2006
DOI: 10.1159/000099625
|View full text |Cite
|
Sign up to set email alerts
|

Aquaporin 1: Examination of Its Expression and Localization in Normal Human Skeletal Muscle Tissue

Abstract: To examine aquaporin 1 (AQP1) expression in skeletal muscle tissue precisely, we performed reverse transcription-polymerase chain reaction (RT-PCR) at RNA level and immunoblot analysis, immunohistochemistry and immunoelectron microscopy at protein level. The RT-PCR study of total RNA from normal human skeletal muscle showed a strong single band of AQP1. At the protein level we used two commercially available antibodies, both of which recognize the cytoplasmic domain of the AQP1 molecule. One antibody gave posi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
5
0

Year Published

2008
2008
2023
2023

Publication Types

Select...
10

Relationship

1
9

Authors

Journals

citations
Cited by 12 publications
(6 citation statements)
references
References 27 publications
1
5
0
Order By: Relevance
“…Additional studies that have focused specifically on aquaporin-1 have shown the absence of aquaporin-1 on adult mouse muscle fibers (98), and similar results have been observed in rat (99), although its presence has been reported for human adult skeletal muscle (100, 101). The mRNA levels for aquaporin-1 were previously found to decrease significantly with myoblast differentiation (55, 56).…”
Section: Discussionsupporting
confidence: 55%
“…Additional studies that have focused specifically on aquaporin-1 have shown the absence of aquaporin-1 on adult mouse muscle fibers (98), and similar results have been observed in rat (99), although its presence has been reported for human adult skeletal muscle (100, 101). The mRNA levels for aquaporin-1 were previously found to decrease significantly with myoblast differentiation (55, 56).…”
Section: Discussionsupporting
confidence: 55%
“…Water permeation across the sarcolemma of skeletal muscle is mediated by aquaporins (AQP). AQP exist in several isoforms, among which AQP1, -3, and -7 are expressed in adult rat skeletal muscles (382,840,841) with no difference among fiber types. In contrast, expression of AQP4 is restricted to fast-twitch fibers of mammalian skeletal muscles, and such selective expression might account for the faster osmotic response of rat EDL compared with soleus (236).…”
Section: Trans-sarcolemmal Substrate Transportmentioning
confidence: 99%
“…The immunoblotting was performed by a method previously described, 20 with minor modifications. A total of 20 mg of protein was separated by SDS-PAGE.…”
Section: Immunoblottingmentioning
confidence: 99%