Apyrase-mediated amplification of secretory IgA promotes intestinal homeostasis

Perruzza, Lisa; Strati, Francesco; Raneri, Matteo; Li, Hai; Gargari, Giorgio; Rezzonico-Jost, Tanja; Palatella, Martina; Kwee, Ivo; Morone, Diego; Seehusen, Frauke; Sonego, Paolo; Donati, Claudio; Franceschi, Pietro; Guglielmetti, Simone; Greiff, Victor; Grassi, Fabio

doi:10.1016/j.celrep.2022.111112

Cited by 8 publications

(10 citation statements)

References 73 publications

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“…Although IgA levels were elevated in both the ALP-1L and ALP-1 M groups, there were no significant differences in their IgA levels from the control group ( p > 0.05). sIgA is a major noninflammatory effector of the mucosal immune system and plays multiple roles in ensuring the integrity of the intestinal ecosystem and reducing the expression of pro-inflammatory cytokines in the gut . After the administration of ALP-1, sIgA levels in feces were increased by 22.6% in the ALP-1L group ( p > 0.05), and ALP-1 M and ALP-1H groups showed a significant increase of 31.08% ( p < 0.05) and 40.0% ( p < 0.05), respectively, compared with the control group (Figure E).…”

Section: Resultsmentioning

confidence: 99%

“…sIgA is a major noninflammatory effector of the mucosal immune system and plays multiple roles in ensuring the integrity of the intestinal ecosystem and reducing the expression of proinflammatory cytokines in the gut. 20 After the administration of ALP-1, sIgA levels in feces were increased by 22 ALP-1L group (p > 0.05), and ALP-1 M and ALP-1H groups showed a significant increase of 31.08% (p < 0.05) and 40.0% (p < 0.05), respectively, compared with the control group (Figure 2E). The increased sIgA in feces suggested that ALP-1 might improve the immune status by promoting intestinal integrity.…”

Section: Effects Of Alp-1 On Splenic T-lymphocyte Subsetsmentioning

confidence: 91%

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Integrated Mechanism of Immune Response Modulation by Arctium Lappa L. Fructans Based on Microbiome and Metabolomics Technologies

Li,

Qiu,

Bai

et al. 2024

J. Agric. Food Chem.

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Arctium lappa L. is widely consumed for its various biological effects, and polysaccharides are its main functional components. The present study aimed to evaluate the immunoregulatory effects of the main polysaccharides from burdock (ALP-1) and reveal the underlying mechanisms. ALP-1 consisted of fructose and glucose (14.57:1) and had a molecular weight of 2757 Da, with typical characteristics of (1 → 2)-linked linear fructans. Oral intake of ALP-1 significantly increased the number of colonic goblet cells, serum immunoglobulin A and immunoglobulin G levels, and fecal secretory immunoglobulin A content as well as upregulated antioxidant enzymes and increased short chain fatty acid production. In addition, ALP-1 administration regulated pro/antiinflammatory cytokines (i.e., interleukin (IL)-1β, IL-6, tumor necrosis factor-α, interferon-γ, and IL-10), intestinal microbiota structure, and the spatial information on key metabolites. Some gut-microbiota-mediated metabolic processes were also significantly altered. These results indicated that ALP-1 could exert beneficial effects on immune responses and intestinal health in healthy mice.

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Section: Resultsmentioning

confidence: 99%

Section: Effects Of Alp-1 On Splenic T-lymphocyte Subsetsmentioning

confidence: 91%

Integrated Mechanism of Immune Response Modulation by Arctium Lappa L. Fructans Based on Microbiome and Metabolomics Technologies

Li,

Qiu,

Bai

et al. 2024

J. Agric. Food Chem.

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“…All these phenomena were dependent on the enhanced breadth of the SIgA response because the same analyses performed in IgA-deficient mice did not show any difference between mice monocolonized with E. coli pApyr and E. coli pBAD28 . These data unravel the importance of the complex interplay between microbiota and adaptive mucosal immunity in conditioning systemic metabolism and the function of eATP in mediating this reciprocal control via P2X7R [ 60 ]. SIgA amplification by apyrase could be exploited to beneficially affect gut microbial homeostasis during environmental distresses, such as in antibiotics-induced dysbiosis.…”

Section: P2x7r In Controlling Intestinal Secretory Igamentioning

confidence: 99%

The P2X7 receptor in mucosal adaptive immunity

Grassi

Marino

2023

Purinergic Signalling

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The P2X7 receptor (P2X7R) is a widely distributed cation channel activated by extracellular ATP (eATP) with exclusive peculiarities with respect to other P2XRs. In recent years, P2X7R has been shown to regulate the adaptive immune response by conditioning T cell signaling and activation as well as polarization, lineage stability, cell death, and function in tissues. Here we revise experimental observations in this field, with a focus on adaptive immunity at mucosal sites, particularly in the gut, where eATP is hypothesized to act in the reciprocal conditioning of the host immune system and commensal microbiota to promote mutualism. The importance of P2X7R activity in the intestine is consistent with the transcriptional upregulation of P2xr7 gene by retinoic acid, a metabolite playing a key role in mucosal immunity. We emphasize the function of the eATP/P2X7R axis in controlling T follicular helper (Tfh) cell in the gut-associated lymphoid tissue (GALT) and, consequently, T-dependent secretory IgA (SIgA), with a focus on high-affinity SIgA-mediated protection from enteropathogens and shaping of a beneficial microbiota for the host.

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“…They found that hydrolyzed eATP enabled the expansion of the SIgA gene pool. 129 Thus, intestinal health can be restored under abiotic conditions by combining SIgA with ATP hydrolysis. By interacting with the A 2A R, adenosine increased the number of regulatory T cells (Treg) and upregulated their immunosuppressive activity.…”

Section: Induction Of Intestinal Secreted Immunoglobulin a (Siga) By Iapmentioning

confidence: 99%

“…Perruzza et al showed that SIgA improved intestinal homeostasis, attenuated intestinal pathogen damage, and reduced infection susceptibility in ecological dissonance induced by barrier damage caused by antibiotics. They found that hydrolyzed eATP enabled the expansion of the SIgA gene pool . Thus, intestinal health can be restored under abiotic conditions by combining SIgA with ATP hydrolysis.…”

Section: Intestinal Barrier Protection By Iapmentioning

confidence: 99%

Protective Properties of Intestinal Alkaline Phosphatase Supplementation on the Intestinal Barrier: Interactions and Effects

Gao,

Koko,

Hong

et al. 2023

J. Agric. Food Chem.

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The intestinal barrier is critical for maintaining intestinal homeostasis, and its dysfunction is associated with various diseases. Recent findings have revealed the multifunctional role of intestinal alkaline phosphatase (IAP) in diverse biological processes, including gut health maintenance and function. This review summarizes the protective effects of IAP on intestinal barrier integrity, encompassing the physical, chemical, microbial, and immune barriers. We discuss the results and insights from in vitro, animal model, and clinical studies as well as the available evidence regarding the impact of diet on IAP activity and expression. IAP can also be used as an indicator to assess intestinal-barrier-related diseases. Further research into the mechanisms of action and long-term health effects of IAP in maintaining overall intestinal health is essential for its future use as a dietary supplement or functional component in medical foods.

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Apyrase-mediated amplification of secretory IgA promotes intestinal homeostasis

Cited by 8 publications

References 73 publications

Integrated Mechanism of Immune Response Modulation by Arctium Lappa L. Fructans Based on Microbiome and Metabolomics Technologies

Integrated Mechanism of Immune Response Modulation by Arctium Lappa L. Fructans Based on Microbiome and Metabolomics Technologies

The P2X7 receptor in mucosal adaptive immunity

Protective Properties of Intestinal Alkaline Phosphatase Supplementation on the Intestinal Barrier: Interactions and Effects

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