aPTT: 1st Recognition for Human Whole Blood on QCM-D Platform

Hussain, Munawar

doi:10.20510/ukjpb/3/i6/87835

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…The resonant frequency of the BAW transducer is sensitive to the small mass addition on the upper surface or the change in the viscosity of adjacent mediums. QCM devices have been successfully used to monitor the blood coagulation in real time [ 9 ] and measure several hemostatic parameters, including activated partial thromboplastin time (aPTT) [ 10 ], prothrombinase induced clotting time (PiCT) [ 11 ], thrombin times (TTs) [ 12 ], prothrombin time (PT) [ 13 ] and fibrinogen [ 14 ]. This technology shows an outstanding application prospect in the point-of-care (POC) system.…”

Section: Introductionmentioning

confidence: 99%

ZnO Film Bulk Acoustic Resonator for the Kinetics Study of Human Blood Coagulation

Chen

Zhang

et al. 2017

Sensors

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Miniaturized and rapid blood coagulation assay technologies are critical in many clinical settings. In this paper, we present a ZnO film bulk acoustic resonator for the kinetic analysis of human blood coagulation. The resonator operated in thickness shear resonance mode at 1.4 GHz. When the resonator contacted the liquid environment, the viscous loading effect was considered as the additional resistance and inductance in the equivalent circuits, resulting in a linear relationship with a slope of approximately −217 kHz/cP between the liquid viscosity and the frequency of the resonator. The downshift of the resonant frequency and the viscosity change during the blood coagulation were correlated to monitor the coagulation process. The sigmoidal trend was observed in the frequency response for the blood samples activated by thromboplastin and calcium ions. The coagulation kinetics involving sequential phases of steady reaction, growth and saturation were revealed through the time-dependent frequency profiles. The enzymatic cascade time, the coagulation rate, the coagulation time and the clot degree were provided by fitting the time-frequency curves. The prothrombin times were compared with the results measured by a standard coagulometer and show a good correlation. Thanks to the excellent potential of integration, miniaturization and the availability of direct digital signals, the film bulk acoustic resonator has promising application for both clinical and personal use coagulation testing technologies.

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Section: Introductionmentioning

confidence: 99%

ZnO Film Bulk Acoustic Resonator for the Kinetics Study of Human Blood Coagulation

Chen

Zhang

et al. 2017

Sensors

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“…Biological approaches consider rising antibodies allowing topological recognitions of different HS/heparin domains, although the specificity of particular sequence recognition at the structural level is still far to be reached at sufficient levels . Among functional approaches, heparin—the most used anticoagulant in surgery—is controlled via anticoagulant standard tests such as thromboplastin time and activated partial thromboplastin time for proper therapeutic outcomes . Thus, although important advances keep ongoing, development of new approaches capable to detect specific HS/heparin fractions or sequences is still required.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, MIPs have been generated for a wide range of applications, including chromatography, solid‐phase extraction, reaction catalysis, and biomimetic sensors . Particularly, recognition of heparin by molecularly imprinted surfaces has been introduced by Hussain and co‐workers . However, approaches allowing recognition of particular sulfated sequences in HS and/or heparin remain to be developed.…”

Section: Introductionmentioning

confidence: 99%

Poly(ethylene glycol acrylate)‐functionalized hydrogels for heparan sulfate oligosaccharide recognition

Mothéré

Singabraya

Driguez

et al. 2016

J of Molecular Recognition

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Heparan sulfates are complex polysaccharides belonging to the family of glycosaminoglycans that participate to the regulation of cell behavior and tissue homeostasis. The biological activities conferred to heparan sulfates are largely dependent on the content and positioning of the sulfate groups along their saccharidic units. At present, identification of particular sulfation patterns in biologically relevant heparan sulfate sequences remains challenging. Although several approaches for structure analysis exist, the complexity of heparan sulfates makes new and original approaches still required. Here, we used molecular imprinting technologies to prepare a library of polyethylene glycol acrylate functionalized hydrogels with the aim to investigate their applicability as specific recognizing systems for fondaparinux, a synthetic pentasaccharide analog to the antithrombin binding site of heparin. Adequate choice of the hydrogel composition and controlling rebinding conditions were important determinants for improving the sulfated oligosaccharide recognition specificity and selectivity. Our results suggest that molecular imprinting approaches could be a possibility for the specific recognition of biologically active sequences in heparan sulfates.

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“…QCM is well known due to its cost-effectiveness for biosensor applications on comparing to its counterpart sensor techniques 14 . Recently, haemostasis [15][16][17][18][19][20] Technoclone gmbH (Austria).…”

Section: Introductionmentioning

confidence: 99%