AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes

Wang, Xiaoyu; Yang, Jing; Yao, Ying; Shi, Xin’e; Yang, Gongshe; Xiao, Li

doi:10.3390/genes11040453

Cited by 10 publications

(4 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was further suggested that melanin genes were involved in the body color variation of red tilapia during overwintering. Eleven known DEGs were shared by BP_PB, BP_WP and PB_WP comparison groups, of which st3gal1, plxna4, fabp11a and aqp3 plays a vital role in regulating cell proliferation, migration and invasion (Wu et al, 2018;Wang et al, 2020). It has been reported that silencing of st3gal1 gene suppresses melanoma invasion and signi cantly reduces the survive ability of aggressive melanoma cells of human in metastatic environment (Pietrobono et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of skin color variation during and after overwintering of Malaysian red tilapia

Jiang

Wang

Luo

et al. 2022

Fish Physiol Biochem

View full text Add to dashboard Cite

The commercial value of red tilapia is hampered by variations in skin color during overwintering. In this study, three types skin of red tilapia, including the skin remained pink color during and after overwintering (WP), the skin changed from pink color to black color during overwintering and remained black color after overwintering (PB), and the skin changed from pink color to black color during overwintering but recovered to pink color when the temperature rose after overwintering (BP), were used to analyze their molecular mechanisms of color variation. The transcriptome results revealed that the PB, WP, and BP libraries had 42, 43, and 43 million clean reads, respectively. The top 10 abundance mRNAs and speci c mRNAs (speci city measure SPM > 0.9) were screened. After comparing intergroup gene expression levels, there were 2528, 1924 and 1939 differentially expressed genes (DEGs) between BP and PB, BP and WP, and PB and WP, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of color-related mRNAs showed that a number of DEGs, including tyrp1, tyr, pmel, mitf, mc1r, asip, tat, hpdb and foxd3, might play a potential role in pigmentation. Additionally, the co-expression patterns of genes were detected within the pigment-related pathways by PPI network from PB_WP group. Furthermore, DEGs from the apoptosis and autophagy pathways, such as baxα, beclin1, and atg7, might be involved in the fading of red tilapia melanocytes. The ndings will aid in understanding the molecular mechanism underlying skin color variation in red tilapia during and after overwintering, as well as lay a foundation for future research aimed at improving red tilapia skin color characteristics.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of skin color variation during and after overwintering of Malaysian red tilapia

Jiang

Wang

Luo

et al. 2022

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Among genetic factors, genes can precisely regulate IMF accumulation in skeletal muscle. For instance, AQP3 promoted the proliferation and differentiation of porcine intramuscular preadipocytes [17]. The lipid synthesis was inhibited both in porcine subcutaneous and intramuscular preadipocytes after knockdown of CTRP6 [18].…”

Section: Discussionmentioning

confidence: 98%

miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking ANXA6 Expression

Lin

Tang

et al. 2022

Genes

View full text Add to dashboard Cite

Intramuscular fat (IMF) is one of the crucial factors determining meat quality. IMF deposition depends on the hyperplasia and hypertrophy of intramuscular preadipocytes, in which genes and noncoding RNAs play an important regulatory role. According to previous transcriptome analysis, ANXA6 and miR-24-3p were identified as involved in lipid metabolism in breast muscle. In this study, we further investigated their function in the proliferation and differentiation of chicken intramuscular preadipocytes. The results indicated that overexpression of ANXA6 inhibited proliferation and promoted differentiation of intramuscular preadipocytes, while knockdown of ANXA6 promoted cell proliferation and inhibited adipogenic differentiation. miR-24-3p was proved to directly bind to the 3′ untranslated region (3′UTR) of ANXA6 by dual-luciferase reporter assay. The regulatory effect of miR-24-3p on the proliferation and differentiation of intramuscular preadipocytes was opposite to that of ANXA6. Besides, the overexpression vector of ANXA6 eliminated the impact of miR-24-3p mimics on intramuscular preadipocytes. In brief, we revealed that miR-24-3p promoted proliferation but inhibited differentiation of intramuscular preadipocytes by blocking ANXA6 expression, thus dominating IMF deposition in broilers. These findings may provide a novel target for improving chicken meat quality.

show abstract

“…CD79α plays an important role in B cell development or the activation of downstream signals ( 56 ). AQP3 has a significant effect on the adipogenic differentiation and proliferation of intramuscular adipocytes ( 57 ). JPT2 is a microtubule-associated protein homolog that may have some effects on ASFV DNA and protein accumulation ( 50 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling Reveals Features of Immune Response and Metabolism of Acutely Infected, Dead and Asymptomatic Infection of African Swine Fever Virus in Pigs

et al. 2021

View full text Add to dashboard Cite

African swine fever virus (ASFV) infection can result in lethal disease in pigs. ASFV encodes 150-167 proteins, of which only approximately 50 encoded viral structure proteins are functionally known. ASFV also encodes some nonstructural proteins that are involved in the regulation of viral transcription, viral replication and evasion from host defense. However, the understanding of the molecular correlates of the severity of these infections is still limited. The purpose of this study was to compare host and viral gene expression differences and perform functional analysis in acutely infected, dead and cohabiting asymptomatic pigs infected with ASFV by using RNA-Seq technique; healthy pigs were used as controls. A total of 3,760 and 2,874 upregulated genes and 4,176 and 2,899 downregulated genes were found in healthy pigs vs. acutely infected, dead pigs or asymptomatic pigs, respectively. Additionally, 941 upregulated genes and 956 downregulated genes were identified in asymptomatic vs. acutely infected, dead pigs. Different alternative splicing (AS) events were also analyzed, as were gene chromosome locations, and protein-protein interaction (PPI) network prediction analysis was performed for significantly differentially expressed genes (DEGs). In addition, 30 DEGs were validated by RT-qPCR, and the results were consistent with the RNA-Seq results. We further analyzed the interaction between ASFV and its host at the molecular level and predicted the mechanisms responsible for asymptomatic pigs based on the selected DEGs. Interestingly, we found that some viral genes in cohabiting asymptomatic pigs might integrate into host genes (DP96R, I73R and L83L) or remain in the tissues of cohabiting asymptomatic pigs. In conclusion, the data obtained in the present study provide new evidence for further elucidating ASFV-host interactions and the ASFV infection mechanism and will facilitate the implementation of integrated strategies for controlling ASF spread.

show abstract

AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes

Cited by 10 publications

References 41 publications

Transcriptome analysis of skin color variation during and after overwintering of Malaysian red tilapia

Transcriptome analysis of skin color variation during and after overwintering of Malaysian red tilapia

miR-24-3p Dominates the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes by Blocking ANXA6 Expression

Transcriptome Profiling Reveals Features of Immune Response and Metabolism of Acutely Infected, Dead and Asymptomatic Infection of African Swine Fever Virus in Pigs

Contact Info

Product

Resources

About