Aquaporin 1 (AQP1) Expression in Healthy Dog Tears

Lamagna, Barbara; Ciaramella, Paolo; Lamagna, Francesco; Loria, Antonio Di; Brunetti, Arturo; Pelagalli, Alessandra

doi:10.3390/ani10050820

Cited by 8 publications

(11 citation statements)

References 48 publications

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“…In veterinary medicine, recent interesting investigations regarding canine tears have been reported, such as the presence of AQPs (AQP0, AQP1, AQP3–5 and AQP9) in different canine ocular tissues [ 6 ]. The expression of aquaporin-1 (AQP1) by Western blot analysis was found in canine eye tears, suggesting its specific role in tear secretion [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Dexmedetomidine and Tear Production: Evaluation in Dogs as Spontaneous Model for Ocular Surface Disorders

Pietro

Giannetto

Falcone

et al. 2021

Veterinary Sciences

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Tear film provides lubrication and protection to the ocular surface. The sedation reduces tear production, often leading to perioperative exposure keratopathy. The aim of the present study was to report the effects of intramuscular dexmedetomidine on canine tear production, measured by STT-1, for an experimental period of 8 h after sedation. Ten dogs who underwent sedation for routine radiologic assessment were recruited for the study. In all animals, tear production in right and left eyes was measured 15 min before sedation (T0: basal values) and 20 min (T20), 1 h (T1), 2 h (T2), 4 h (T4) and 8 h (T8) after drug administration. Analysis of variance and post hoc Bonferroni test (p < 0.05) were performed. A significant effect of time on canine tear production was found. The tear production returned to basal values at T8. So, it is recommended to treat the canine eyes with tear substitutes during and up to 12 h after sedation.

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Section: Introductionmentioning

confidence: 99%

Dexmedetomidine and Tear Production: Evaluation in Dogs as Spontaneous Model for Ocular Surface Disorders

Pietro

Giannetto

Falcone

et al. 2021

Veterinary Sciences

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“…In fact, Schirmer strips have been used for comprehensive proteomic studies in dogs 9,39 and identification of individual proteins such as aquaporins in tears. 40…”

Section: Discussionmentioning

confidence: 99%

Impact of diurnal variation, sex, tear collection method, and disease state on tear protein levels in dogs

Page

Allbaugh

Mochel

et al. 2020

Veterinary Ophthalmology

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Objective To investigate the effects of various biological factors on total protein concentration (TPC) and serum albumin levels in canine tears. Animals studied 10 healthy beagles (5 female, 5 male) were used. Procedures Experiments were conducted on separate days, collecting tears with either capillary tubes or Schirmer strips, as follows: (i) Tear collection at 3 hours intervals (from 6 am to 12 am); and (ii) Tear collection before and 20 minutes following topical histamine application (1, 10, 375 mg/mL) to induce mild, moderate, and severe conjunctivitis, respectively. TPC and serum albumin were measured with infrared spectroscopy and ELISA, respectively. Results Tear film TPC and serum albumin ranged from 9.7‐26.1 mg/mL and 6.4‐1662.6 µg/mL, respectively. Protein levels did not differ significantly among time points (P ≥ .080). Median coefficient of variation (CV%) was lower with Schirmer strips compared to capillary tubes for both TPC (12% vs 15%, P = .020) and serum albumin (57% vs 78%, P = .232). TPC (P < .001), but not serum albumin was greater in male vs. female dogs. Serum albumin, but not TPC (P ≥ .099), increased significantly with each grade of conjunctivitis severity (P < .001), with no differences between collection devices (P ≥ .322); median increase was 106%, 1389%, and 2871% in eyes with mild, moderate, and severe conjunctivitis, respectively. Conclusions There is no apparent diurnal variation in canine tear protein levels. Blood‐tear barrier breakdown with conjunctivitis allows serum albumin to leak into the tear film at high concentrations. Schirmer strips compare well with capillary tubes for bioanalytical purposes in healthy and diseased eyes, and this collection method may offer improved reproducibility for protein quantification.

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“…The collected data indicate the role of AQPs in water management in a dog's eye. AQP1 has also been shown to play an important role in producing tears in healthy dogs (37). It is likewise suggested that deficiency of AQP5 in the lacrimal glands and nictitating membrane glands in dogs may be correlated with keratoconjunctivitis sicca (71).…”

Section: Aquaporins In Caninesmentioning

confidence: 99%

Potential of aquaporins and connexins in dogs and their relation to the reproductive tract

Kulus¹,

Wieczorkiewicz²,

Kulus³

et al. 2021

Medycyna Weterynaryjna

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The complexity of processes in the female reproductive system of mammals is extremely sophisticated. The overall relationship between the processes during the oestrus cycle in animals is quite well understood, but the molecular background of these processes still requires an in-depth analysis. Bitches are distinguished by a specific course of sexual cycle during which the oocyte matures after ovulation in the oviduct. Other species of mammals are characterized by maturation of the oocyte within the ovary. Acquisition of developmental competence by cumulus – oocyte complexes seems to be a process with a complex molecular background, and the key to understanding it may be the analysis of intercellular channels. Aquaporins and connexins are structural proteins that are built into the cell membrane. Their location is widespread in many body tissues. Recent years have shown that they exhibit significant expression in different parts of the mammalian reproductive system, although the number of studies on dogs is still negligible. This review paper presents the current state of knowledge of water channels and gap junction connections in different animal species, with particular focus on dogs, and also explores the role of aquaporins and connexins in the acquisition of reproductive competences.

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Aquaporin 1 (AQP1) Expression in Healthy Dog Tears

Cited by 8 publications

References 48 publications

Dexmedetomidine and Tear Production: Evaluation in Dogs as Spontaneous Model for Ocular Surface Disorders

Dexmedetomidine and Tear Production: Evaluation in Dogs as Spontaneous Model for Ocular Surface Disorders

Impact of diurnal variation, sex, tear collection method, and disease state on tear protein levels in dogs

Potential of aquaporins and connexins in dogs and their relation to the reproductive tract

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