low rates of coverage for the treatment and thus, discharge of low‐quality wastewater is occurring, mainly in terms of physicochemical and microbiological conditions, leading to the contamination of the rivers without control of governmental parties becoming an important public health affair 8–10 …”

“…Colombia, domestic wastewater treatment rates have increased in recent years 6,7 ; although in some areas of the country there are still low rates of coverage for the treatment and thus, discharge of lowquality wastewater is occurring, mainly in terms of physicochemical and microbiological conditions, leading to the contamination of the rivers without control of governmental parties becoming an important public health affair. [8][9][10] In Colombia, according to Resolution 2115 of 2007, 11 water disposed for human consumption should be free of fecal microorganisms including total coliforms, Escherichia coli (E. coli), heterotrophs, Cryptosporidium sp. oocysts, and Giardia sp.…”

Evaluación de la actividad antimicrobiana de extractos del género Passiflora y búsqueda de bacteriófagos con actividad lítica frente a Helicobacter pylori

“…Cepa aportada por el Laboratorio de Indicadores de Calidad de Aguas y Lodos de la Universidad Javeriana [210].…”

“…Así mismo, se emplearon controles de esterilidad del medio y de los filtrados, control de crecimiento bacteriano y un control de calidad de los procedimientos, para el cual se realizó un ensayo de inhibición con la cepa WG5 de E. coli y un colifago aportado por el Laboratorio de Indicadores de Calidad de Aguas y Lodos de la Universidad Javeriana [210].…”