AQP7 Up-Regulation in the Skeletal Muscles of Mice with Diet Induced Obesity

Wakayama, Yoshihiro

doi:10.4172/2157-7013.1000205

Cited by 3 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, it was hypothesized that AQP7 and AQP9 mRNAs expression would be altered in the obese db/db mouse skeletal and cardiac muscles, since the neutral lipid, in which the triglyceride was a main substance, was increasing in these cells. As was observed in the skeletal muscles of obese ob/ob mice [ 33 ] and in diet induced obesity mice [ 32 ], upregulation of AQP7, but not AQP9 mRNAs in skeletal and cardiac muscles of db/db mice was revealed in this study. Increased AQP7 abundance was also described in skeletal muscle from obese men with type 2 diabetes [ 19 ].…”

supporting

confidence: 80%

“…An increase in lipid content has been reported in the skeletal muscle cells of obese animals [ 14 , 23 , 28 , 32 , 33 ]. The increased lipid content, such as triglycerides, in the skeletal and / or cardiac muscle cells is synthesized with glycerol and fatty acids in the muscle cells.…”

mentioning

confidence: 99%

“…Type 1 fibers have an enhanced capacity for mitochondrial respiration and fatty acid oxidation and contain slow isoforms of contractile proteins (slow myosin); whereas type 2 fibers preferentially oxidise glucose and contain fast twitch contractile proteins (fast myosin) [ 30 ]. Intramyofiber lipid droplets are increased in the skeletal muscle cells of obese animal [ 5 , 32 , 33 , 39 ]. The fatty acids derived from the intramyocellular lipid droplets are metabolized in the mitochondria by β-oxidation.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese <i>db/db</i> mice

Wakayama

Hirako

Ohtaki

et al. 2021

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

The aim of this study is to examine 1) muscle fiber type composition, 2) myofiber diameter, and 3) aquaglyceroporin (AQP) and AQP 9 mRNA expressions by quantitative PCR in muscles of obese db/db mice. The myofiber type composition of skeletal muscle was not statistically significantly different between db/db mice and control mice; while the average myofiber diameter ratio showed a decrease in db/db mice. The expression of AQP7 but not AQP9 mRNA in the skeletal and cardiac muscles was significantly upregulated in db/db mice. Thus this study revealed quantitatively that type 2 myofiber atrophy was shown in the skeletal muscles of db/db mice. AQP7 mRNA expression was upregulated in the skeletal and cardiac muscles of db/db mice.

show abstract

supporting

confidence: 80%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese <i>db/db</i> mice

Wakayama

Hirako

Ohtaki

et al. 2021

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…AQPs are intrinsic membrane proteins that form channels in epithelial and endothelial cells [22]. AQP7 apparently regulates the efflux of lipolysis-derived glycerol from adipocytes, which are essential in the maintenance of normal adiposity [22]. A previous study had shown that AQP7 is up-regulated by fasting, low insulin and PPARα and altered expression may be related to lipid metabolism disorders [23].…”

Section: Discussionmentioning

confidence: 99%

Hepatic transcriptome profile of sheep (Ovis aries) in response to overgrazing: novel genes and pathways revealed

et al. 2019

View full text Add to dashboard Cite

Background Overgrazing is a major factor that causes steppe degradation in Inner Mongolian, resulting in extensive ecosystem damage. Scarcity of grass means sheep are smaller and therefore mutton and cashmere production is greatly reduced, which has resulted in massive annual economic losses. Liver is the primary metabolic organ in mammals. It is also the key source of energy supply and detoxification of metabolites in animals, has a close relationship with animal growth. However, investigations on the responses of sheep induced by consequence of overgrazing, particularly those relating to liver-related molecular mechanisms and related metabolic pathways, remain elusive. Results The body weight daily gain of sheep, immune organ indices (liver and spleen), and serum parameters related to immune response, protein synthesis and energy supply (IgG, albumin, glucose and non-esterified fatty acid) were significantly lower in the overgrazing group. Other serum parameters including alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, blood urea nitrogen and interleukin-6 were significantly higher in the overgrazing group. For the RNA-Seq results, we identified approximately 50 differentially expressed genes, of which half of were up-regulated and the other half were down-regulated (overgrazing group versus light grazing group). Bioinformatics analysis identified two enriched KEGG pathways including peroxisome proliferator-activated receptor (PPAR) signaling pathway (related to lipolysis) and ECM-receptor interaction (related to liver injury and apoptosis). Additionally, several of the down-regulated genes were related to detoxification and immune response. Conclusions Overall, based on the high-throughput RNA sequencing profile integrated with the results of serum biochemical analyses, consequences of lower forage availability and quality under overgrazing condition induced altered expression levels of genes participating in energy metabolism (particularly lipid metabolism) and detoxification and immune responses, causing lipolysis and impaired health status, which might be key reasons for the reduced growth performance of sheep. This investigation provides a novel foundation for the development of sheep hepatic gene interactive networks that are a response to the degraded forage availability under overgrazing condition. Electronic supplementary material The online version of this article (10.1186/s12863-019-0760-x) contains supplementary material, which is available to authorized users.

show abstract

Cardioprotective effect of St. Thomas’ Hospital No. 2 solution against age-related changes in aquaporin-7-deficient mice

Fujii,

Bessho,

Akimoto

et al. 2023

Gen Thorac Cardiovasc Surg

View full text Add to dashboard Cite

Objective This study aimed to investigate whether St. Thomas’ Hospital No. 2 solution (STH2) is equally effective in both young and aged aquaporin-7-knockout (AQP7-KO) mice and the mechanisms by which the intra-myocardial adenosine triphosphate (ATP) content is altered during ischemia without aquaporin-7. Methods In study 1, isolated hearts of male wild-type (WT) and AQP7-KO mice (< 12 weeks old) were Langendorff perfused with 5-min STH2 prior to a 20-min global ischemia (GI) or 25-min GI without STH2. Similarly, in Study 2, hearts from WT and AQP7-KO mice (≥ 24 weeks old) were subjected to 2-min STH2 infusion prior to GI. In study 3, intra-myocardial ATP content was compared before (sham) and after (control or STH2) ischemia in mature WT and AQP7-KO mice. Results In study 1, troponin T levels (ng/g wet weight) of WT and AQP7-KO hearts were significantly lower in the STH2 groups (75.6 ± 45.9 and 80.2 ± 52.2, respectively) than in the GI groups (934.0 ± 341.1 and 1089.3 ± 182.5, respectively). In Study 2, troponin T levels in aged WT and AQP7-KO mice were 566.5 ± 550.0 and 547.8 ± 594.3, respectively (p = 0.9561). In Study 3, ATP levels (μmol/g protein) in the sham, control, and STH2 AQP7-KO mice groups were 4.45, 2.57, and 3.37, respectively(p = 0.0005). Conclusions The present study revealed the cardio-protective efficacy of STH2 in an experimental model of isolated AQP7-KO young and aged murine hearts. Further, STH2 preserved intra-myocardial ATP during ischemia with Krebs–Henseleit buffer perfusion in the Langendorff setting.

show abstract

AQP7 Up-Regulation in the Skeletal Muscles of Mice with Diet Induced Obesity

Cited by 3 publications

References 32 publications

Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese <i>db/db</i> mice

Histopathological and aquaporin7 mRNA expression analyzes in the skeletal and cardiac muscles of obese <i>db/db</i> mice

Hepatic transcriptome profile of sheep (Ovis aries) in response to overgrazing: novel genes and pathways revealed

Cardioprotective effect of St. Thomas’ Hospital No. 2 solution against age-related changes in aquaporin-7-deficient mice

Contact Info

Product

Resources

About